LOCUS       sequence-308077-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of proteins with a C-terminal thermostable GFP (TGP) tag 
            in Saccharomyces cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D
  TITLE     An improved fluorescent tag and its nanobodies for membrane protein 
            expression, stability assay, and purification.
  JOURNAL   Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z.
  PUBMED    33303987
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2020-12-10"; volume: "3(1):753. doi"; pages: " 
            10.1038/s42003-020-01478-z"
COMMENT     Sequence Label: pYTSG
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..40
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     78..97
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        78..96
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             155..178
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             872..892
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     primer_bind     complement(933..951)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
ORIGIN
        1 tcaacaaaaa attgttaata tacctctata ctttaacgtc aaggagaaaa aaccccggat
       61 cggactacta gcagctgtaa tacgactcac tatagggaat atttaaaaaa tgtctggatc
      121 caagcttagc ggccgcgaat tcctcgaggg taccctggaa gtgctgtttc agggcccggg
      181 cggtagtgga ggttccgcga gtgtcattaa gccggagatg aaaattaagt tgcgtatgga
      241 gggggccgtg aatgggcaca aattcgttat tgaaggggag gggattggaa agccgtatga
      301 gggtacgcaa accttggact taacagtaga agaaggggca ccattgcctt tttcttatga
      361 tattttgact ccggcgttcc aatacgggaa ccgtgctttc acaaagtatc ccgaagatat
      421 ccctgactat ttcaagcaag catttcccga gggttacagt tgggagagaa gtatgaccta
      481 tgaggatcaa ggcatatgta ttgctacctc tgacattact atggagggag actgcttctt
      541 ctatgagatt cgtttcgatg gaacaaactt tcctcctaac gggccagtga tgcagaaaaa
      601 gacgttgaag tgggaaccgt ccacagaaaa aatgtatgtc gaggacggcg ttctaaaggg
      661 agacgtggaa atggccttgc tattggaagg gggtggacac tatcgttgtg atttcaaaac
      721 gacctacaag gccaaaaaag atgtgagact accggacgcc cacgaggttg accatcgtat
      781 tgaaatttta agtcacgaca aggactataa taaagtaaga ctgtacgaac acgcggaagc
      841 acgttacagc ggaggtggaa gtggtggtgg ccatcaccat caccatcacc attagcggcc
      901 gctctagagg gccgcatcat gtaattagtt atgtcacgct tacattcacg ccctcccccc
      961 acatccgctc taaccg
//
