LOCUS       sequence-307778-        1233 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast codon 
            optimized mRuby2 under KanMX selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1233)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1233)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP1931
FEATURES             Location/Qualifiers
     source          1..1233
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(91..801)
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     promoter        complement(871..889)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(872..889)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(970..989)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atttgtatac acttattttt tttataactt atttaataat aaaaatcata aatcataaga
       61 aattcgctta tttagaagtg gcgcgcctca cttgtataat tcatccatac caccacccaa
      121 accagcaaat ttagcaacag cgtgttctct ttgaacgacg aacatttcgt tgtcggattc
      181 ttctaatctt tccaatctgt ggtcaacagc atgaatacct ggcatcttaa tgttaccgac
      241 ggtctttttg gatctgtagg tagtaacaaa agaacaagac aaatgaccac caccgtcaac
      301 tttcaaagcc atatgagtat aacctctcaa accaccatca gctggataca tcatttcggt
      361 gttaggttcc caaccttttg tctttttttg catgactgga ccgttagatg gaaagttaac
      421 acctctaact tgaacgtggt aaaccaagca accatcttcc aaagaagtat cttgcataac
      481 ggtaacaaca ccaccatctt cgtatctagt aactctttcc caggtgaaac cttctggaaa
      541 agattgtttg aagaagtctg ggataccctt tgggtactta atgaaagttc tggaaccgta
      601 catgaaagag gtagccaaaa tatcaaaagc gaatggcaat ggaccacctt caatgacttt
      661 gattctcatg gtttgagtac ccatataagg attaccttca ccttcaccag tacacttgaa
      721 ttgatgaccg ttaacagaac cttccataac gaccttcatt ctcatgtttt ctttgatcaa
      781 ttcttcaccc ttggaaacca tgttaattaa accagcaccg tcaccgatcc gtcgacctgc
      841 agcgtacgaa gcttcagctg gcggccgcgt tctatagtgt cacctaaatc gtatgtgtat
      901 gatacataag gttatgtatt aattgtagcc gcgttctaac gacaatatgt ccatatggtg
      961 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     1021 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     1081 gaccgtctcc gggagctgca tgkgtcarag gttttcaccg tcatcmccga aacgcscgar
     1141 acgaaagggc ctcgtgawac gcctattttt awagggtaaa ggcatgaaaa aaaagggttt
     1201 cttaracgtc aggggggmmt ttttcgggga aat
//
