LOCUS       sequence-308075-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of proteins with a C-terminal thermostable GFP (TGP) tag 
            in Escherichia coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D
  TITLE     An improved fluorescent tag and its nanobodies for membrane protein 
            expression, stability assay, and purification.
  JOURNAL   Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z.
  PUBMED    33303987
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-12-10"; volume: "3(1):753. doi"; pages: " 
            10.1038/s42003-020-01478-z"
COMMENT     Sequence Label: pETSG
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             73..96
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             790..813
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatatacc atgggatcca agcttagcgg ccgcgaattc
       61 ctcgagggta ccctggaagt gctgtttcag ggcccgggcg gtagtggagg ttccgcgagt
      121 gtcattaagc cggagatgaa aattaagttg cgtatggagg gggccgtgaa tgggcacaaa
      181 ttcgttattg aaggggaggg gattggaaag ccgtatgagg gtacgcaaac cttggactta
      241 acagtagaag aaggggcacc attgcctttt tcttatgata ttttgactcc ggcgttccaa
      301 tacgggaacc gtgctttcac aaagtatccc gaagatatcc ctgactattt caagcaagca
      361 tttcccgagg gttacagttg ggagagaagt atgacctatg aggatcaagg catatgtatt
      421 gctacctctg acattactat ggagggagac tgcttcttct atgagattcg tttcgatgga
      481 acaaactttc ctcctaacgg gccagtgatg cagaaaaaga cgttgaagtg ggaaccgtcc
      541 acagaaaaaa tgtatgtcga ggacggcgtt ctaaagggag acgtggaaat ggccttgcta
      601 ttggaagggg gtggacacta tcgttgtgat ttcaaaacga cctacaaggc caaaaaagat
      661 gtgagactac cggacgccca cgaggttgac catcgtattg aaattttaag tcacgacaag
      721 gactataata aagtaagact gtacgaacac gcggaagcac gttacagcgg aggtggaagt
      781 ggtggtggcc atcatcatca ccaccaccac cattgagatc cggctgctaa caaagcccga
      841 aaggaagctg agttggctgc tgccaccgct g
//
