LOCUS       sequence-306564-        1963 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018353
FEATURES             Location/Qualifiers
     source          1..1963
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1911..1931
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1932..1955
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatataccg
       61 tgcatcagta caaggacttc ttcctttata gcatccttct actgatcggc ttcctcaccg
      121 cggcgtgctc gcaggactcc gcgccgccca accccgacag ctcccccccg tccgtggccc
      181 agggaaacta cccgcacaac aatagcagcc cgccgacctg gctcgaggcg gacgaggacg
      241 gccagtggac catgccggcc aaaaactacg cgtccacgcg ctacagcggc ctgtcccaaa
      301 tcaatcggga caatgtcaag gatctcaagc tcgcctggac gttcagcacc ggcctcacgc
      361 cgggccacga ggcggccccg gtgttcgtcg acgataccct gtacgtcgtc acgccctacc
      421 ccaacaccgt gtacgcactc gcactggacg gcagcaagaa gtgggagtac aagccggaca
      481 cccatcagga gagccagggc atggcctgct gcgacgttgt caatcgcggc ctcgtccacg
      541 tgaacggcaa gctattcttt aacaccttgg acaaccacgc gatcgccctc gaagccgaca
      601 ccggcaagga gctctggaag accaaggtgg gcgacctgca gcggggcgaa accatgacca
      661 tggccccgct cgtcgtcaag gggaaaatgc tcgtcggaaa ctcgggcggc gagatgggcg
      721 tgcgcggctg gctgaaggcg ctggacacgg agaccggcga gctgctgtgg accgcgtact
      781 cgaccggccc ggataaggac gtcctgatcg gcgaggagtt ccgcccgttc tacgcacagg
      841 accagggcaa ggaccttggg gccagctcct ggccccccga gatgtggaag atcgggggcg
      901 gcaccgtgtg gggctggctg tcgtacgatc cggagctgga tctcgtgtac tacggcaccg
      961 ggaacccggg cccgtggaac ccggacttcc gacccggcga caacaagtgg accctgaccg
     1021 tcttcgctcg ccgccccgac gacggctccg ccatctgggc ctatcagatc gagcctcacg
     1081 acctctacga ttacgatggg gtcaacgagt cgctcctgat cgacatgccc ttcaacgggg
     1141 agcagcgcaa ggtcctggtg cgtccggagc gcaatggcca cgtgtacgtc atggaccgca
     1201 ccagcggcga ggtcctgagc gcgaatacct atgtcccggt gaacgtcacc gacggggtgg
     1261 atctgaagac cggccagatg cgcaaggtga aggataagca gccggggacc aaccggacag
     1321 tgcataacat ctgcccggcc gcccctggcg gcaaggactg gcaaccgtct gcgtactccc
     1381 cccgcacggg gctcatttac atgcctcaca acaacttgtg catggacatg cacggcacgc
     1441 aggccaacta tatcgcgggt acgccctacg tggggtccga cgtcaagatg ttcgccggcc
     1501 cgggcggcca ccggggcgcc tacaccgcct gggatccggt cgcgggctcg aaggtctggt
     1561 ccatcaagga ggagttcccg gtgtggagcg gtacggtggc caccgcgggc gacatcgtct
     1621 tctatggcac catggatcgt tggttcaagg cgctcgatgc gaagactggc gagctactct
     1681 ggaagtaccg ggtcggctcg ggcatcgtcg gtcagccggt caccttccaa gataaggacg
     1741 ggaagcagta tgtcgccatc ttctctggcg tcggcggctg gtcgggcgcc atcgtgtccg
     1801 gcggcctgga cgcccgtgac ggcaccgccg cactgggctt cgtcaacgcg atgaaggacc
     1861 tcccgcaaca taccgacaag ggcggccagc tctatgtctt cagcttggcc gaaaacctct
     1921 attttcaggg acatcatcac catcaccatc accactgaca gct
//
