LOCUS       sequence-306556-        1936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018337
FEATURES             Location/Qualifiers
     source          1..1936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1884..1904
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1905..1928
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgctccttgc cctggcggtg ggcacggcga ccgtcatccc gtcggcctac tcgaacgacg
      121 acgtcctcaa gctcaccgag aacccgaaga actgggcggc ccccggcaag gattacgcca
      181 acacgcgcca ctcgtccctc aagcagatca atacccagaa cgtcaagagt ctccacatgg
      241 cctggtcatt ctcgacgggg acgcttcgcg gccacgaggg ccagcccctg gtcgtcggcg
      301 atcggatgta cgtggtgagc agctacccga acatcgtctg ggcgctcgac ctgtccaagg
      361 gtggctcgta tgaggtgctc tggaagtatg tcccccgcca agatgataag gccgtcagca
      421 ccgcatgctg cgacacggtg aaccgcggcg cgtcctacgc cgatggcaag gtcgtcttca
      481 acacgctgga cggctacgtg gtgtgcctgg atgcgaacac cggcaaggag ctctggaaga
      541 cgaagttcgc cgacctcaac aagggcgaga ccagcacccc cgcgcccatc atcgtcaaag
      601 acaaggtctt caccggctac ggcggtgatg agttcggggc gcggggccgc ttcgccgcct
      661 tcgacctaaa cagcggcaaa atggtctggc aggcgtatag caacggcccg gactcggatg
      721 tccttctcgg gcccgacttc aactcgaaac atccggagta cggccaggcc ggccaggatc
      781 tcggcgtgaa gacctacccg gacgaggagt ggaaacgggg cggtggctgc gcgtggggct
      841 ggtactcgta cgacccgaag ctcgacctgg tgtactacaa cacgggcaac ccggggctgt
      901 ggagcccgtc gtaccgcaca gaggcgaaga cccacgagga agccaacgag ccatggaagt
      961 gggacaataa gtggtccatg accgtcttcg cccggaagcc agataccggc gaagcggtct
     1021 ggggctacca gctcaccccc ttcgaccagt gggactacga tgccatcaac gaggacgtcc
     1081 tggtcgacat caccgtcgac ggcgcgaaga agccttgcct cgtccacttc gaccggaacg
     1141 gcttcgccta cgtgctctcg cgcacggatg ggaccctcat ccgggccaac aaattcgtca
     1201 ccgtcaactg ggcggaaaaa atcgatatga agaccggccg gccggtcaag gtgaaagagc
     1261 actcgccgtt cgaggttgga aaggcggtcc aggcgtaccc tagcgcccag ggcggcaagg
     1321 atcaacagcc ggtcgcggtc gatccgaagg aggccaacgt gttctacgcc cccaccaaca
     1381 actggggcat gaccctggag ccgatggagc gcgcccacac gaaccaaggt tcggtctacg
     1441 tgttcgcgaa tgtgctgatg aagcccgaga agcccggcgt aatgggccgg ttcaaggcct
     1501 tcgatgtcat cacggggaag gcccgttggg acatcccgga gcggttcccg acctggtccg
     1561 gggccctggt cacggatggc ggactggcgt tctacggcac gctggacggc tggttccgcg
     1621 ccgtcgatcg caagaccggc aaggtgctct ggcagcagaa gctcggctcg ggcatcatcg
     1681 gcaaccccat ctcgttcgag gtgggcggca agcagtatat ctcgatcctc acgggcatcg
     1741 gcggctggat cggccttccc gtcacggccg gccttgaccc agccgacccg tacggcgcgc
     1801 tgggcgtggc gggcatggcc ggcgagaacg gcttctacaa catcccgatg ggaggcacgc
     1861 tctacacatt ctgcgtccaa gccgaaaacc tctattttca gggacatcat caccatcacc
     1921 atcaccactg acagct
//
