LOCUS       sequence-307780-        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast tagging vector to create gene fusion with yeast codon 
            optimized tdKOkappa under KanMX selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  TITLE     Stelling Lab Plasmids
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FRP1864
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(74..271)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(277..1086)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1000..1019
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 ctagtggatc tgatatcatc gatgaattcg agctcgttta aactggatgg cggcgttagt
       61 atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt
      121 tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca
      181 cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt
      241 tcttgaaaac aagaatcttt ttattgtcag tactgattag aaaaactcat cgagcatcaa
      301 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
      361 ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat cctggtatcg
      421 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
      481 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
      541 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
      601 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
      661 atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca ggaacactgc
      721 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
      781 tttgccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
      841 gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct catctgtaac
      901 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
      961 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     1021 atataaatca gcatccatgt tggaatttaa tcgcggcctc gaaacgtgag tcttttcctt
     1081 acccatgggt gtttatgttc ggatgkga
//
