LOCUS       sequence-306523-        1840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018271
FEATURES             Location/Qualifiers
     source          1..1840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1788..1808
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1809..1832
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgacgcggaa gcagtggttc ctttccggct tcgtcgcctt cacctgcctg gcctccactg
      121 cggccggcgc cgggccgatc gagaactata gccccgtgac cgcgcagcgt ctcgaaaatc
      181 ccgagccctc gaactggatg ctgtaccgcc gcacctatga tggccagggc tattcgccgc
      241 tcgatcagat caacacctcg aacgtcaagg gcctcacccc cgtgtggacc ttcgccacgg
      301 gcgtcgtcga gggccatgag gccccgccaa tcgtcaacaa cggggtgatg ttcgttgcga
      361 cgcccatggg acaggtcatc gccctgaacg ccaagaccgg cgacgagtac tggcgctaca
      421 agcgacagct ccccgatgac ctcttccaac tgcacccgac ctcgcggggc gtgggcctct
      481 gggaggacaa gctgtacctg gccaccaccg atgatcacgt ggttgccctc gatgccaaga
      541 ccggcaaggt ggtttgggac acgaaggtcc aggactacaa gaagggccag tacatgaccc
      601 tcatgccgct gatcgtcgac ggtaaggtga tcgtcggtgg ctcgggcggc gagttcggcg
      661 tgcgtggata cgtcgcggcg tacgatgcca aggacggcaa ggagctctgg cgcacctaca
      721 ccatccccgg cgagggtgag ccgggccacg atacctggca gggcgacgac tggaagaacg
      781 gcggcggctc ggcctggatg accggcaact atgacaagga caccaagacc atctactggg
      841 gcgtcggcaa cgcggcgccg tggcccggcg aaacccaccc gggcgacaat ctctatacca
      901 gctccgtcct ggcgcttgac cccaacaacg ggaagatcaa aacctatcat cagtaccatc
      961 aaaacgacag ctgggactgg gatgaggtcg aggcccccat gctcatcgac cttcagcgtg
     1021 atggccgcag catcaagtcc ctggtccacc ctggccgcga cgccatcttc tgggtcctgg
     1081 agcgcacccc gacgaagatc aattacgtcg caggctggcc cttcgtgtcc acggacgtct
     1141 ggaagggcat agacgcggaa acgggcaagc cgatcgtcga cccggcccat aagcccgtca
     1201 tcggcaagcg cgtcgagttt tgcccctctt tgtggggcgg gaaggactgg ccgagcgccg
     1261 cctacagcca gaagaccggg ctcgtctatg tcccggcgaa cgagaacttc tgcggcggct
     1321 tcaccggcga aaaggtggcc ctgaagccgg gcgaactgtg gctggggacc aagccggaag
     1381 acatcggact gaagaccaag ccgggagccg accacttcgg cgaactccag gcgtgggacc
     1441 ctgtcaccgg caagaaagtg tggcagcata acttcccgaa atcgcagctt ttcggcagcg
     1501 tgaccgccac agcgggcgat ctgatcttcg ccgggggcac gaacgaccga aacttccggg
     1561 cctttaacgc caagacaggc gaactgctct gggagcagaa gaccaacagc ggcatcatgg
     1621 gcatgccggt gtcctatgag atcgatggta ctcagtacat cgccatccag tccggctggg
     1681 gcgtcgatgc ccaacgcatc caggatgccc tggtcactaa caacatcggc atagaggcca
     1741 acgtccctca gggcggtgtg atctgggtct tcgccctgaa gaaggccgaa aacctctatt
     1801 ttcagggaca tcatcaccat caccatcacc actgacagct
//
