LOCUS       sequence-307083-        2319 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom terminator for P. tricornutum; uLoop specific syntax; EF 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_EF_tSVP
FEATURES             Location/Qualifiers
     source          1..2319
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(827..1415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(907..926)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1506..2297)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcttgatcag atattgatcc cctgcgccat cagatccttg gcggcaagaa agccatccag
       61 tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa ttccggttcg
      121 cttgctaagc ttgcatgcct gcaagtcgac tctagaggag cggtgatcac aggcagcaac
      181 gctctgtcat cgttacaatc aacatgctac cctccgcgag atcatccgtg tttcaaaccc
      241 ggcagcttag ttgccgttct tccgaatagc atcggtaaca tgagcaaagt ctgccgcctt
      301 acaacggctc tcccgctgac gccgtcccgg actgatgggc tgcctgtatc gagtggtgat
      361 tttgtgccga gctcattact cgcatccatt ctcaggctgt ctcgtctcgt ctcaggtctc
      421 cgcttatgct ggatttcttg cgacgtaaaa atattctata gagcagcctt gtggttgtca
      481 tgaaactgac ttattactcg tagttaatat ggatgaatta atggtgaggg ttgtattccg
      541 atcttcgagg aaaattcttc gctttacgtt gtctctggaa gccctactgt ggcataccgc
      601 acatgctcgt gccaataaac gaaatccccc tcctgcttga tgaatgctgc cgatactata
      661 atataattgc cttgaaatgg tgcacgctcg agaccaccag gatacataga ttaccacaac
      721 tccgagccct tccacccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      781 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      841 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      901 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      961 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1021 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1081 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1141 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1201 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     1261 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     1321 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     1381 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     1441 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
     1501 gattattatt tgccgactac cttggtgatc tcgcctttca cgtagtggac aaattcttcc
     1561 aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc ctgtctagct
     1621 tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc ggcagcgaca
     1681 tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa cgtaagcact
     1741 acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa ggtttcattt
     1801 agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc cgctggacct
     1861 accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc aatgtcgatc
     1921 gtggctggct cgaagatacc agcaagaatg tcattgcgct gccattctcc aaattgcagt
     1981 tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat ggtgacttct
     2041 acagcgcgga gaatctcact ctctccaggg gaagccgaag tttccaaaag gtcgttgatc
     2101 aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa atcaatatca
     2161 ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc
     2221 ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg
     2281 atcaccgctt ccctcatgcg aaacgatcct catcctgtc
//
