LOCUS       sequence-307085-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Diatom promoter for P. tricornutum; uLoop specific syntax; AC 
            overhangs; Lvl 0.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Windhagauer , M., Abbriano, RM., Ashworth, J., Barolo, L., 
            Jaramillo-Madrid, A.C., Pernice, M., Doblin, M.A.
  TITLE     Characterisation of novel regulatory sequences compatible with 
            modular assembly in the diatom Phaeodactylum tricornutum
  JOURNAL   Algal Research , Volume 53, March 2021
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Algal 
            Research , Volume 53, March 2021"
COMMENT     Sequence Label: pL0_AC_pSVP
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1117..1908
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1999..2587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2488..2507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
       61 tcccctgatt ctgtgggtgg aagggctcgg agttgtggta atctatgtat cctggtggtc
      121 tcgcattggt gaacttgtaa gcagagttac tggtgttaca gagcgaacct gtgtttcgtt
      181 tcgggaaaag gactttggta aaaacaggat tgtgttgttc cctcgcgttc gtgccgggat
      241 gagaatggta tttttcgtta ctcctctagt gctagactgc gctggaccgc gcacggcttg
      301 gcggccaaac ggtctttttt gtcgtgctgg cctggcccgg ccccgctacg gaccacttga
      361 aatgtgcaca gacaatgact acgagcacta cacacccaac tggataccgt agcttacaag
      421 taggcgtacc ccgtaccaac aacatgaatt tacatttaga gtcagggtaa cagggtacgg
      481 gttcgcttcg ggaggagtgg acggaaaagt gttggctccg caaatacaat taccggatgt
      541 cacttgtcct gtcagtctca atcctaatat gaagagaaaa tcaccagtga tgctaactac
      601 aaattcgatg gctgctaaaa attcatcacc atgcaaattt acaattgttt caattggcgc
      661 ggtggacctc tccggagacc tgagacgaga cgagacagcc tgagaatgga tgcgagtaat
      721 gagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
      781 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
      841 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
      901 aacgatgaca gagcgttgct gcctgtgatc accgctcctc tagagtcgac ttgcaggcat
      961 gcaagcttag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     1021 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     1081 atatctgatc aagagacagg atgaggatcg tttcgcatga gggaagcggt gatcgccgaa
     1141 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     1201 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     1261 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     1321 gaccttttgg aaacttcggc ttcccctgga gagagtgaga ttctccgcgc tgtagaagtc
     1381 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     1441 tttggagaat ggcagcgcaa tgacattctt gctggtatct tcgagccagc cacgatcgac
     1501 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     1561 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     1621 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     1681 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     1741 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     1801 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     1861 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataata atcacctaga
     1921 atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     1981 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2041 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2101 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2161 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2221 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     2281 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     2341 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     2401 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     2461 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     2521 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     2581 atggaaaaac gccagcaacg cg
//
