LOCUS       sequence-30819-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Sheng Y, Laister RC, Lemak A, Wu B, Tai E, Duan S, Lukin J, 
            Sunnerhagen M, Srisailam S, Karra M, Benchimol S, Arrowsmith CH
  TITLE     Molecular basis of Pirh2-mediated p53 ubiquitylation.
  JOURNAL   Nat Struct Mol Biol. 2008 Dec . 15(12):1334-42.
  PUBMED    19043414
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol. 2008 Dec . 15(12):1334-42."
COMMENT     Sequence Label: human pirh2-(1-195)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(690..712)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(857..875)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aacggactgg ttccgcgtgg atccccggaa ttcaccatgg cggcgacggc ccgggaagat
       61 ggcgccagcg gtcaagagcg aggtcagcgg ggctgcgagc actatgacag aggatgtctc
      121 ctaaaggcac cttgctgtga caagctttat acttgccgct tgtgtcatga taacaatgaa
      181 gatcatcaac tagatcgctt taaagtgaag gaagtgcagt gcataaactg tgaaaaaatt
      241 caacatgccc aacagacttg tgaagaatgt agcacattgt ttggagaata ttattgcgat
      301 atatgccatt tgtttgacaa agataagaag cagtatcact gtgaaaactg tggaatttgt
      361 aggattggtc caaaggaaga ttttttccat tgtttgaaat gtaacttatg cctagctatg
      421 aatcttcaag gaagacacaa gtgtattgaa aatgtgtccc gacagaattg tccaatatgt
      481 ttggaggaca ttcacacatc ccgtgttgtt gctcatgtct tgccatgtgg acatctttta
      541 catagaacgt gttatgaaga aatgttgaaa gaaggctaca gatgtccatt atgtatgcac
      601 tctgctttag atatgaccag gtgactcgag cggccgcatc gtgactgact gacgatctgc
      661 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
      721 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
      781 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtataat
      841 tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
      901 atggtttctt a
//
