LOCUS       sequence-306567-        1972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Rare earth element alcohol dehydrogenases.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Huang J, Yu Z, Groom J, Cheng JF, Tarver A, Yoshikuni Y, 
            Chistoserdova L
  TITLE     Rare earth element alcohol dehydrogenases widely occur among 
            globally distributed, numerically abundant and environmentally 
            important microbes.
  JOURNAL   ISME J. 2019 Aug;13(8):2005-2017. doi: 10.1038/s41396-019-0414-z. 
            Epub 2019 Apr 5.
  PUBMED    30952993
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41396-019-0414-z"; journalName: "ISME J"; date: "2019-08";
            volume: "13"; issue: "8"; pages: "2005-2017"
COMMENT     Sequence Label: JGI_018359
FEATURES             Location/Qualifiers
     source          1..1972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..53
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1920..1940
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1941..1964
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 gacttgtcgg aggaatcctc tagaaataat tttgtttaac tttaagaagg agatatacca
       61 tgaagtttca tcgggtcgcg atggtgtgca cggcggttct aatgggggcc gtcggcggaa
      121 atgccatggc caaccccgag accatccagc gccaaaagga cccgaaccag tggcccgcgc
      181 cgggccgcga cctcgccatg acccgtcact ccgccctgaa agacatctca acggagaacg
      241 tgggaaagat gcagatgatc tggtcgcaga gctccggcac cctccgtggc cacgagggcc
      301 agccggtcgt cgtgaccgag ggcggcaagg cgatgatgta tctcatctcc ggctggccta
      361 acattgttca ggcgctcgac ctcagcgacc cggacaaccc caaggccatc tggaagtacg
      421 tcaaacagac ggatcgggat gagtccgccg ttccacgcgc ctgctgcgac accgtcaatc
      481 gcggtctgag ctacgctgac gggaaggtcg tcttcggcac gcttgacggc ttcgtcatcg
      541 cgctggacgc gaagacgggg aaggaggtgt gggtggtcaa gcacgccttc cctgagaacg
      601 gtgagactat cacgccggcc ccgctcatcg ccgagaacaa ggtgctcatc ggcttcggcg
      661 gcgatgagtt cgccgcgcgc ggccgcttca ccgcctacaa cctcgccgac ggcaagaagc
      721 tgtgggagtg ccatgccacc gggccggata aggatgtgtg cgtgaccaag aacacgaaca
      781 aggcgaaccc gcattacggc caaggcggca aggacctcgg ggtgacgggc tacccgggtg
      841 acgaatggaa gcgcggcggc ggcgcgagct ggggcttcta tagctacgac ccggagctca
      901 agctcgtcta tcacgcgacg ggcaacccgg gactgtggag cccctcctat cggtgcaagc
      961 ataagacgca cgaggagtgc aacgacggct cgcaggataa ccactggtcg atgaccatct
     1021 tcgcgcgcaa ggtcgacacc ggcgaggccg tctgggcgta ccagatgacg cccttcgacc
     1081 agtgggatta cgacggcatc aacgaaaaca tgctcgccga ctggaccatc gacggtaaga
     1141 agcgcaaggt cctggtgcac ttcgaccgca acggtttcgc ttacgtcctc gaccgtgtcg
     1201 acggcacgct cctgaaagcg cacaagtacg tcaccgcaaa ctgggccgag aaagtggaca
     1261 tgaagaccgg caagccggtg aaggtcaagg agcactcgcc gttctcggtg gagcagaata
     1321 cccaagtgtg cccgtccgcc atgggcggca aggaccagca gccctgcgcg ctggacccga
     1381 atgaagaagg agtcgcgtac tgcccgacca acaactggtg catggaactg gagccgcagg
     1441 agcgggccca cacgcagcaa ggcaccgtct atgtgttcgc taacgtgtac atgtacccgg
     1501 agaagccggg cgtcaccggc aagttcaaga agttcaacgt gctgaccggc gagaccgtgt
     1561 gggagatccc cgacccgtat cccaattggg gcggcgccct tatcacagac ggtggcctgg
     1621 cgttctatgg ctcgctgggc ggcgacttcc gcgcggtgga ccgcaagacc ggtaaggtgc
     1681 tctggagccg caagctcggc tccggcatca tcgggaaccc catcacgtac aaggtcaacg
     1741 ggaagcagta cgtcagcgtc tggtccggta tcggcggctg gatcggcctc ccagttaccg
     1801 ccggcctgga cctcgaggac aagttcggcg ccatcggggc gaccgcgatg accaaggccg
     1861 ccaacctcga taagattcct caggggggca ccctgtacac cttccgcatc ggcgacgccg
     1921 aaaacctcta ttttcaggga catcatcacc atcaccatca ccactgacag ct
//
