LOCUS       sequence-308799-       13279 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heat shock promoter driven expression of human APOB48-mCherry. The 
            construct has tol2 elements, and is designed to use in zebrafish..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13279)
  AUTHORS   Walsh MT, Celestin OM, Thierer JH, Rajan S, Farber SA, Hussain MM
  TITLE     Model systems to study assembly, trafficking and secretion of 
            apoB-lipoproteins using fluorescent fusion proteins.
  JOURNAL   J Lipid Res. 2019 Dec 30. pii: jlr.RA119000259. doi: 
            10.1194/jlr.RA119000259.
  PUBMED    31888978
REFERENCE   2  (bases 1 to 13279)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Lipid 
            Res. 2019 Dec 30. pii: jlr.RA119000259. doi: 
            10.1194/jlr.RA119000259."
COMMENT     Sequence Label: hsp70l: hAPOB48-mCherry.
FEATURES             Location/Qualifiers
     source          1..13279
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1541..1565
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(8109..8133)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             8135..8845
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEDDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8280..8298)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     8548..8567
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8742..8765
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    8883..9017
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9080..9100)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(9108..9124)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    9140..9274
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    join(9713..9900,9901..9912)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(9946..9962)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9946..9962)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9999..10019)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9999..10017)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10038..10054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10051..10073)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10062..10078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10086..10092,10093..10110,10111..10116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10131..10152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10269..10286)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10440..11028)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10520..10539)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11199..11990,11991..12059))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11822..11841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12060..12164)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12327..12348)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(12697..12708,12709..12871)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     13244..13260
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caactttgta tagaaaagtt gcttcagggg tgtcgcttgg ttatttccaa aaatcaaatt
       61 aattttatta aactattaga acgagcatgt tttgtctata tgctacagaa gataaaaaat
      121 aataggagtt aacagttata aaacaacaca ctttgtttct attgattgtt gaccacactg
      181 gggtctcatt aagttagatt aaagacacac taactgggtc aaaagcagca gattgatttc
      241 atagcaccag ggtaaacttt ctaacacttt tacggcaatc atatacatta aaattaaata
      301 cagaccacga ctgaacaagg aggatgatct ccaatattaa acaaagagac ttgtgcctat
      361 ttctctgagg gtaaacatga cctctcaagt tagcaagttg gttttaacac tacaaaaata
      421 gttaagactg caatcccaga ataaagtatt ggttttaacc aatcaatata gtacagtaaa
      481 catccatttg ttttgttgaa acgttaaaca aatctgacca aagctattag cttatataaa
      541 acaggtttgc cttctatgta gctgaaaaca ccacaggccc gattttgcta ctgtgtaaaa
      601 catttcagca agattttttt attgcatttt tttttactga atcgttcaaa cattttatca
      661 ttttagtttg ttcattcatt gcaactggaa aaacaacaca tcacacaacc gcacatattt
      721 cagcaataag tacaataaaa cactcaaata aaaaaaacat tttaaatctc tttgtatttt
      781 tgaccgctgt ttcgcgtaat ttcacggtaa aactctggaa atctccacta cattcctctc
      841 agcggctcct ctcaatgaca gctgaagaag tgacgcggct gcctgctgtg ttttgattgg
      901 tcgaattcac tggaggcttc cagaacagtg tagagtctga acgggtgcgc gctctgctgt
      961 atttaaaggg cgaaagagag accgcagaga aactcaaccg aagagaagcg acttgacaaa
     1021 gaagaaaaga gcagcctgac aggacttttt ccccgacgag gtgtttattc gctctattta
     1081 agaatctact gtaaggtaag tctcaatata ttgtactcta ttggctaatc agaattatat
     1141 agagattata tgtacttaat gtcaaaaaat caactttgta tatgtaatct ttttacatgt
     1201 ggactgccta tgttcatctt attttaggtc tactagaaaa ttatatttcc cgttttcaca
     1261 ataaggattt ttaaaaaaag caatgaacag acgggcattt actttatgtt gctgacatta
     1321 ttttatatga gcataataac cataaatact agcaaatgtc ctaaatgaat ttgtgttaat
     1381 gttgtctaca aaagaaaatt agcgttttac ttgtacaact aataataact tggttattaa
     1441 gagaatttca cttgttgact agaaaaatcc tttcataatg aaacaattgc accataaatt
     1501 gtataaatat aaaattaatt ctaattgttt ttttttcctg ccaagtttgt acaaaaaagc
     1561 aggctcaatg gacccgccga ggcccgcgct gctggcgctg ctggcgctgc ctgcgctgct
     1621 gctgctgctg ctggcgggcg ccagggccga agaggaaatg ctggaaaatg tcagcctggt
     1681 ctgtccaaaa gatgcgaccc gattcaagca cctccggaag tacacataca actatgaggc
     1741 tgagagttcc agtggagtcc ctgggactgc tgattcaaga agtgccacca ggatcaactg
     1801 caaggttgag ctggaggttc cccagctctg cagcttcatc ctgaagacca gccagtgcac
     1861 cctgaaagag gtgtatggct tcaaccctga gggcaaagcc ttgctgaaga aaaccaagaa
     1921 ctctgaggag tttgctgcag ccatgtccag gtatgagctc aagctggcca ttccagaagg
     1981 gaagcaggtt ttcctttacc cggagaaaga tgaacctact tacatcctga acatcaagag
     2041 gggcatcatt tctgccctcc tggttccccc agagacagaa gaagccaagc aagtgttgtt
     2101 tctggatacc gtgtatggaa actgctccac tcactttacc gtcaagacga ggaagggcaa
     2161 tgtggcaaca gaaatatcca ctgaaagaga cctggggcag tgtgatcgct tcaagcccat
     2221 ccgcacaggc atcagcccac ttgctctcat caaaggcatg acccgcccct tgtcaactct
     2281 gatcagcagc agccagtcct gtcagtacac actggacgct aagaggaagc atgtggcaga
     2341 agccatctgc aaggagcaac acctcttcct gcctttctcc tacaagaata agtatgggat
     2401 ggtagcacaa gtgacacaga ctttgaaact tgaagacaca ccaaagatca acagccgctt
     2461 ctttggtgaa ggtactaaga agatgggcct cgcatttgag agcaccaaat ccacatcacc
     2521 tccaaagcag gccgaagctg ttttgaagac tctccaggaa ctgaaaaaac taaccatctc
     2581 tgagcaaaat atccagagag ctaatctctt caataagctg gttactgagc tgagaggcct
     2641 cagtgatgaa gcagtcacat ctctcttgcc acagctgatt gaggtgtcca gccccatcac
     2701 tttacaagcc ttggttcagt gtggacagcc tcagtgctcc actcacatcc tccagtggct
     2761 gaaacgtgtg catgccaacc cccttctgat agatgtggtc acctacctgg tggccctgat
     2821 ccccgagccc tcagcacagc agctgcgaga gatcttcaac atggcgaggg atcagcgcag
     2881 ccgagccacc ttgtatgcgc tgagccacgc ggtcaacaac tatcataaga caaaccctac
     2941 agggacccag gagctgctgg acattgctaa ttacctgatg gaacagattc aagatgactg
     3001 cactggggat gaagattaca cctatttgat tctgcgggtc attggaaata tgggccaaac
     3061 catggagcag ttaactccag aactcaagtc ttcaatcctg aaatgtgtcc aaagtacaaa
     3121 gccatcactg atgatccaga aagctgccat ccaggctctg cggaaaatgg agcctaaaga
     3181 caaggaccag gaggttcttc ttcagacttt ccttgatgat gcttctccgg gagataagcg
     3241 actggctgcc tatcttatgt tgatgaggag tccttcacag gcagatatta acaaaattgt
     3301 ccaaattcta ccatgggaac agaatgagca agtgaagaac tttgtggctt cccatattgc
     3361 caatatcttg aactcagaag aattggatat ccaagatctg aaaaagttag tgaaagaagc
     3421 tctgaaagaa tctcaacttc caactgtcat ggacttcaga aaattctctc ggaactatca
     3481 actctacaaa tctgtttctc ttccatcact tgacccagcc tcagccaaaa tagaagggaa
     3541 tcttatattt gatccaaata actaccttcc taaagaaagc atgctgaaaa ctaccctcac
     3601 tgcctttgga tttgcttcag ctgacctcat cgagattggc ttggaaggaa aaggctttga
     3661 gccaacattg gaagctcttt ttgggaagca aggatttttc ccagacagtg tcaacaaagc
     3721 tttgtactgg gttaatggtc aagttcctga tggtgtctct aaggtcttag tggaccactt
     3781 tggctatacc aaagatgata aacatgagca ggatatggta aatggaataa tgctcagtgt
     3841 tgagaagctg attaaagatt tgaaatccaa agaagtcccg gaagccagag cctacctccg
     3901 catcttggga gaggagcttg gttttgccag tctccatgac ctccagctcc tgggaaagct
     3961 gcttctgatg ggtgcccgca ctctgcaggg gatcccccag atgattggag aggtcatcag
     4021 gaagggctca aagaatgact tttttcttca ctacatcttc atggagaatg cctttgaact
     4081 ccccactgga gctggattac agttgcaaat atcttcatct ggagtcattg ctcccggagc
     4141 caaggctgga gtaaaactgg aagtagccaa catgcaggct gaactggtgg caaaaccctc
     4201 cgtgtctgtg gagtttgtga caaatatggg catcatcatt ccggacttcg ctaggagtgg
     4261 ggtccagatg aacaccaact tcttccacga gtcgggtctg gaggctcatg ttgccctaaa
     4321 agctgggaag ctgaagttta tcattccttc cccaaagaga ccagtcaagc tgctcagtgg
     4381 aggcaacaca ttacatttgg tctctaccac caaaacggag gtgatcccac ctctcattga
     4441 gaacaggcag tcctggtcag tttgcaagca agtctttcct ggcctgaatt actgcacctc
     4501 aggcgcttac tccaacgcca gctccacaga ctccgcctcc tactatccgc tgaccgggga
     4561 caccagatta gagctggaac tgaggcctac aggagagatt gagcagtatt ctgtcagcgc
     4621 aacctatgag ctccagagag aggacagagc cttggtggat accctgaagt ttgtaactca
     4681 agcagaaggt gcgaagcaga ctgaggctac catgacattc aaatataatc ggcagagtat
     4741 gaccttgtcc agtgaagtcc aaattccgga ttttgatgtt gacctcggaa caatcctcag
     4801 agttaatgat gaatctactg agggcaaaac gtcttacaga ctcaccctgg acattcagaa
     4861 caagaaaatt actgaggtcg ccctcatggg ccacctaagt tgtgacacaa aggaagaaag
     4921 aaaaatcaag ggtgttattt ccataccccg tttgcaagca gaagccagaa gtgagatcct
     4981 cgcccactgg tcgcctgcca aactgcttct ccaaatggac tcatctgcta cagcttatgg
     5041 ctccacagtt tccaagaggg tggcatggca ttatgatgaa gagaagattg aatttgaatg
     5101 gaacacaggc accaatgtag ataccaaaaa aatgacttcc aatttccctg tggatctctc
     5161 cgattatcct aagagcttgc atatgtatgc taatagactc ctggatcaca gagtccctca
     5221 aacagacatg actttccggc acgtgggttc caaattaata gttgcaatga gctcatggct
     5281 tcagaaggca tctgggagtc ttccttatac ccagactttg caagaccacc tcaatagcct
     5341 gaaggagttc aacctccaga acatgggatt gccagacttc cacatcccag aaaacctctt
     5401 cttaaaaagc gatggccggg tcaaatatac cttgaacaag aacagtttga aaattgagat
     5461 tcctttgcct tttggtggca aatcctccag agatctaaag atgttagaga ctgttaggac
     5521 accagccctc cacttcaagt ctgtgggatt ccatctgcca tctcgagagt tccaagtccc
     5581 tacttttacc attcccaagt tgtatcaact gcaagtgcct ctcctgggtg ttctagacct
     5641 ctccacgaat gtctacagca acttgtacaa ctggtccgcc tcctacagtg gtggcaacac
     5701 cagcacagac catttcagcc ttcgggctcg ttaccacatg aaggctgact ctgtggttga
     5761 cctgctttcc tacaatgtgc aaggatctgg agaaacaaca tatgaccaca agaatacgtt
     5821 cacactatca tgtgatgggt ctctacgcca caaatttcta gattcgaata tcaaattcag
     5881 tcatgtagaa aaacttggaa acaacccagt ctcaaaaggt ttactaatat tcgatgcatc
     5941 tagttcctgg ggaccacaga tgtctgcttc agttcatttg gactccaaaa agaaacagca
     6001 tttgtttgtc aaagaagtca agattgatgg gcagttcaga gtctcttcgt tctatgctaa
     6061 aggcacatat ggcctgtctt gtcagaggga tcctaacact ggccggctca atggagagtc
     6121 caacctgagg tttaactcct cctacctcca aggcaccaac cagataacag gaagatatga
     6181 agatggaacc ctctccctca cctccacctc tgatctgcaa agtggcatca ttaaaaatac
     6241 tgcttcccta aagtatgaga actacgagct gactttaaaa tctgacacca atgggaagta
     6301 taagaacttt gccacttcta acaagatgga tatgaccttc tctaagcaaa atgcactgct
     6361 gcgttctgaa tatcaggctg attacgagtc attgaggttc ttcagcctgc tttctggatc
     6421 actaaattcc catggtcttg agttaaatgc tgacatctta ggcactgaca aaattaatag
     6481 tggtgctcac aaggcgacac taaggattgg ccaagatgga atatctacca gtgcaacgac
     6541 caacttgaag tgtagtctcc tggtgctgga gaatgagctg aatgcagagc ttggcctctc
     6601 tggggcatct atgaaattaa caacaaatgg ccgcttcagg gaacacaatg caaaattcag
     6661 tctggatggg aaagccgccc tcacagagct atcactggga agtgcttatc aggccatgat
     6721 tctgggtgtc gacagcaaaa acattttcaa cttcaaggtc agtcaagaag gacttaagct
     6781 ctcaaatgac atgatgggct catatgctga aatgaaattt gaccacacaa acagtctgaa
     6841 cattgcaggc ttatcactgg acttctcttc aaaacttgac aacatttaca gctctgacaa
     6901 gttttataag caaactgtta atttacagct acagccctat tctctggtaa ctactttaaa
     6961 cagtgacctg aaatacaatg ctctggatct caccaacaat gggaaactac ggctagaacc
     7021 cctgaagctg catgtggctg gtaacctaaa aggagcctac caaaataatg aaataaaaca
     7081 catctatgcc atctcttctg ctgccttatc agcaagctat aaagcagaca ctgttgctaa
     7141 ggttcagggt gtggagttta gccatcggct caacacagac atcgctgggc tggcttcagc
     7201 cattgacatg agcacaaact ataattcaga ctcactgcat ttcagcaatg tcttccgttc
     7261 tgtaatggcc ccgtttacca tgaccatcga tgcacataca aatggcaatg ggaaactcgc
     7321 tctctgggga gaacatactg ggcagctgta tagcaaattc ctgttgaaag cagaacctct
     7381 ggcatttact ttctctcatg attacaaagg ctccacaagt catcatctcg tgtctaggaa
     7441 aagcatcagt gcagctcttg aacacaaagt cagtgccctg cttactccag ctgagcagac
     7501 aggcacctgg aaactcaaga cccaatttaa caacaatgaa tacagccagg acttggatgc
     7561 ttacaacact aaagataaaa ttggcgtgga gcttactgga cgaactctgg ctgacctaac
     7621 tctactagac tccccaatta aagtgccact tttactcagt gagcccatca atatcattga
     7681 tgctttagag atgagagatg ccgttgagaa gccccaagaa tttacaattg ttgcttttgt
     7741 aaagtatgat aaaaaccaag atgttcactc cattaacctc ccattttttg agaccttgca
     7801 agaatatttt gagaggaatc gacaaaccat tatagttgta ctggaaaacg tacagagaaa
     7861 cctgaagcac atcaatattg atcaatttgt aagaaaatac agagcagccc tgggaaaact
     7921 cccacagcaa gctaatgatt atctgaattc attcaattgg gagagacaag tttcacatgc
     7981 caaggagaaa ctgactgctc tcacaaaaaa gtatagaatt acagaaaatg atatacaaat
     8041 tgcattagat gatgccaaaa tcaactttaa tgaaaaacta tctcaactgc agacatatat
     8101 gatacaagac ccagctttct tgtacaaagt ggttatggtg agcaagggcg aggacgacaa
     8161 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     8221 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     8281 caagctgaag gtgaccaagg gcggccccct gcccttcgcc tgggacatcc tgtcccctca
     8341 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     8401 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     8461 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     8521 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     8581 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     8641 gctgaagctg aaggacggcg gccactacga cgccgaggtc aagaccacct acaaggccaa
     8701 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagctggaca tcacctccca
     8761 caacgaggac tacaccatcg tggaacagta cgagcgcgcc gagggccgcc actccaccgg
     8821 cggcatggac gagctgtaca agtaaggcgc gcctctagaa ctatagtgag tcgtattacg
     8881 tagatccaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt
     8941 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa
     9001 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg
     9061 aggtgtggga ggttttttcc aactttatta tacatagttg ataattcact ggccgtcgtt
     9121 ttacggtacc atcgatgatg atccagacat gataagatac attgatgagt ttggacaaac
     9181 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
     9241 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     9301 gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg
     9361 tggtatggct gattatgatc ctctagatca gatctgcgaa gatacggcca cgggtgctct
     9421 tgatcctgtg gctgattttg gactgtgctg ctcgcagctg ctgatgaatc acatacttcc
     9481 tccattttct tccactgatt gactgttata atttccctaa tttccaggtc aaggtgctgt
     9541 gcattgtggt aatagatgtg acatgacgtc acttccaaag gaccaatgaa catgtctgac
     9601 caatttcata taatgtgaaa acgattttca taggcagaat aaataacatt taaattaaac
     9661 tgggcatcag cgcaattcaa ttggtttggt aatagcaagg gaaaatagaa tgaagtgatc
     9721 tccaaaaaat aagtactttt tgactgtaaa taaaattgta aggagtaaaa agtacttttt
     9781 tttctaaaaa aatgtaatta agtaaaagta aaagtattga tttttaattg tactcaagta
     9841 aagtaaaaat ccccaaaaat aatacttaag tacagtaatc aagtaaaatt actcaagtac
     9901 tttacacctc tggttcttga ccccctacct tcagcaagcc cagcagatcc actagttcta
     9961 gagcggccgc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattgcg
    10021 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
    10081 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
    10141 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
    10201 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
    10261 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
    10321 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
    10381 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
    10441 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
    10501 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
    10561 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
    10621 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
    10681 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
    10741 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
    10801 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
    10861 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
    10921 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
    10981 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
    11041 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg cattttggtc
    11101 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
    11161 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aataagtgag
    11221 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
    11281 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
    11341 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
    11401 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
    11461 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
    11521 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
    11581 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
    11641 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
    11701 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
    11761 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
    11821 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
    11881 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
    11941 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    12001 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    12061 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    12121 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    12181 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
    12241 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
    12301 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
    12361 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca cgctggctaa
    12421 gaactcatca gcctccccgg tccatctacc cacgtaccaa tgcaccaatt ggccacaatg
    12481 acggctacta catggtgcca ttccttcctc tttataggaa tggagactac ctcctgtcca
    12541 acaatgctct tggatacgag tacgcctacc tgttggaccc aggtcattgc acaacaccag
    12601 aaatgccctc tgatctgcaa aagacgtgaa tatctgttca gacacccata tccactctgt
    12661 tccacacagg tcagaggttt gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa
    12721 attttactca agtgaaagta caagtactta gggaaaattt tactcaatta aaagtaaaag
    12781 tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa attgtacttg
    12841 agtattaagg aagtaaaagt aaaagcaaga aagaaaacta gagattcttg tttaagcttt
    12901 taatctcaaa aaacattaaa tgaaatgcat acaaggtttt atcctgcttt agaactgttt
    12961 gtatttaatt atcaaactat aagacagaca atctaatgcc agtacacgct actcaaagtt
    13021 gtaaaacctc agatttaact tcagtagaag ctgattctca aaattgttag tgtcaagcct
    13081 agctcttttg gggctgaaaa gcaatcctgc agtgctgaaa agcctctcac aggcagccga
    13141 tgcgggaaga ggtgtattag tcttgataga gaggctgcaa atagcaggaa acgtgagcag
    13201 agactccctg gtgtctgaaa cacaggccag atgggccctc gagcaggaaa cagctatgac
    13261 catgattacg ccaagctat
//
