LOCUS       sequence-30713-e         893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 893)
  AUTHORS   Venkatachalan SP, Bushman JD, Mercado JL, Sancar F, Christopherson 
            KR, Boileau AJ
  TITLE     Optimized expression vector for ion channel studies in Xenopus 
            oocytes and mammalian cells using alfalfa mosaic virus.
  JOURNAL   Pflugers Arch. 2007 Apr . 454(1):155-63.
  PUBMED    17146677
REFERENCE   2  (bases 1 to 893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Pflugers 
            Arch. 2007 Apr . 454(1):155-63."
COMMENT     Sequence Label: pUNIV backbone
FEATURES             Location/Qualifiers
     source          1..893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      188..643
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(275..294)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     485..506
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(783..803)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
       61 tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatccg ataaggatcg atccgggctg
      121 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      181 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
      241 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
      301 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
      361 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
      421 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
      481 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
      541 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
      601 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
      661 tccttacgca tctgtgcggt atttcacacc gcatacgcgg atctgcgcag caccatggcc
      721 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
      781 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
      841 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gct
//
