LOCUS       sequence-307290-        7398 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for SARS-CoV-2 S (surface 
            glycoprotein).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7398)
  TITLE     SARS-CoV-2 protein Mammalian expression constructs, tagged, for 
            protein expression and/or purification
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pGBW-m4134343
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      199..335
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    487..558
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(563..609)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    627..675
                     /note="synthetic polyadenylation signal"
     enhancer        756..1059
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1061..1263
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1214..1234
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4137..4157
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             4506..4523
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4600..4721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4611..4630)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4665..4684
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     terminator      4836..4882
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      4912..5048
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    5093..5164
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     rep_origin      complement(5390..5978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5470..5489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(6724..6792,6793..7398,1..186)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6942..6961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
       61 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
      121 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
      181 tggtaaccga ttctaggtgc attggcgcag aaaaaaatgc ctgatgcgac gctgcgcgtc
      241 ttatactccc acatatgcca gattcagcaa cggatacggc ttccccaact tgcccacttc
      301 catacgtgtc ctccttacca gaaatttatc cttaagatcc cgaatcgttt aaactcgact
      361 ctggctctat cgaatctccg tcgtttcgag cttacgcgaa cagccgtggc gctcatttgc
      421 tcgtcgggca tcgaatctcg tcagctatcg tcagcttacc tttttggcag cgatcgcggc
      481 tcccgacatc ttggaccatt agctccacag gtatcttctt ccctctagtg gtcataacag
      541 cagcttcagc tacctctcaa ttcaaaaaac ccctcaagac ccgtttagag gccccaaggg
      601 gttatgctat caatcgttgc gttacacaca caaaaaacca acacacatcc atcttcgatg
      661 gatagcgatt ttattatcta actgctgatc gagtgtagcc agatctagta atcaattacg
      721 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      781 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      841 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
      901 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      961 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     1021 tggcagtaca tctacgtatt agtcatcgct attaccatgc tgatgcggtt ttggcagtac
     1081 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     1141 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     1201 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     1261 gctggtttag tgaaccgtca gatcagatct ttgtcgatcc taccatccac tcgacacacc
     1321 cgccagcggc cgccaccatg tctgccgacg cacagtcgtt cctgaataga gtttgtggtg
     1381 tatccgcggc ccggctaact ccatgcggaa ccgggacgag cacagatgtg gtgtaccgag
     1441 cttttgatat ctataacgac aaagtcgctg gcttcgccaa gtttcttaag accaactgtt
     1501 gcaggttcca agagaaagac gaagatgata atctcataga ctcatacttt gtggttaagc
     1561 gtcacacttt tagtaattat cagcatgagg aaacaattta taacttgctg aaggactgcc
     1621 ctgccgtagc aaaacacgac ttctttaaat tccgcatcga tggcgatatg gtcccgcata
     1681 ttagcagaca gcggttaacc aagtacacta tggcagacct ggtgtacgcc ctgcgacact
     1741 tcgacgaggg aaactgtgac acgttaaaag aaatcctcgt gacttataat tgttgcgatg
     1801 acgattattt caacaagaaa gattggtacg attttgtcga gaatcccgat atactcagag
     1861 tgtacgctaa cctgggggaa agggttcgcc aggcactatt gaagacagtc caattttgcg
     1921 acgcgatgcg gaatgccggc attgtgggtg tcctgacact tgataaccag gacctgaatg
     1981 gcaactggta tgactttgga gatttcattc agaccacgcc cgggtccgga gtacctgtcg
     2041 ttgactctta ctactcactt ctgatgccaa tcctaacctt aacccgagct ctcacagctg
     2101 agagtcacgt ggacaccgac ctcacaaaac cgtatatcaa gtgggatttg ctgaagtacg
     2161 atttcacgga agagaggtta aagctctttg atcgttattt caaatactgg gaccagactt
     2221 atcatcccaa ctgtgtgaat tgtctggacg acagatgcat acttcattgt gccaatttca
     2281 atgtactgtt tagcacagtg ttcccaccaa cttcgtttgg tcctctagtg cgcaaaatct
     2341 ttgtggatgg ggtgcccttc gttgtttcca caggctatca ctttcgtgag ctcggcgtag
     2401 ttcacaacca agatgtcaac ctgcattctt ccaggttaag ttttaaggaa cttctggtgt
     2461 atgccgcaga ccctgcgatg cacgctgcaa gcggtaattt gttgctggat aagcggacca
     2521 cgtgcttcag cgtggcggct ttaacaaata acgttgcctt ccaaaccgtc aagcctggca
     2581 acttcaataa agatttctac gatttcgccg tcagtaaagg gttttttaag gagggatcaa
     2641 gcgtagaatt gaaacatttt ttctttgctc aggacggtaa tgccgctatc tcggactacg
     2701 actactaccg gtataaccta ccaactatgt gcgacattcg gcagctgttg ttcgtggtgg
     2761 aggtcgttga taaatacttt gattgctacg atggcggatg cattaacgcc aatcaagtca
     2821 tcgtgaacaa tctggacaag agcgccggct tcccctttaa caaatgggga aaggcaaggc
     2881 tatattatga ctctatgtcc tatgaggatc aggacgctct cttcgcgtat accaagcgca
     2941 acgttatccc cacaattacc cagatgaacc tcaaatatgc aatcagcgca aagaacagag
     3001 cgaggacagt tgctggggtg agtatatgtt cgacaatgac taacaggcag tttcaccaga
     3061 agctgttgaa atctattgcc gctacacgag gggctaccgt cgtgattggc acctccaaat
     3121 tctacggagg gtggcataat atgttaaaaa ctgtgtactc cgacgtggaa aaccctcacc
     3181 tgatgggttg ggattatcct aagtgcgacc gggcgatgcc aaacatgctg agaattatgg
     3241 cgtcattagt cctggcgcga aagcacacca cgtgctgtag tctgtcacat cgcttttaca
     3301 gattggctaa tgaatgcgcc caagtgctta gcgagatggt gatgtgtggg ggttcacttt
     3361 acgtgaagcc cggaggaacg tcctcaggcg acgccacaac tgcctacgca aattcagtat
     3421 tcaacatatg tcaggccgtt acagcaaacg tgaatgctct gctgtctact gatgggaaca
     3481 agatcgccga taagtacgtc cggaacctgc aacacaggct gtacgagtgt ctgtatcgta
     3541 atcgagatgt agacaccgac ttcgtgaacg aattttacgc atacttacgg aaacatttct
     3601 ccatgatgat cctctccgac gatgcagttg tgtgctttaa ttcaacttac gcctctcagg
     3661 gcttggtggc cagtatcaaa aattttaagt cggttcttta ttatcagaat aacgtgttta
     3721 tgagcgaggc taagtgttgg accgagactg atctgaccaa aggcccgcac gagttctgtt
     3781 cccagcacac catgctggtc aagcagggtg atgactatgt ctatttacca tacccagatc
     3841 cgagtaggat cttgggagcc ggttgttttg tggatgacat tgtgaagact gacggtacac
     3901 tcatgattga gcgcttcgtc agtttggcaa tcgacgctta cccccttacc aaacatccaa
     3961 atcaggaata tgcggatgtg tttcacctgt accttcaata cataaggaaa cttcatgatg
     4021 aactaactgg acatatgctc gacatgtatt ctgtaatgct tacaaatgac aatacctcac
     4081 ggtactggga accggagttt tatgaagcca tgtacacccc acatactgtc ctgcaagaaa
     4141 acctttactt tcagtcgatg agtgacaaaa tcattcatct aaccgatgac tcctttgata
     4201 cagacgtgtt gaaggccgat ggtgcgatac tggtagattt ctgggctgaa tggtgtggcc
     4261 cgtgcaagat gatcgcaccc attctggacg agatcgccga cgagtatcaa ggaaaattaa
     4321 ctgtcgctaa gcttaatata gatcagaacc cagggacggc acctaaatac ggcatcaggg
     4381 gaatacctac cctcctgctc tttaagaatg gggaagttgc cgcgacaaaa gtgggagccc
     4441 tgtctaaggg ccagctaaaa gagttcttgg acgctaacct ggcaggttca ggctcggggc
     4501 acatgcacca tcaccatcac catagcagcg ggctcgtgcc acgcggctaa gctagcttga
     4561 ctgactgaga tacagcgtac cttcagctca cagacatgat aagatacatt gatgagtttg
     4621 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4681 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4741 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     4801 acaaatgtgg tattggccca tctctatcgg tatcgtagca taaccccttg gggcctctaa
     4861 acgggtcttg aggggttttt tgtgcccctc gggccggatt gctatctacc ggcattggcg
     4921 cagaaaaaaa tgcctgatgc gacgctgcgc gtcttatact cccacatatg ccagattcag
     4981 caacggatac ggcttcccca acttgcccac ttccatacgt gtcctcctta ccagaaattt
     5041 atccttaagg tcgtcagcta tcctgcaggc gatctctcga tttcgatcaa gacattcctt
     5101 taatggtctt ttctggacac cactaggggt cagaagtagt tcatcaaact ttcttccctc
     5161 cctaatctca ttggttacct tgggctatcg aaacttaatt aaccagtcaa gtcagctact
     5221 tggcgagatc gacttgtctg ggtttcgact acgctcagaa ttgcgtcagt caagttcgat
     5281 ctggtccttg ctattgcacc cgttctccga ttacgagttt catttaaatc atgtgagcaa
     5341 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5401 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5461 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5521 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5581 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5641 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5701 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5761 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5821 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5881 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5941 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6001 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6061 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     6121 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     6181 cagcgatctg tctatttcgt tcatccatag ttgcatttaa atttccgaac tctccaaggc
     6241 cctcgtcgga aaatcttcaa acctttcgtc cgatccatct tgcaggctac ctctcgaacg
     6301 aactatcgca agtctcttgg ccggccttgc gccttggcta ttgcttggca gcgcctatcg
     6361 ccaggtatta ctccaatccc gaatatccga gatcgggatc acccgagaga agttcaacct
     6421 acatcctcaa tcccgatcta tccgagatcc gaggaatatc gaaatcgggg cgcgcctggt
     6481 gtaccgagaa cgatcctctc agtgcgagtc tcgacgatcc atatcgttgc ttggcagtca
     6541 gccagtcgga atccagcttg ggacccagga agtccaatcg tcagatattg tactcaagcc
     6601 tggtcacggc agcgtaccga tctgtttaaa cctagatatt gatagtctga tcggtcaacg
     6661 tataatcgag tcctagcttt tgcaaacatc tatcaagaga caggatcagc aggaggcttt
     6721 cgcatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     6781 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     6841 gcgcgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     6901 cccgaagaac gctttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     6961 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     7021 ttggttgagt attcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     7081 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     7141 attggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     7201 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     7261 atgcctgtag caatggcaac aaccttgcgt aaactattaa ctggcgaact acttactcta
     7321 gcttcccggc aacagttgat agactggatg gaggcggata aagttgcagg accacttctg
     7381 cgctcggccc ttccggct
//
