LOCUS       sequence-308717-        6071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 
            154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of 
            PHYB was mutated to Histidine. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Buckley CE, Moore RE, Reade A, Goldberg AR, Weiner OD, Clarke JD
  TITLE     Reversible Optogenetic Control of Subcellular Protein Localization 
            in a Live Vertebrate Embryo.
  JOURNAL   Dev Cell. 2016 Jan 11;36(1):117-26. doi: 
            10.1016/j.devcel.2015.12.011.
  PUBMED    26766447
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.12"; journalName: "Dev Cell"; date: 
            "2016-01-11- 11"; volume: "36"; issue: "1"; pages: "117-26"
COMMENT     Sequence Label: pCS2+-Y276H PHYB-CAAX
FEATURES             Location/Qualifiers
     source          1..6071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      109..118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2123..2257
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2147..2166)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2201..2220
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2370..2390)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2370..2388)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2409..2425)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2409..2425)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2422..2444)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2433..2449
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2457..2463,2464..2481,2482..2487))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2502..2523
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2640..2657)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2811..3399)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2891..2910)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3570..4361,4362..4430))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4193..4212
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4431..4535)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4561..5016)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4698..4719)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4910..4929
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5087..6071
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattca aggcgcgtgc caccatggta
      121 tcaggtgttg gaggaagtgg tggaggtaga ggtggaggaa gaggtggaga agaagagccc
      181 tcaagttcac acactcccaa caatagacga gggggtgaac aagcgcagtc ttctggtacg
      241 aagtccctcc ggcctcgctc taatacagaa tccatgtcca aagccatcca gcagtataca
      301 gtggatgcca ggctccacgc agtttttgaa cagagcgggg aaagtgggaa atctttcgat
      361 tatagccaga gtctgaagac cacaacttac ggcagcagtg ttccagagca acagattacg
      421 gcttacctta gccggataca gcgcggaggc tacatacagc cttttgggtg tatgatcgcg
      481 gttgacgaat ctagcttccg cataatcgga tattctgaaa acgctcggga gatgctgggt
      541 atcatgccac agagcgttcc tacactggaa aagcctgaaa tcctcgcgat gggcaccgat
      601 gtgcgcagcc tcttcacgtc atcttcaagt atcctcctgg agcgcgcttt tgttgcccgg
      661 gaaatcacac ttctgaatcc tgtctggatt catagcaaga atacagggaa acccttttac
      721 gctatcctgc acagaatcga tgtcggggtt gtaatagacc ttgagccagc tagaacagaa
      781 gatccggcct tgagcatcgc tggcgcagtg caaagccaga agctggcagt gagggcaata
      841 tctcaactgc aagccctgcc aggcggcgat ataaagctgc tgtgtgatac cgtagtggag
      901 agcgttcgcg atctgaccgg ttatgacaga gtgatggtcc acaaatttca tgaagatgag
      961 catggggagg tagtggcaga atcaaaaaga gacgacttgg aaccctatat cggactccac
     1021 tatcctgcta ctgatattcc ccaggcaagt cggtttttgt ttaagcagaa tagggtcagg
     1081 atgattgtgg actgtaatgc tactcccgtg ttggtagtcc aagacgatcg actgacccaa
     1141 tctatgtgcc tggtggggag tacactgcgc gcaccacacg ggtgccattc ccagtatatg
     1201 gcgaacatgg ggagcatcgc ctcactggca atggctgtaa tcatcaacgg taacgaggat
     1261 gatggatcta atgtcgccag tggcagatcc tcaatgcgcc tctgggggct ggtcgtctgc
     1321 catcatacaa gcagccggtg tattcccttt ccgctgaggt acgcttgtga gtttcttatg
     1381 caagcatttg ggcttcagct taatatggaa ctccagcttg cccttcaaat gagtgagaag
     1441 cgggttctcc gcactcagac ccttctctgc gatatgcttt tgcgcgattc ccccgctggc
     1501 atcgtgaccc agagcccttc aataatggac cttgtaaagt gcgatggtgc tgcattcctg
     1561 tatcatggga aatactatcc actgggagtg gccccaagcg aagtacagat aaaggacgtg
     1621 gtcgagtggc tgctcgccaa tcacgccgac agtacagggc tgagcaccga tagtctgggg
     1681 gacgccggat atccaggtgc cgccgcactt ggcgatgcag tgtgtggcat ggcagtcgcc
     1741 tatattacga agcgcgattt tctcttttgg tttcgatcac acacagcaaa agagataaag
     1801 tggggaggcg ccaagcacca tccagaggat aaggatgacg ggcaaagaat gcatccaaga
     1861 agttctttcc aagccttcct cgaagtggtt aagtcacgat cacagccttg ggaaaccgcc
     1921 gaaatggacg ctatccattc tctccagctg atcctgcggg acagtttcaa ggagagcggt
     1981 agcggttcag gttcaggtag cggatccctc gagaaaatgt ccaaggatgg taagaaaaag
     2041 aagaagaagt caaaaaccaa gtgtgttatc atgtaagcgg cccctctcga gcctctagaa
     2101 ctatagtgag tcgtattacg tagatccaga catgataaga tacattgatg agtttggaca
     2161 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2221 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2281 tatgtttcag gttcaggggg aggtgtggga ggttttttaa ttcgcggccg cggcgccaat
     2341 gcattgggcc cggtacccag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg
     2401 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2461 atacgagccg ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2521 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     2581 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     2641 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     2701 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     2761 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2821 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2881 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2941 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     3001 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     3061 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     3121 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3181 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3241 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     3301 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3361 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3421 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3481 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3541 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3601 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3661 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3721 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3781 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3841 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     3901 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     3961 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4021 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4081 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4141 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4201 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4261 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4321 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4381 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     4441 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     4501 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     4561 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
     4621 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
     4681 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
     4741 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
     4801 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
     4861 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
     4921 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
     4981 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
     5041 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagtcg accgccaatt
     5101 caatatggcg tatatggact catgccaatt caatatggtg gatctggacc tgtgccaatt
     5161 caatatggcg tatatggact cgtgccaatt caatatggtg gatctggacc ccagccaatt
     5221 caatatggcg gacttggcac catgccaatt caatatggcg gacctggcac tgtgccaact
     5281 ggggaggggt ctacttggca cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag
     5341 tccgccatat tgaattggca tggtgccaat aatggcggcc atattggcta tatgccagga
     5401 tcaatatata ggcaatatcc aatatggccc tatgccaata tggctattgg ccaggttcaa
     5461 tactatgtat tggccctatg ccatatagta ttccatatat gggttttcct attgacgtag
     5521 atagcccctc ccaatgggcg gtcccatata ccatatatgg ggcttcctaa taccgcccat
     5581 agccactccc ccattgacgt caatggtctc tatatatggt ctttcctatt gacgtcatat
     5641 gggcggtcct attgacgtat atggcgcctc ccccattgac gtcaattacg gtaaatggcc
     5701 cgcctggctc aatgcccatt gacgtcaata ggaccaccca ccattgacgt caatgggatg
     5761 gctcattgcc cattcatatc cgttctcacg ccccctattg acgtcaatga cggtaaatgg
     5821 cccacttggc agtacatcaa tatctattaa tagtaacttg gcaagtacat tactattgga
     5881 agtacgccag ggtacattgg cagtactccc attgacgtca atggcggtaa atggcccgcg
     5941 atggctgcca agtacatccc cattgacgtc aatggggagg ggcaatgacg caaatgggcg
     6001 ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc
     6061 gtttagggaa c
//
