LOCUS       sequence-306561-        1954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018347
FEATURES             Location/Qualifiers
     source          1..1954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1902..1922
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1923..1946
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgagcgtcaa gcgccgcatg tcgctgatcg gtgggctgct cgtgctggcc ggctcgctcg
      121 cgatctcggc gccgatcgtc tcggcgaacg atgacgtcct gaaactgcag aaggatgatg
      181 gccagtgggt ggtccagtcg aagaactact cgggaacacg ctattcgact ctgaaccaga
      241 tcaccccgga gaacgcaaag aacctgaagg tggcctggtc gttctccctt gggaccctca
      301 acggccagca ggggggccct ctggtcgtcg gcgatacaat gtatgctcac tcgagctacg
      361 cgggcggccc gacggggcat agccataaca tcttcgccct cgacctctcc aaggagggtg
      421 caccgatcaa gtggcgctac aacgcgaagt acgaccagaa ggcccccccg gtcgcctgct
      481 gcgatctcgt gcataagggc ctccagtacg ccaacggcaa gatcctctac cagaccctgg
      541 atggcatggt catcgccctc gatgccaaga cgggcaagga gctgtggaag actcgcaacg
      601 ccgacccctc caagggcgag acgtccacgg ccgccggctt ggtgatcaag gacaagtaca
      661 tcgcgggtgt cgccggcggc gaattcggcg tgcgcggctg ggtcgccgcc tacgatatta
      721 acacgggcaa gcaggtgtgg aaggcgcggt cgatgggccc ggacgaggag atcaagctcg
      781 cgccggattt caacgcggcg aacccccact acggccgctt cggggagggt accaaatcat
      841 ggccggggga gatgtggaag cagggcggcg gcactacctg gggatattac gcctatgacc
      901 cggagctcaa cctgctctat tacgggtccg ggaatccggg cacctggaac ccggcgccgc
      961 gcaagggcgg ggacaacaag tggtccatga cgatctgggc ccgcaacccg gataccggcg
     1021 tcgccaagtg ggcgtaccag atgaccccct gggacaactg ggactacgat ggcatcgatg
     1081 aatcggttct caccgagcag accttcggag gcaagaagcg gaaggtcctg acccatttcg
     1141 accgcaacgg cttcgtctac acgctggacc gcgccgatgg taccctcctt gccgcggatg
     1201 ccttcgtcta cgtcaactgg gcgaagggga tcgacctcaa gaccggccgc ccgatcctca
     1261 acccagagaa gctgacaaag cagggcgagg acaccaagaa tatctgcccg tgcgcgatgg
     1321 ggggaaagaa ctacctgccg gtcgcctaca gcccgctcac caacctgttc tatgccagcg
     1381 tgaataacat gtgcatgagc tatgaggggg tgcaggtcca gtataccgcc ggcgcgccgt
     1441 acgtcggcgc caccgtcctg atgtacgaag gccatgaggg caaggacgtc ggctactggg
     1501 gggacttcat cgcctgggac gtcgtaaagg ggaagagggc gtgggccatc aaggagagcc
     1561 agcccccgtg gtcgggcccg gtcgtgacct cgactaatgt ggtgttctac ggcacgatgg
     1621 atggctggtt caaggcggtc gatgcccggg atggcaagct cctctggaag cacaaggtcg
     1681 gcagtggcat catcggcaac ccgatcacct acaagggccc tgacggcaag cagtacgtcg
     1741 cggtgtattc cggcgtgggc ggctggttcg gcgccacggt gtccttggac ctgccgccgg
     1801 acgacccgac cgctgcgctc ggcggcgtga atgccgcgta caagtccggg ctccctatgg
     1861 ccaccacgaa gggcgggacc ctctacgtgt tcggtctggc cgaaaacctc tattttcagg
     1921 gacatcatca ccatcaccat caccactgac agct
//
