LOCUS       sequence-30906-e         881 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Karanicolas J, Corn JE, Chen I, Joachimiak LA, Dym O, Peck SH, 
            Albeck S, Unger T, Hu W, Liu G, Delbecq S, Montelione GT, Spiegel 
            CP, Liu DR, Baker D
  TITLE     A de novo protein binding pair by computational design and directed 
            evolution.
  JOURNAL   Mol Cell. 2011 Apr 22;42(2):250-60. Epub 2011 Mar 31.
  PUBMED    21458342
REFERENCE   2  (bases 1 to 881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2011-04-22"; volume: "42(2)"; pages: "250-60. Epub 2011 Mar 
            31"
COMMENT     Sequence Label: PrbC5
FEATURES             Location/Qualifiers
     source          1..881
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(545..565)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(566..583)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             603..625
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(713..732)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(714..732)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(816..835)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttctttgggc ttgttagcag ccggatcagc gcgcacgcgt ggtaccggcc ggccaccagg
       61 tggcgcgccg cgatcgcgga tccgttatcc acttccaatg ttattatttt tcgcccagca
      121 ggcgtttgtg ctcacgcatc atacagcgat tagcaacgat aatcaggcct gctcccttgg
      181 cttgacgcgc agccagcgga taatatgtgt tgtactgaaa ccataccact tttgcccctt
      241 tcttgatggc ataggcaacg aagcgccacg ctttagccgg gttcacaaac agatctacga
      301 cctcaatttt atccgggatg tccagaacac tcgggtagca cttgcggccc agcacttctt
      361 cataattcgg gtttaccggg tagacatcat agccatgctc cagcagatat ttcataacaa
      421 tatttgcgtc gcgttccggt ttcggagagg cccccaccag agcgatcttt ttataacgag
      481 tcagaatctc gcggatacct tcgtccgtca gaccatcaat cggacgggtg gatcccattg
      541 cattggattg gaagtacagg ttttcatggt gatggtgatg gtgagaagat ttcatatgta
      601 tatctccttc ttaaagttaa acaaaattat ttctagaact cgagtcctgc agggcggccg
      661 cttaattaaa gagctctccg gagccggcac tagagggaaa ccgttgtggt ctccctatag
      721 tgagtcgtat taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc
      781 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
      841 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt t
//
