LOCUS       sequence-307846-        7791 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mEGFP fused to human linker histone H1.4 without 
            c-terminal tail.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7791)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 7791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pMTTH+mEGFP-H1.4-Delta-CTD
FEATURES             Location/Qualifiers
     source          1..7791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(129..134,135..150,151..157)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    165..181
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     170..192
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             251..1351
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1208..1217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1325..1348
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1400..1411
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1424..1444
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(1448..1450,1451..1453,1454..2164)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1493..1514)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1754..1773)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2101..2122
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2531..2551
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2552..2569
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2582..2599)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2582..2598)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2591..2613)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2777..2794)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2947..3033
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3125..3152
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3171..3262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3263..3331,3332..4123)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3481..3500)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(4165..4678)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4360..4381)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4572..4591
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      4789..5377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5278..5297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5531..5548
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5654..5673)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5814..5836
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(5830..6021)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        6517..6594
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             6595..7677
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(6614..6633)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac
       61 tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa
      121 atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
      181 atttcacaca ggaaacagcc agtccgttta ggtgttttca cgagcacttc accaacaagg
      241 accatagatt atgaaaactg aagaaggtaa actggtaatc tggattaacg gcgataaagg
      301 ctataacggt ctcgctgaag tcggtaagaa attcgagaaa gataccggaa ttaaagtcac
      361 cgttgagcat ccggataaac tggaagagaa attcccacag gttgcggcaa ctggcgatgg
      421 ccctgacatt atcttctggg cacacgaccg ctttggtggc tacgctcaat ctggcctgtt
      481 ggctgaaatc accccggaca aagcgttcca ggacaagctg tatccgttta cctgggatgc
      541 cgtacgttac aacggcaagc tgattgctta cccgatcgct gttgaagcgt tatcgctgat
      601 ttataacaaa gatctgctgc cgaacccgcc aaaaacctgg gaagagatcc cggcgctgga
      661 taaagaactg aaagcgaaag gtaagagcgc gctgatgttc aacctgcaag aaccgtactt
      721 cacctggccg ctgattgctg ctgacggggg ttatgcgttc aagtatgaaa acggcaagta
      781 cgacattaaa gacgtgggcg tggataacgc tggcgcgaaa gcgggtctga ccttcctggt
      841 tgacctgatt aaaaacaaac acatgaatgc agacaccgat tactccatcg cagaagctgc
      901 ctttaataaa ggcgaaacag cgatgaccat caacggcccg tgggcatggt ccaacatcga
      961 caccagcaaa gtgaattatg gtgtaacggt actgccgacc ttcaagggtc aaccatccaa
     1021 accgttcgtt ggcgtgctga gcgcaggtat taacgccgcc agtccgaaca aagagctggc
     1081 aaaagagttc ctcgaaaact atctgctgac tgatgaaggt ctggaagcgg ttaataaaga
     1141 caaaccgctg ggtgccgtag cgctgaagtc ttacgaggaa gagttggcga aagatccacg
     1201 tattgccgcc accatggaaa acgcccagaa aggtgaaatc atgccgaaca tcccgcagat
     1261 gtccgctttc tggtatgccg tgcgtactgc ggtgatcaac gccgccagcg gtcgtcagac
     1321 tgtcgatgaa gccctgaaag acgcgcagac taattcgagc tcgaacaaca acaacaataa
     1381 caataacaac aacctcggga tcgagggaag gatttcagaa tttgaaaacc tgtattttca
     1441 gggccatatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccta tcctggtcga
     1501 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1561 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc cagtgccgtg
     1621 gcctaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct acccagacca
     1681 catgaagcag cacgacttct tcaagtccgc catgccggaa ggctacgtcc aggagcgcac
     1741 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1801 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1861 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1921 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1981 gctcgccgac cactaccagc agaacacccc tatcggcgac ggcccagtgc tgctgccgga
     2041 caaccactac ctgagcaccc agtccaaact gagcaaagac cctaacgaga agcgcgatca
     2101 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     2161 caagggaggc agtggtggat catcagaaac tgcacctgca gcgcctgctg ctccggcacc
     2221 tgccgagaaa actcccgtga agaagaaggc tagaaagtct gcaggtgcgg ccaagcgcaa
     2281 agcgtctggg cccccggtgt ccgagctcat tactaaagct gttgccgcct ccaaggagcg
     2341 cagcggcgta tctttggccg ctctcaagaa agcgctggca gccgctggct atgacgtgga
     2401 gaagaacaac agccgcatca agctgggtct caagagcctg gtgagcaagg gcaccctggt
     2461 gcagaccaag ggcaccggcg cgtcgggttc cttcaaactc aacaagaagg cggccggctc
     2521 gagcggatcc gaaaacctgt attttcaggg ccatcatcat catcatcact gaaagcttgg
     2581 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     2641 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     2701 gcccttccca acagttgcgc agcctgaatg gcgaatggca gcttggctgt tttggcggat
     2761 gagataagat tttcagcctg atacagatta aatcagaacg cagaagcggt ctgataaaac
     2821 agaatttgcc tggcggcagt agcgcggtgg tcccacctga ccccatgccg aactcagaag
     2881 tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc
     2941 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     3001 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
     3061 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
     3121 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tttgtttatt
     3181 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     3241 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     3301 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     3361 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     3421 gatccttgag agttttcgcc ccgaagaacg tttcccaatg atgagcactt ttaaagttct
     3481 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     3541 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     3601 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     3661 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     3721 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     3781 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     3841 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     3901 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     3961 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4021 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     4081 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     4141 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg
     4201 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta
     4261 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     4321 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     4381 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     4441 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta
     4501 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     4561 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     4621 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtaa
     4681 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     4741 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     4801 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     4861 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     4921 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     4981 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     5041 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     5101 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     5161 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     5221 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     5281 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     5341 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     5401 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     5461 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     5521 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     5581 ccttacgcat ctgtgcggta tttcacaccg catgtggtgc actctcagta caccgcatgt
     5641 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
     5701 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
     5761 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     5821 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
     5881 ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
     5941 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     6001 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
     6061 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
     6121 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
     6181 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
     6241 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
     6301 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
     6361 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
     6421 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
     6481 cacccgtggc caggacccaa cgctgcccga aattccgaca ccatcgaatg gtgcaaaacc
     6541 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
     6601 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
     6661 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     6721 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
     6781 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     6841 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     6901 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     6961 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     7021 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     7081 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     7141 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     7201 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     7261 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     7321 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     7381 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
     7441 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     7501 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     7561 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     7621 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7681 caacgcaatt aatgtaagtt agctcactca ttaggcacaa ttctcatgtt tgacagctta
     7741 tcatcgactg cacggtgcac caatgcttct ggcgtcaggc agccatcgga a
//
