LOCUS       sequence-307506-        2108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression and protien purification of mutated RUVBL1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ju D, Zhang W, Yan J, Zhao H, Li W, Wang J, Liao M, Xu Z, Wang Z, 
            Zhou G, Mei L, Hou N, Ying S, Cai T, Chen S, Xie X, Lai L, Tang C, 
            Park N, Takahashi JS, Huang N, Qi X, Zhang EE
  TITLE     Chemical perturbations reveal that RUVBL2 regulates the circadian 
            phase in mammals.
  JOURNAL   Sci Transl Med. 2020 May 6;12(542). pii: 12/542/eaba0769. doi: 
            10.1126/scitranslmed.aba0769.
  PUBMED    32376767
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Transl 
            Med."; date: "2020-05-6"; volume: "12(542). pii: 12/542/eaba0769. 
            doi"; pages: " 10.1126/scitranslmed.aba0769"
COMMENT     Sequence Label: pET-DEUT RUVBL1 Delete
FEATURES             Location/Qualifiers
     source          1..2108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     185..204
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(224..301)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     507..526
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        614..632
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    633..657
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             672..694
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             713..730
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             749..772
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     promoter        1904..1922
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1923..1947
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2056..2100
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gcgtgcaggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
       61 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
      121 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
      181 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
      241 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
      301 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
      361 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
      421 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      481 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      541 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatcgatctc
      601 gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa caattcccct
      661 ctagaaataa ttttgtttaa ctttaagaag gagatatacc atgggcagca gccatcacca
      721 tcatcaccac agccaggatc cgaattcgct ggaagttctg ttccaggggc cctcgaagat
      781 tgaggaggtg aagagcacta cgaagacgca gcgcatcgcc tcccacagcc acgtgaaagg
      841 gctggggctg gacgagagcg gcttggccaa gcaggcggcc tcagggcttg tgggccagga
      901 gaacgcgcga gaggcatgtg gcgtcatagt agaattaatc aaaagcaaga aaatggctgg
      961 aagagctgtc ttgttggcag gacctcctgg aactggcaag acagctctgg ctctggctat
     1021 tgctcaggag ctgggtagta aggtcccctt ctgcccaatg gtggggagtg aagtttactc
     1081 aactgagatc aagaagacag aggtgctgat ggagaacttc cgcagggcca ttgggctgcg
     1141 aataaaggag ggtccaccag gtatcatcca agatgtgacc ttgcatgact tggatgtggc
     1201 taatgcgcgg ccccaggggg gacaagatat cctgtccatg atgggccagc taatgaagcc
     1261 aaagaagaca gaaatcacag acaaacttcg aggggagatt aataaggtgg tgaacaagta
     1321 catcgaccag ggcattgctg agctggtccc gggtgtgctg tttgttgatg aggtccacat
     1381 gctggacatt gagtgcttca cctacctgca ccgcgccctg gagtcttcta tcgctcccat
     1441 cgtcatcttt gcatccaacc gaggcaactg tgtcatcaga ggcactgagg acatcacatc
     1501 ccctcacggc atccctcttg accttctgga ccgagtgatg ataatccgga ccatgctgta
     1561 tactccacag gaaatgaaac agatcattaa aatccgtgcc cagacggaag gaatcaacat
     1621 cagtgaggag gcactgaacc acctggggga gattggcacc aagaccacac tgaggtactc
     1681 agtgcagctg ctgaccccgg ccaacttgct tgctaaaatc aacgggaagg acagcattga
     1741 gaaagagcat gtcgaagaga tcagtgaact tttctatgat gccaagtcct ccgccaaaat
     1801 cctggctgac cagcaggata agtacatgaa gtaagcttgc ggccgcataa tgcttaagtc
     1861 gaacagaaag taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata
     1921 ggggaattgt gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga
     1981 gatatacata tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt
     2041 accctcgagt ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg
     2101 tctactag
//
