LOCUS       sequence-30809-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Ayed A, Mulder FA, Yi GS, Lu Y, Kay LE, Arrowsmith CH
  TITLE     Latent and active p53 are identical in conformation.
  JOURNAL   Nat Struct Biol. 2001 Sep . 8(9):756-60.
  PUBMED    11524676
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol. 2001 Sep . 8(9):756-60."
COMMENT     Sequence Label: human p53-(82-393)
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             57..74
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             84..101
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 aattcctctg aatattttgt ttaactttaa gaaggagata taccatgggc agcagccatc
       61 atcatcatca tcacagcagc ggcctggtgc cgcgcggcag ccatatgccg gcggcccctg
      121 caccagcccc ctcctggccc ctgtcatctt ctgtcccttc ccagaaaacc taccagggca
      181 gctacggttt ccgtctgggc ttcttgcatt ctgggacagc caagtctgtg acttgcacgt
      241 actcccctgc cctcaacaag atgttttgcc aactggccaa gacctgccct gtgcagctgt
      301 gggttgattc cacacccccg cccggcaccc gcgtccgcgc catggccatc tacaagcagt
      361 cacagcacat gacggaggtt gtgaggcgct gcccccacca tgagcgctgc tcagatagcg
      421 atggtctggc ccctcctcag catcttatcc gagtggaagg aaatttgcgt gtggagtatt
      481 tggatgacag aaacactttt cgacatagtg tggtggtgcc ctatgagccg cctgaggttg
      541 gctctgactg taccaccatc cactacaact acatgtgtaa cagttcctgc atgggcggca
      601 tgaaccggag gcccatcctc accatcatca cactggaaga ctccagtggt aatctactgg
      661 gacggaacag ctttgaggtg cgtgtttgtg cctgtcctgg gagagaccgg cgcacagagg
      721 aagagaatct ccgcaagaaa ggggagcctc accacgagct gcccccaggg agcactaagc
      781 gagcactgcc caacaacacc agctcctctc cccagccaaa gaaaaaacca ctggatggag
      841 aatatttcac ccttcagatc cgtgggcgtg agcgcttcga gatgttccga gagctgaatg
      901 aggccttgga actcaaggat gcccaggctg ggaa
//
