LOCUS       sequence-308554-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Sauer M, Juranek SA, Marks J, De Magis A, Kazemier HG, Hilbig D, 
            Benhalevy D, Wang X, Hafner M, Paeschke K
  TITLE     DHX36 prevents the accumulation of translationally inactive mRNAs 
            with G4-structures in untranslated regions.
  JOURNAL   Nat Commun. 2019 Jun 3;10(1):2421. doi: 10.1038/s41467-019-10432-5.
  PUBMED    31160600
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-06-3"; volume: "10(1):2421. doi"; pages: " 
            10.1038/s41467-019-10432-5"
COMMENT     Sequence Label: pFRT-DHX36iso1
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    6..24
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    27..45
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     55..79
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             170..193
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             194..220
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agctctccct atcagtgata gagatctccc tatcagtgat agagatcgtc gacgagctcg
       61 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      121 acaccgggac cgatccagcc tccggactct agcgtttaaa cttaagatgg actacaagga
      181 cgacgatgac aagtacccat acgatgttcc agattacgct ggatccatga gttatgacta
      241 ccatcagaac tggggccgtg atgggggtcc ccgcagctcc ggtgggggct atggaggggg
      301 gccagcaggg ggtcatggag gtaaccgagg ctccggagga ggcggcggcg gcggaggggg
      361 tggtcgaggc ggcaggggcc ggcatcccgg gcacctgaaa ggccgcgaaa tcggcatgtg
      421 gtacgcgaaa aaacaggggc agaagaacaa ggaagcggag aggcaagaga gagctgtagt
      481 acacatggat gaacgacgag aagaacaaat tgtacagtta ctgaattctg ttcaagcgaa
      541 gaatgataaa gagtcagaag cacagatatc ctggtttgct cctgaggatc atggatacgg
      601 tactgaagtt tctactaaga acacaccatg ctcagagaac aaacttgaca tccaggaaaa
      661 gaagttgata aatcaagaaa aaaaaatgtt tagaatcagg aacagatcat atattgaccg
      721 agattctgag tatctcttgc aagaaaatga accagatgga actttagacc aaaaattatt
      781 ggaagattta caaaagaaaa aaatgacctt cggtatattg aaatgcagca tttcagagaa
      841 aagctgcctt cgtatggaat gcaaaaggaa ttggtaaatt taattgataa ccatcaggta
      901 acagtaataa gtggtgaaac tggttgtggc aaaccactca agttactcag ttcttttgga
      961 tactacattg aaagaggaaa ggatctgctt gcaaatagtt gtactca
//
