LOCUS       sequence-308244-       13707 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human FLI1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13707)
  AUTHORS   Palii CG, Cheng Q, Gillespie MA, Shannon P, Mazurczyk M, Napolitani 
            G, Price ND, Ranish JA, Morrissey E, Higgs DR, Brand M
  TITLE     Single-Cell Proteomics Reveal that Quantitative Changes in 
            Co-expressed Lineage-Specific Transcription Factors Determine Cell 
            Fate.
  JOURNAL   Cell Stem Cell. 2019 May 2;24(5):812-820.e5. doi: 
            10.1016/j.stem.2019.02.006. Epub 2019 Mar 14.
  PUBMED    30880026
REFERENCE   2  (bases 1 to 13707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2019.02.006"; journalName: "Cell Stem Cell"; date: 
            "2019-05-2- 2"; volume: "24"; issue: "5"; pages: "812-820.e5"
COMMENT     Sequence Label: pTRIPdU3-MND-FLAG-FLI1-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..13707
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2054..2171
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      2767..2776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4129..4152
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4175..4742
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4192..4744
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4336..4353)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4563..4582
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4756..4758,4759..4761,4762..5475)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4801..4822)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5062..5081)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5409..5430
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5779..5959
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7487..7506)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7720..7739)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7839..7861
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7899..7917)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7985..8089
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8090..8158,8159..8950)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9121..9709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11298..11318)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11303..11632
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11483..11618
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12766..12831
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12961..12981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13406..13540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13443..13462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13497..13516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gccgaattca caaatggcag
     2041 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2101 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2161 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggctg atacgcgtca
     2221 tcgatcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     2281 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagatagaa taaaagattt
     2341 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2401 gatcaaggtt aggaacagag agacagcaga atatgggcca aacaggatat ctgtggtaag
     2461 cagttcctgc cccggctcag ggccaagaac agttggaaca gcagaatatg ggccaaacag
     2521 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat
     2581 gcggtcccgc cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga
     2641 cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc
     2701 gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc ggcgcgatct
     2761 ggatctgcca ccatggacgg gactattaag gaggctctgt cggtggtgag cgacgaccag
     2821 tccctctttg actcagcgta cggagcggca gcccatctcc ccaaggccga catgactgcc
     2881 tcggggagtc ctgactacgg gcagccccac aagatcaacc ccctcccacc acagcaggag
     2941 tggatcaatc agccagtgag ggtcaacgtc aagcgggagt atgaccacat gaatggatcc
     3001 agggagtctc cggtggactg cagcgttagc aaatgcagca agctggtggg cggaggcgag
     3061 tccaacccca tgaactacaa cagctatatg gacgagaaga atggcccccc tcctcccaac
     3121 atgaccacca acgagaggag agtcatcgtc cccgcagacc ccacactgtg gacacaggag
     3181 catgtgaggc aatggctgga gtgggccata aaggagtaca gcttgatgga gatcgacaca
     3241 tcctttttcc agaacatgga tggcaaggaa ctgtgtaaaa tgaacaagga ggacttcctc
     3301 cgcgccacca ccctctacaa cacggaagtg ctgttgtcac acctcagtta cctcagggaa
     3361 agttcactgc tggcctataa tacaacctcc cacaccgacc aatcctcacg attgagtgtc
     3421 aaagaagacc cttcttatga ctcagtcaga agaggagctt ggggcaataa catgaattct
     3481 ggcctcaaca aaagtcctcc ccttggaggg gcacaaacga tcagtaagaa tacagagcaa
     3541 cggccccagc cagatccgta tcagatcctg ggcccgacca gcagtcgcct agccaaccct
     3601 ggaagcgggc agatccagct gtggcaattc ctcctggagc tgctctccga cagcgccaac
     3661 gccagctgta tcacctggga ggggaccaac ggggagttca aaatgacgga ccccgatgag
     3721 gtggccaggc gctggggcga gcggaaaagc aagcccaaca tgaattacga caagctgagc
     3781 cgggccctcc gttattacta tgataaaaac attatgacca aagtgcacgg caaaagatat
     3841 gcttacaaat ttgacttcca cggcattgcc caggctctgc agccacatcc gaccgagtcg
     3901 tccatgtaca agtacccttc tgacatctcc tacatgcctt cctaccatgc ccaccagcag
     3961 aaggtgaact ttgtccctcc ccatccatcc tccatgcctg tcacttcctc cagcttcttt
     4021 ggagccgcat cacaatactg gacctccccc acggggggaa tctaccccaa ccccaacgtc
     4081 ccccgccatc ctaacaccca cgtgccttca cacttaggca gctactacga ttacaaggat
     4141 gacgacgata agtgataact cgaggttaac gaattccgcc ccccccccct aacgttactg
     4201 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     4261 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     4321 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     4381 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     4441 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     4501 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     4561 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     4621 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     4681 aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat
     4741 aatatggcca caaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4801 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtctgg cgagggcgag
     4861 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4921 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4981 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     5041 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     5101 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     5161 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5221 gacaagcaga agaacggcat caaggcgaac ttcaagatcc gccacaacat cgaggacggc
     5281 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5341 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     5401 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5461 gagctgtaca agtaatgaat taattaagaa ttatcaagct tatcgatacc gtcgagacct
     5521 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     5581 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     5641 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     5701 ggaagggcta attcactccc aacgaagaca aaatcgtcga gagatgctgc atataagcag
     5761 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     5821 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     5881 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     5941 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt
     6001 gcaaagaaat gaatatcaga gagtgagagg ccttgacatt ataatagatt tagcaggaat
     6061 tgaactagga gtggagcaca caggcaaagc tgcagaagta cttggaagaa gccaccagag
     6121 atactcacga ttctgcacat acctggctaa tcccagatcc taaggattac attaagttta
     6181 ctaacattta tataatgatt tatagtttaa agtataaact tatctaattt actattctga
     6241 cagatattaa ttaatcctca aatatcataa gagatgatta ctattatccc catttaacac
     6301 aagaggaaac tgagagggaa agatgttgaa gtaattttcc cacaattaca gcatccgtta
     6361 gttacgactc tatgatcttc tgacacaaat tccatttact cctcacccta tgactcagtc
     6421 gaatatatca aagttatgga cattatgcta agtaacaaat taccctttta tatagtaaat
     6481 actgagtaga ttgagagaag aaattgtttg caaacctgaa tagcttcaag aagaagagaa
     6541 gtgaggataa gaataacagt tgtcatttaa caagttttaa caagtaactt ggttagaaag
     6601 ggattcaaat gcataaagca agggataaat ttttctggca acaagactat acaatataac
     6661 cttaaatatg acttcaaata attgttggaa cttgataaaa ctaattaaat attattgaag
     6721 attatcaata ttataaatgt aatttacttt taaaaaggga acatagaaat gtgtatcatt
     6781 agagtagaaa acaatcctta ttatcacaat ttgtcaaaac aagtttgtta ttaacacaag
     6841 tagaatactg cattcaatta agttgactgc agattttgtg ttttgttaaa attagaaaga
     6901 gataacaaca atttgaatta ttgaaagtaa catgtaaata gttctacata cgttcttttg
     6961 acatcttgtt caatcattga tcgaagttct ttatcttgga agaatttgtt ccaaagactc
     7021 tgaaataagg aaaacaatct attatatagt ctcacacctt tgttttactt ttagtgattt
     7081 caatttaata atgtaaatgg ttaaaattta ttcttctctg agatcatttc acattgcaga
     7141 tagaaaacct gagactgggg taatttttat taaaatctaa tttaatctca gaaacacatc
     7201 tttattctaa catcaatttt tccagtttga tattatcata taaagtcagc cttcctcatc
     7261 tgcaggttcc acaacaaaaa tccaaccaac tgtggatcaa aaatattggg aaaaaattaa
     7321 aaatagcaat acaacaataa aaaaatacaa atcagaaaaa cagcacagta taacaacttt
     7381 atttagcatt tacaatctat taggtattat aagtaatcta gccagatcct ctacgccgga
     7441 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     7501 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     7561 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     7621 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     7681 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     7741 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7801 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7861 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     7921 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7981 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     8041 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     8101 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     8161 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8221 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8281 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8341 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8401 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8461 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8521 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8581 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8641 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8701 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8761 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8821 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8881 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8941 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     9001 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     9061 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     9121 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9181 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9241 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9301 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9361 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9421 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9481 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9541 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9601 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9661 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9721 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9781 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9841 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9901 acgccgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     9961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    10021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    10081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    10141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    10201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    10261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    10321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    10381 ggcccttccg gcgtccaggc tagcctcttc cagcgcagcc agctgttctt cgttatgcac
    10441 ggcggtatga aaatgttgcg cagaaatcgt cggcagatct ctggcatcaa aaaagccttc
    10501 gagtaacagt acaggtttgg tgattccccc cgcacgcagt cgcagagctt cttcgagacg
    10561 ggctacgcca aaggcgtcag catcggggag cgttcgcgcg gtctcaagaa gaccgtgacc
    10621 ataagcgttc gctttcacca ccgcaaccat tttactggca ggggccagtt cacgaagacg
    10681 ttgcaggttg tgtcgcagag cgcggcggtt aatcammacm kttkccgstt gsmtttktgt
    10741 tccttkrtaa gtgtttkctt twattacctw attcmtwaaa taattattat tygtcgtcgt
    10801 actgcggccc cgcatagttg tcgaagcgcg acaccgammk gagataccta cagcgtgagc
    10861 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10921 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10981 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    11041 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    11101 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    11161 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    11221 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    11281 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    11341 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    11401 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    11461 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    11521 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11581 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
    11641 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
    11701 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    11761 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    11821 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    11881 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    11941 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    12001 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    12061 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    12121 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    12181 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    12241 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    12301 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    12361 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    12421 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    12481 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    12541 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    12601 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    12661 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    12721 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt
    12781 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc
    12841 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc
    12901 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct
    12961 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt
    13021 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag
    13081 gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac ctttataagt
    13141 aggcataaca gttataatca taacatactg ttttttctta ctccacacag gcatagagtg
    13201 tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg
    13261 gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
    13321 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
    13381 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
    13441 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    13501 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    13561 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    13621 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    13681 ttaaatatgt actacaaact tagtagt
//
