LOCUS       sequence-307856-        9606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of synthetic protein composed of eGFP and 5 repeats of 
            BRD4 bromodomain #1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9606)
  AUTHORS   Gibson BA, Doolittle LK, Schneider MWG, Jensen LE, Gamarra N, Henry 
            L, Gerlich DW, Redding S, Rosen MK
  TITLE     Organization of Chromatin by Intrinsic and Regulated Phase 
            Separation.
  JOURNAL   Cell. 2019 Oct 3;179(2):470-484.e21. doi: 
            10.1016/j.cell.2019.08.037. Epub 2019 Sep 19.
  PUBMED    31543265
REFERENCE   2  (bases 1 to 9606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2019.08.037"; journalName: "Cell"; date: "2019-10-3-
            3"; volume: "179"; issue: "2"; pages: "470-484.e21"
COMMENT     Sequence Label: pMTTH+eGFP-bromo5
FEATURES             Location/Qualifiers
     source          1..9606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(113..118,119..134,135..141)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    149..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     154..176
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     CDS             235..1335
                     /codon_start=1
                     /gene="malE (mutated)"
                     /product="maltose binding protein from E. coli"
                     /label=MBP
                     /note="This version of the gene does not encode a signal 
                     sequence, so MBP will remain in the cytosol."
                     /translation="MKTEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLE
                     EKFPQVAATGDGPDIIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKL
                     IAYPIAVEALSLIYNKDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIA
                     ADGGYAFKYENGKYDIKDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGE
                     TAMTINGPWAWSNIDTSKVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFL
                     ENYLLTDEGLEAVNKDKPLGAVALKSYEEELAKDPRIAATMENAQKGEIMPNIPQMSAF
                     WYAVRTAVINAASGRQTVDEALKDAQT"
     regulatory      1192..1201
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1309..1332
                     /label=MBP-F
                     /note="Maltose binding protein, forward primer"
     CDS             1384..1395
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             1408..1428
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(1438..1440,1441..1443,1444..2154)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1438..1440,1441..1443,1444..2154)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1483..1504)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1744..1763)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2091..2112
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4330..4350
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             4351..4368
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4381..4398)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4381..4397)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4390..4412)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4576..4593)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      4746..4832
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4924..4951
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        4970..5061
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5062..5130,5131..5922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5280..5299)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(5964..6477)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6159..6180)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6371..6390
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      6588..7176
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7077..7096
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7330..7347
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7453..7472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7613..7635
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7629..7820)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     promoter        8316..8393
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8394..9476
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8413..8432)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt tctggataat
       61 gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc tgttgacaat
      121 taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca cacaggaaac
      181 agccagtccg tttaggtgtt ttcacgagca cttcaccaac aaggaccata gattatgaaa
      241 actgaagaag gtaaactggt aatctggatt aacggcgata aaggctataa cggtctcgct
      301 gaagtcggta agaaattcga gaaagatacc ggaattaaag tcaccgttga gcatccggat
      361 aaactggaag agaaattccc acaggttgcg gcaactggcg atggccctga cattatcttc
      421 tgggcacacg accgctttgg tggctacgct caatctggcc tgttggctga aatcaccccg
      481 gacaaagcgt tccaggacaa gctgtatccg tttacctggg atgccgtacg ttacaacggc
      541 aagctgattg cttacccgat cgctgttgaa gcgttatcgc tgatttataa caaagatctg
      601 ctgccgaacc cgccaaaaac ctgggaagag atcccggcgc tggataaaga actgaaagcg
      661 aaaggtaaga gcgcgctgat gttcaacctg caagaaccgt acttcacctg gccgctgatt
      721 gctgctgacg ggggttatgc gttcaagtat gaaaacggca agtacgacat taaagacgtg
      781 ggcgtggata acgctggcgc gaaagcgggt ctgaccttcc tggttgacct gattaaaaac
      841 aaacacatga atgcagacac cgattactcc atcgcagaag ctgcctttaa taaaggcgaa
      901 acagcgatga ccatcaacgg cccgtgggca tggtccaaca tcgacaccag caaagtgaat
      961 tatggtgtaa cggtactgcc gaccttcaag ggtcaaccat ccaaaccgtt cgttggcgtg
     1021 ctgagcgcag gtattaacgc cgccagtccg aacaaagagc tggcaaaaga gttcctcgaa
     1081 aactatctgc tgactgatga aggtctggaa gcggttaata aagacaaacc gctgggtgcc
     1141 gtagcgctga agtcttacga ggaagagttg gcgaaagatc cacgtattgc cgccaccatg
     1201 gaaaacgccc agaaaggtga aatcatgccg aacatcccgc agatgtccgc tttctggtat
     1261 gccgtgcgta ctgcggtgat caacgccgcc agcggtcgtc agactgtcga tgaagccctg
     1321 aaagacgcgc agactaattc gagctcgaac aacaacaaca ataacaataa caacaacctc
     1381 gggatcgagg gaaggatttc agaatttgaa aacctgtatt ttcagggcca tatggccatg
     1441 gtgagcaagg gcgaggagct gttcaccggg gtggtgccta tcctggtcga gctggacggc
     1501 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     1561 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc cagtgccgtg gcctaccctc
     1621 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct acccagacca catgaagcag
     1681 cacgacttct tcaagtccgc catgccggaa ggctacgtcc aggagcgcac catcttcttc
     1741 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1801 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1861 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     1921 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     1981 cactaccagc agaacacccc tatcggcgac ggcccagtgc tgctgccgga caaccactac
     2041 ctgagcaccc agtccgccct gagcaaagac cctaacgaga agcgcgatca catggtcctg
     2101 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggtggc
     2161 agcggcgggt cgggcgggag cggcggcagt ggaggaagtg ttgcctcgac taatcctcca
     2221 ccgccggaga ccagtaaccc aaacaagcct aaacgtcaaa cgaaccaact ccaatatctg
     2281 ttgcgtgttg ttttgaaaac attgtggaaa caccaatttg catggccttt tcaacaaccg
     2341 gttgacgcgg tgaagttaaa ccttccggat tattacaaaa ttatcaaaac tccgatggat
     2401 atggggacta tcaaaaaacg gttggagaac aactactact ggaatgccca agagtgtatt
     2461 caagatttca acaccatgtt cacaaactgt tatatctaca acaaaccagg agacgatatc
     2521 gttctgatgg ccgaggccct tgagaaatta tttttacaga aaatcaacga actcccgaca
     2581 gaggagggcg ggtccggtgg aagtggcggt agtggtggga gtggtggatc agccgccagc
     2641 acaaacccac ctccgcctga aacgtccaac ccgaacaaac cgaaacggca gactaaccag
     2701 ctgcagtact tacttcgggt agtattgaag acactgtgga agcatcagtt cgcctggccg
     2761 ttccaacagc cagtagatgc agttaaactt aatttacctg attattataa aatcattaaa
     2821 acacctatgg atatgggaac tattaagaaa cgcctggaga ataactatta ttggaatgct
     2881 caagaatgta tccaggattt caataccatg tttacgaact gttacattta caataaacct
     2941 ggtgatgata tcgtgcttat ggcagaagca ctggaaaagc tgtttttgca aaaaattaat
     3001 gagctgccta ctgaggaagg gggaagcggg ggctcggggg ggtcaggcgg ttctggagga
     3061 tctgccgcat ccactaaccc gcctccgcca gagacgagta atccgaacaa gccgaagcgc
     3121 caaacgaatc aattacagta tcttcttcgt gtcgtgttaa aaacgctttg gaagcaccag
     3181 tttgcgtggc cattccagca gcctgtggat gcggttaaat taaatctccc ggactattac
     3241 aagatcatca aaacccctat ggatatgggc acgatcaaga agcgcctcga aaacaattac
     3301 tattggaatg cgcaagaatg catccaggac tttaatacga tgtttactaa ctgctatatt
     3361 tataataaac cgggcgatga tattgtattg atggcggaag ctcttgaaaa attgtttctt
     3421 cagaagatca atgaactccc taccgaagag ggaggttcgg gagggagcgg tggatctgga
     3481 ggctccggag ggtctgctgc gtctaccaat ccgccaccac cggaaactag caatcctaac
     3541 aaaccaaaac gtcagaccaa tcaactgcaa tacctcttac gcgttgtgct taaaaccctc
     3601 tggaaacacc agtttgcctg gcctttccag caaccagtgg acgccgtgaa gttgaacctg
     3661 ccagactact ataagatcat taagacgcca atggatatgg gtacaattaa gaagcggttg
     3721 gaaaacaact attattggaa tgcacaggaa tgtatccaag acttcaatac tatgtttaca
     3781 aattgctaca tctataataa gccgggagat gacattgttt taatggctga ggcattagaa
     3841 aagttgttct tacaaaagat taatgaattg cctacggaag aaggtggctc tgggggctca
     3901 ggtggtagtg ggggttccgg gggctccgca gcctcgacca accctcctcc acctgagacc
     3961 tctaatccaa ataaacctaa gcggcaaacc aatcagctcc agtacctgct tcgcgtagta
     4021 ctgaagacct tatggaaaca tcaattcgct tggccgtttc aacagcctgt tgatgctgtt
     4081 aagctgaact tgcctgatta ctacaagatt atcaagaccc cgatggacat ggggacgatt
     4141 aaaaagcggc ttgaaaataa ttattattgg aacgcccagg agtgcattca ggattttaac
     4201 acgatgttca ccaattgtta tatttacaac aagccagggg acgacatcgt actcatggca
     4261 gaggcacttg agaaactttt cctccagaaa attaacgagt taccaaccga ggagggctcg
     4321 agcggatccg aaaacctgta ttttcagggc catcatcatc atcatcactg aaagcttggc
     4381 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4441 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4501 cccttcccaa cagttgcgca gcctgaatgg cgaatggcag cttggctgtt ttggcggatg
     4561 agataagatt ttcagcctga tacagattaa atcagaacgc agaagcggtc tgataaaaca
     4621 gaatttgcct ggcggcagta gcgcggtggt cccacctgac cccatgccga actcagaagt
     4681 gaaacgccgt agcgccgatg gtagtgtggg gtctccccat gcgagagtag ggaactgcca
     4741 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
     4801 tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg aacgttgcga
     4861 agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg catcaaatta
     4921 agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt ttgtttattt
     4981 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     5041 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     5101 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     5161 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     5221 atccttgaga gttttcgccc cgaagaacgt ttcccaatga tgagcacttt taaagttctg
     5281 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     5341 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     5401 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5461 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5521 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5581 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5641 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5701 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5761 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5821 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5881 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5941 tcatatatac tttagattga tttaccccgg ttgataatca gaaaagcccc aaaaacagga
     6001 agattgtata agcaaatatt taaattgtaa acgttaatat tttgttaaaa ttcgcgttaa
     6061 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     6121 aatcaaaaga atagcccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     6181 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     6241 cactacgtga accatcaccc aaatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     6301 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     6361 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     6421 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtaaa
     6481 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     6541 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     6601 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     6661 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     6721 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     6781 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     6841 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     6901 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     6961 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     7021 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     7081 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     7141 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     7201 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     7261 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     7321 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc
     7381 cttacgcatc tgtgcggtat ttcacaccgc atgtggtgca ctctcagtac accgcatgtg
     7441 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta
     7501 tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc
     7561 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     7621 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc
     7681 tcatcagcgt ggtcgtgcag cgattcacag atgtctgcct gttcatccgc gtccagctcg
     7741 ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg
     7801 gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta
     7861 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     7921 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     7981 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt
     8041 agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc
     8101 gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca
     8161 gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa
     8221 ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc
     8281 acccgtggcc aggacccaac gctgcccgaa attccgacac catcgaatgg tgcaaaacct
     8341 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac
     8401 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg
     8461 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg
     8521 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc
     8581 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga
     8641 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg
     8701 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga
     8761 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg
     8821 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc
     8881 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg
     8941 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata
     9001 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca
     9061 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc
     9121 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc
     9181 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata
     9241 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc
     9301 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac
     9361 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg
     9421 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     9481 aacgcaatta atgtaagtta gctcactcat taggcacaat tctcatgttt gacagcttat
     9541 catcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat
     9601 ggctgt
//
