LOCUS       sequence-308793-       11570 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral packaging vector for CEACAM6 expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11570)
  AUTHORS   Sheikh A, Tumala B, Vickers TJ, Alvarado D, Ciorba MA, Bhuiyan TR, 
            Qadri F, Singer BB, Fleckenstein JM
  TITLE     CEACAMs serve as toxin-stimulated receptors for enterotoxigenic 
            Escherichia coli.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Nov 17;117(46):29055-29062. doi: 
            10.1073/pnas.2012480117. Epub 2020 Nov 2.
  PUBMED    33139570
REFERENCE   2  (bases 1 to 11570)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.2012480117"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2020-11-17- 17"; volume: "117"; issue: "46"; pages: 
            "29055-29062"
COMMENT     Sequence Label: pFCIV-CEACAM6
FEATURES             Location/Qualifiers
     source          1..11570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     misc_feature    join(2294..2868,2869..2871,2872..2880)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2461..2478)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2688..2707
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(2881..2883,2884..2886,2887..3600)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2926..2947)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3187..3206)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3534..3555
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3641..4229
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3694..3714)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4112..4123)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4232..4248)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4233..4249)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4754..4934
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4960..4977)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4966..5190
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5236..5664
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5323..5342)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5533..5554
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5673..5693)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5678..6007
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5858..5993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5920..5939
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6055..6102
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6121..6495
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6625..6746
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6662..6681)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6716..6735
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6795..6811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6795..6811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6808..6830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6819..6835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6843..6849,6850..6867,6868..6873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6888..6909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7026..7043)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7197..7785)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7277..7296)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7956..8747,8748..8816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8579..8598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8817..8921)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8996..9015)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9187..9566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9568..9766
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9721..9741
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             9784..9964
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10011..10136
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10629..10862
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11047..11091
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11389..11506
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatccatgg gacccccctc
     1261 agcccctccc tgcagattgc atgtcccctg gaaggaggtc ctgctcacag cctcacttct
     1321 aaccttctgg aacccaccca ccactgccaa gctcactatt gaatccacgc cattcaatgt
     1381 cgcagagggg aaggaggttc ttctactcgc ccacaacctg ccccagaatc gtattggtta
     1441 cagctggtac aaaggcgaaa gagtggatgg caacagtcta attgtaggat atgtaatagg
     1501 aactcaacaa gctaccccag ggcccgcata cagtggtcga gagacaatat accccaatgc
     1561 atccctgctg atccagaacg tcacccagaa tgacacagga ttctataccc tacaagtcat
     1621 aaagtcagat cttgtgaatg aagaagcaac cggacagttc catgtatacc cggagctgcc
     1681 caagccctcc atctccagca acaactccaa ccccgtggag gacaaggatg ctgtggcctt
     1741 cacctgtgaa cctgaggttc agaacacaac ctacctgtgg tgggtaaatg gtcagagcct
     1801 cccggtcagt cccaggctgc agctgtccaa tggcaacatg accctcactc tactcagcgt
     1861 caaaaggaac gatgcaggat cctatgaatg tgaaatacag aacccagcga gtgccaaccg
     1921 cagtgaccca gtcaccctga atgtcctcta tggcccagat gtccccacca tttccccctc
     1981 aaaggccaat taccgtccag gggaaaatct gaacctctcc tgccacgcag cctctaaccc
     2041 acctgcacag tactcttggt ttatcaatgg gacgttccag caatccacac aagagctctt
     2101 tatccccaac atcactgtga ataatagcgg atcctatatg tgccaagccc ataactcagc
     2161 cactggcctc aataggacca cagtcacgat gatcacagtc tctggaagtg ctcctgtcct
     2221 ctcagctgtg gccaccgtcg gcatcacgat tggagtgctg gccagggtgg ctctgatata
     2281 aaccggtgat ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     2341 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     2401 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     2461 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     2521 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     2581 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     2641 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     2701 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     2761 tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     2821 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc
     2881 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     2941 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3001 ggcaagctga ccctgaagct gatctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3061 ctcgtgacca ccctgggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
     3121 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3181 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3241 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3301 aagctggagt acaactacaa cagccacaac gtctatatca ccgccgacaa gcagaagaac
     3361 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcggcgt gcagctcgcc
     3421 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3481 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3541 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     3601 agcggccgcg actctagaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
     3661 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3721 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     3781 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     3841 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     3901 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     3961 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4021 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4081 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4141 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4201 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
     4261 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
     4321 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
     4381 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     4441 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
     4501 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
     4561 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
     4621 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     4681 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
     4741 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     4801 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     4861 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     4921 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
     4981 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5041 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5101 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
     5161 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
     5221 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     5281 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     5341 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     5401 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     5461 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     5521 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     5581 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     5641 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
     5701 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5761 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     5821 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     5881 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     5941 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6001 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
     6061 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
     6121 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
     6181 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
     6241 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
     6301 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
     6361 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
     6421 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
     6481 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6541 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6601 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6661 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6721 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6781 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6841 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6901 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6961 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7021 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7081 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7141 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7201 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7261 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7321 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7381 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7441 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7501 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7561 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7621 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7681 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7741 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7801 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8821 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8881 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8941 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     9001 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     9061 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     9121 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     9181 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
     9241 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
     9301 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
     9361 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
     9421 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
     9481 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
     9541 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
     9601 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
     9661 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
     9721 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
     9781 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9841 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9901 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     9961 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
    10021 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
    10081 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
    10141 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
    10201 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
    10261 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
    10321 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
    10381 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
    10441 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
    10501 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
    10561 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
    10621 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
    10681 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
    10741 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
    10801 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
    10861 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
    10921 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
    10981 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
    11041 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
    11101 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
    11161 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
    11221 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
    11281 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
    11341 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
    11401 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    11461 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    11521 ggacagcaga gatccagttt ggttaattaa cccgtgtcgg ctccagatct
//
