LOCUS       sequence-30916-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Albeck JG, Burke JM, Spencer SL, Lauffenburger DA, Sorger PK
  TITLE     Modeling a snap-action, variable-delay switch controlling extrinsic 
            cell death.
  JOURNAL   PLoS Biol. 2008 Dec 2. 6(12):2831-52.
  PUBMED    19053173
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Biol. 
            2008 Dec 2. 6(12):2831-52."
COMMENT     Sequence Label: pBabe-puro-IMS-RP
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             188..865
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             188..865
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(318..336)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     586..605
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     780..803
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ggcggctctg aagagttggc tgtcgcgcag cgtaacttca ttcttcaggt acagacagtg
       61 tttgtgtgtt cctgttgtgg ctaactttaa gaagcggtgt ttctcagaat tgataagacc
      121 atggcacaaa actgtgacga ttggctttgg agtaaccctg tgtggatcag gaggatcagg
      181 agatccgatg gcctcctccg aggacgtcat caaggagttc atgcgcttca aggtgcgcat
      241 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      301 cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc ccttcgcctg
      361 ggacatcctg tcccctcagt tccagtacgg ctccaaggcc tacgtgaagc accccgccga
      421 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      481 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
      541 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
      601 gaagaccatg ggctgggagg cctccaccga gcggatgtac cccgaggacg gcgccctgaa
      661 gggcgagatc aagatgaggc tgaagctgaa ggacggcggc cactacgacg ccgaggtcaa
      721 gaccacctac atggccaaga agcccgtgca gctgcccggc gcctacaaga ccgacatcaa
      781 gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg agcgcgccga
      841 gggccgccac tccaccggcg cctaagaatt cgccagcaca gtggtcgacc ctgtggaatg
      901 tgtgtca
//
