LOCUS       sequence-308133-        9730 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive lentiviral expression of human TMPRSS4..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Chavez Lab
  TITLE     Coronavirus host receptors and proteases
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLEX307-TMPRSS4-puro
FEATURES             Location/Qualifiers
     source          1..9730
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..1274
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          327..1265
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1222..1242
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1294..1318
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2209..2220)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(2639..2663)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2671..2712
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2763..3351
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2816..2836)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3234..3245)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3423..3656
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3734..3855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3771..3790)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3825..3844
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3895..4030
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4050..4069)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4051..4069)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4079..4096)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4079..4095)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4088..4110)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4237..4692
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4324..4343)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4534..4555
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4718..4822
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4823..4891,4892..5683)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5041..5060)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5854..6442
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6343..6362
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6596..6613
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6730..6751
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6766..6771,6772..6789,6790..6796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6804..6820
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6809..6831
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6828..6844
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6828..6844
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6863..6883
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6865..6883
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6911..7137
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7138..7318
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7365..7490
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7983..8216
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8401..8445
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8676..8886
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8698..8894
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8745..8880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8926..9528
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8926..8945)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9422..9442
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9571..9688
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctcgaggcct gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct
       61 aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag
      121 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg
      181 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      241 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      301 gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc
      361 tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga
      421 ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag
      481 gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc
      541 gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa
      601 atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc
      661 aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt
      721 cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg
      781 ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc
      841 cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc
      901 ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg
      961 gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact
     1021 ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt
     1081 cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg
     1141 agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg
     1201 agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat
     1261 ttcaggtgtc gtgaggctag catcgattga tcaacaagtt tgtacaaaaa agcaggctac
     1321 aaaatgttac aggatcctga cagtgatcaa cctctgaaca gcctcgatgt caaacccctg
     1381 cgcaaacccc gtatccccat ggagaccttc agaaaggtgg ggatccccat catcatagca
     1441 ctactgagcc tggcgagtat catcattgtg gttgtcctca tcaaggtgat tctggataaa
     1501 tactacttcc tctgcgggca gcctctccac ttcatcccga ggaagcagct gtgtgacgga
     1561 gagctggact gtcccttggg ggaggacgag gagcactgtg tcaagagctt ccccgaaggg
     1621 cctgcagtgg cagtccgcct ctccaaggac cgatccacac tgcaggtgct ggactcggcc
     1681 acagggaact ggttctctgc ctgtttcgac aacttcacag aagctctcgc tgagacagcc
     1741 tgtaggcaga tgggctacag cagcaaaccc actttcagag ctgtggagat tggcccagac
     1801 caggatctgg atgttgttga aatcacagaa aacagccagg agcttcgcat gcggaactca
     1861 agtgggccct gtctctcagg ctccctggtc tccctgcact gtcttgcctg tgggaagagc
     1921 ctgaagaccc cccgtgtggt gggtggggag gaggcctctg tggattcttg gccttggcag
     1981 gtcagcatcc agtacgacaa acagcacgtc tgtggaggga gcatcctgga cccccactgg
     2041 gtcctcacgg cagcccactg cttcaggaaa cataccgatg tgttcaactg gaaggtgcgg
     2101 gcaggctcag acaaactggg cagcttccca tccctggctg tggccaagat catcatcatt
     2161 gaattcaacc ccatgtaccc caaagacaat gacatcgccc tcatgaagct gcagttccca
     2221 ctcactttct caggcacagt caggcccatc tgtctgccct tctttgatga ggagctcact
     2281 ccagccaccc cactctggat cattggatgg ggctttacga agcagaatgg agggaagatg
     2341 tctgacatac tgctgcaggc gtcagtccag gtcattgaca gcacacggtg caatgcagac
     2401 gatgcgtacc agggggaagt caccgagaag atgatgtgtg caggcatccc ggaagggggt
     2461 gtggacacct gccagggtga cagtggtggg cccctgatgt accaatctga ccagtggcat
     2521 gtggtgggca tcgttagctg gggctatggc tgcgggggcc cgagcacccc aggagtatac
     2581 accaaggtct cagcctatct caactggatc tacaatgtct ggaaggctga gctgtaaaac
     2641 ccagctttct tgtacaaagt ggttgatatc ggtaagccta tccctaaccc tctcctcggt
     2701 ctcgattcta cgtagtaatg aactagtacc ggttaagtcg acaatcaacg cgttaagtcg
     2761 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     2821 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     2881 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     2941 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     3001 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     3061 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     3121 tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc
     3181 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     3241 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     3301 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt
     3361 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     3421 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     3481 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     3541 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     3601 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtac
     3661 gtatagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     3721 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     3781 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     3841 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     3901 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     3961 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     4021 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     4081 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4141 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4201 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4261 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4321 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4381 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4441 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4501 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4561 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4621 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4681 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4741 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4801 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4861 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4921 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4981 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5041 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5101 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5161 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5221 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5281 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5341 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5401 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5461 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5521 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5581 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5641 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5701 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5761 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5821 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5881 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5941 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6001 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6061 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6121 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6181 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6241 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6301 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6361 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6421 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6481 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6541 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6601 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6661 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6721 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6781 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6841 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     6901 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     6961 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     7021 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     7081 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     7141 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7201 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7261 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7321 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     7381 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     7441 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     7501 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     7561 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     7621 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     7681 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     7741 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     7801 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     7861 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     7921 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     7981 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8041 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8101 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     8161 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     8221 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     8281 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     8341 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     8401 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     8461 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     8521 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     8581 ttatcgtttc agacccacct cccaaccccg aggggaccca tgcattgcat ctcaattagt
     8641 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     8701 gtctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     8761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     8821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     8881 taggcttttg caaaaagctt tctagaggta ccaccggagc ttaccatgac cgagtacaag
     8941 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     9001 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     9061 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     9121 gtcgcggacg acggcgccgc cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     9181 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     9241 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     9301 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     9361 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     9421 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     9481 gaaggaccgc gcacctggtg catgacccgc aagcccggtg ccagttaacg aattctagat
     9541 cttgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     9601 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     9661 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     9721 ttggcgccgg
//
