LOCUS       sequence-309257-       18617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Positive control for germline GFP expression from simple array in C.
            elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18617)
  AUTHORS   Aljohani MD, El Mouridi S, Priyadarshini M, Vargas-Velazquez AM, 
            Frokjaer-Jensen C
  TITLE     Engineering rules that minimize germline silencing of transgenes in 
            simple extrachromosomal arrays in C. elegans.
  JOURNAL   Nat Commun. 2020 Dec 9;11(1):6300. doi: 10.1038/s41467-020-19898-0.
  PUBMED    33298957
REFERENCE   2  (bases 1 to 18617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-12-9"; volume: "11(1):6300. doi"; pages: " 
            10.1038/s41467-020-19898-0"
COMMENT     Sequence Label: pCFJ895 - pEXP5605(cbr-unc-119)[Psmu-1 |smu-1
            ...
FEATURES             Location/Qualifiers
     source          1..18617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1491..1502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    3568..3588
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    4584..4608
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(12856..12880)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(12909..12937)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     13685..13705
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(14737..14757)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(14765..14782)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(14765..14781)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(16483..16502)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16602..16624
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(16662..16680)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        16748..16852
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16853..16921,16922..17713)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(17071..17090)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17884..18472
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18373..18392
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gggccctgtc ggattatggg ctcttctttt atcctctact ttctttctag aaaatgcaaa
       61 tgttgagtca gaaattgctg aatggagtac aactgcaatg ggtttagctt atgtttctgg
      121 aacaattact ggtggaattg tgattgaaaa gtaaggttat atttcatttc aataaatgtt
      181 accacagtat aacaaaaaca agttacattg ttcagagtat gtctgaatgt attattatta
      241 ttgtatttgt gcatcagtac tgtagttgta ctgaagaagg gtttaaaata atctcaacgt
      301 ttttaagacg agttagtgag aatacggtcg cagttaggat gtttggacat agttttatta
      361 gataatcgtg aactaactca ctataaaaac ccgctaactc attgaataag atagttttaa
      421 acgttcaaaa tttgaagaca aaattgtatg aataagttta cagagttggt cgacgcaagc
      481 ttctccttct cttcacattt ctcaataatt tagctctaat tgcattcgta ttcttcgcaa
      541 aaatacgtat tctgattgac ccgatgaaat acggatgcct agttgcattg atcatctacg
      601 gttatactta tgggtgaggt ttttcaaata tattttgatt taatcgaata atgaaatttc
      661 agaactggag ttggtccaat ttcgtggttt atatcctctg aactggtacc tcaaaaacat
      721 cgtagtattg ctcaatctgt ggcatattca atgaatactg taatggtggt tatcagtaca
      781 tttacagtat tacctctata ttccgttatt ggtagttatg ctttccttat tctatattca
      841 attccttcat ttataagtat gcttatatta tttcgctact taccggaaac caaaggacga
      901 gagattcatg atattgtgaa tgaacttaaa aaaaaatgaa aacctttgaa aacgttcata
      961 cggccgaaat tttacttgat cgagccaatt gaacttttgt taagacttac agaaaagtga
     1021 gagattttgt tacaaaaaag tgagaaaaaa gcgaaaatga acaggaaaaa gaagaaaaat
     1081 acacccgaag tatcatataa aaattaaaaa gattgtttga cctggcggaa cttttttgca
     1141 aaaactctga atatccatgg cacatgcttc gtgcaaaaca ggatccaaat gaatatcaac
     1201 aagagattct tgaagacgac gagccacttg ctcagagcat tgcttatctc gaatgagacc
     1261 cttgttaaaa tcatttttca aacattatat gacattgtcg aaatgtcctc ctgattccat
     1321 gatggtagca aactcacttc gtctttgagc caatttatcc aagtccttgt aaaaagtatt
     1381 cgaaaattgt taacggataa aatgtttatt ataatatcaa aaacaatttg tcagttgacc
     1441 actttttgat atatttgaca gaaaccggga tgaattggct caaagttagg gcgcccttct
     1501 attacagggt ttctgataac aaaccggtta ttaactccca acaagggatg atttcaattc
     1561 atcatgctca aattgaccca aattaagtta catgacaaat tcatcgccct tttcaactct
     1621 tcttggctca tcatctgtta ttctgttcct attctgtctg caccctatac ccctttgcat
     1681 actctctcgt cattcctttc ttggatagtg tcttcttctc cccagctctg ctacttctat
     1741 gacttgcgcc cgctgctttt ccgctcgttc ctctcctctc tgacgtcttc ttctgcttct
     1801 tttctccttc ctttttccct tctcaatctt cctctttttc ccatttctgt caatcattcg
     1861 aagaagaaga agaagaagac cctcattcat ttcatttttt tttctgtcgg tgtgtgctgc
     1921 tcggttaaga gtgagctctc tattccacgt cttcttcttt ttcttcttcg attcgaatca
     1981 atcactccac caaaacgcat tcgtttttgg gattcacccc gcggttcgca ataggtttct
     2041 ttttcaaatt attagcgtta taaatagaaa aatggtggag tttcaataaa aatgataatt
     2101 tacaaagtgt attttgatta catgtactca aaaaggttga aatttttcat accagttttc
     2161 cggaaatcca tctgatatca ttatcgtatt tttcttttta aaaatgtttt caaaaaaaaa
     2221 caaaatatag ctggttattt ggcaccctct aattaccatt ttcctgtcac accacactct
     2281 tttccttctt ccacttcttt ccgttttcag ccgcttccaa ccaaaccgat atgaaagccg
     2341 agcaacaacg atcgattcca cccggctcgg cgaccttccc gtcgcaggtg agactcagaa
     2401 aactagagaa accgctcaac taactcttga tatccgattt cattcttttc tcttttcttt
     2461 ttgttgaact tttccatatt tccagatgcc acggccacca ccaagcaccg aacaaggaat
     2521 cacaacggaa tcggagcttg cgaagaaagc tcaaatcact ccgaacgacg ttttagcact
     2581 tccgggaatc actcaaggta tcttgctccc tttggatttc agtttaaaac ataaatttag
     2641 gattcttatg ctccccatct gcgaacatct ataacatcga gttcaccaag ttccaaatcc
     2701 gtgatctgga cactgagcaa gtgcttttcg agatcgccaa accggagaac gatcaggaga
     2761 atgatgagtc gccacaggag tcggcaagat acgtgcgtta tagatttgct ccaaactttt
     2821 tgaaactcaa aacggtcgga gcaactgtgg aattcaaagt aggagacatc ccaatccatc
     2881 atttccgaat gatcgaacgt cacttcttca aagatcgcct tctgaagtgt tttgactttg
     2941 aattcggatt ctgtattccg aattcacgaa acaactgtga acatatctat gagttccctc
     3001 aactctctca acaactcagt gagtcattat tctaaaaagt acaattcaaa agactaatct
     3061 ctttttcagt ggacgacatg atcaacaatc caaacgagac tcgttctgac agcttctatt
     3121 tcgtcgataa caaactcgtc atgcacaaca aagccgacta ctcatatgat gcataaatat
     3181 ttaatacaaa aatgttctgg ataattattc tgtcgaatag aaaaaaaact ccaaatgtga
     3241 ttaaattcca ataattcctg tctagtttgt tccttcccct tccccttctc atgttcaatg
     3301 cattcctaag cttttcagtt ccccccttgt ttctatattt tttcgctgtc ctgtcacact
     3361 cgctaaaaac actaatcaca cggaaatctg ttttcaataa aaactccaac tttaactcat
     3421 tttcaatttc aactgaaaga ttttttcatt agagaatgtc tagaactagg cccgggctac
     3481 gtaatacgac tcacttaagg ccttgactag agggtaccag agctcaccta ggcaggaaca
     3541 gctatgacca tgattacgcc aagctatcaa ctttgtatag aaaagttgaa atttttcagg
     3601 cgggaagtgg cttcggtgcg ttcaacaaac gcgcttttga ctcgctagcc ggatccggct
     3661 tcagcggttt tgacaaaaga gctttcgatt cactggctgg acaaggtttt actggatttg
     3721 agaaacgcgc ctttgataca gtggtacggt tttcggtgga aattccagtt tttcgaattt
     3781 tcaataattt tcagagcacg tcaggctttg acgatttcaa attgtgaaga gctcactgat
     3841 atttaaaatt tggttcaatt caagcacata tttttgaggt aagcaactga ttttaatgtt
     3901 gttaatcaaa tgaaaagtga aatttttgga ataatttttt tttgttttca ggtagctcca
     3961 acgaacgaca cacacaagcc cagtccccca gtattcctga cgacagcccc tgctcacaca
     4021 ctttttatta taattccttt tttttcaaac ttttttttca aattttatac tttaatctca
     4081 aaaaacctat tcatacgaac acaagtaccg gtgtgcgtgt gtgagaataa gaatttattt
     4141 taaaaaaata aagaatgtcg tcctgttttt tcatatttat tgaagaaaat ggagctggaa
     4201 aaaaatcaat tgtaaaaaac agaaaaacgt gctgataaaa tgcattgtac gcaaactgtg
     4261 gattttgaga actaacgtag tcaagcaaaa atctctggaa ttttatcgat ttttaacgga
     4321 taaactgttg ttttttgtaa ctgtgcatga aaaaaaatct caggcaaaaa tattttctga
     4381 aaaaaaaaga gtttaaacac aaatgcaagt ccacaaaaaa ctccaatttg aaattcgctc
     4441 caaaaacgca aaacacgcca agcgctcgtg ctggcgcatt tgccgatcgc ttccaaaaag
     4501 gctggcgcac ttctcgtgtc cgaggatatt cgaattttac acgtcatttt cagccgtttt
     4561 tcaccgaatt ttcgctgaaa atgacaagtt tgtacaaaaa agcaggctcg tcatcgattg
     4621 agatcgaatc ttcagagtaa gtttatctaa aaagtttttc attcaaaatg tgtaaaaatt
     4681 catttaaaat aacccaaaaa tcattaatcc tcgatatttt cagtgtaatc cgtctcatcg
     4741 aacaattcct caaagaatcg aatttgcacc gaactctggc gattttgcag gtattttcct
     4801 gcatttttca actgggaaaa tgaaagaaaa tcgataattt caggaagaaa ccaacgtttc
     4861 gctcaataca gtcgactcaa ttgatggatt ttgtaatgaa attacgtctg gaaattggga
     4921 taacgtgttg aaaaccgttc aatcgttgaa attacccgcc aagaaactta tcgatctgta
     4981 tgaacatgta agatatggga aagaaggaaa aaaccgagat tttacttgaa aaattgaatt
     5041 tttcgcggga ttttcaccaa aaattgttga atattcatta tttcacgctg taaaacaaaa
     5101 aaaaaaaaaa tcaaaaacta cgttgaaatc gcgtttttaa gcgaattttc ttcagaattg
     5161 ccagatttta accccaaatt ttgcagtttt taaataaaat ttcacctttt cggctcaaat
     5221 tgtagatttt cctgaaaatt tagtacaaaa aacaatttcc tcgtaaattt ttcaaatttt
     5281 caggtaataa tcgagcttgt ggagctccgt gagctggcga ccgcccgtct cgtcgcccgt
     5341 cagactgatc caatgatttt gctaaaacaa attgatccgg atcgattcgc ccgcctggaa
     5401 tctcttatca accgcccgta tttcgatgga caagaggttt atggagatgt tagtaaagag
     5461 aaacgacgct ccgtaattgc acaaactctt tcatcagaag ttcatgtagt tgctccgtca
     5521 agacttttga gtttacttgg acaatcactt aaatggcaac ttcatcaagg acttcttcca
     5581 cctggaactg caattgattt atttagagga aaagctgcac agaaagagca aattgaagaa
     5641 agatatccga ctatgatggc tagaagcatt aaattctcaa cgagtacgga ttgaaattgc
     5701 tttaaaattt gaaaaattga ttaaaaagtg cattttttaa gctttgaccg acttaaaatt
     5761 agatttctga gcctattttc tgagaattgg aatttttttc attgtgaaag ttcaaagaat
     5821 agcgtgaatg attagaaaat attgtaaaat ttcaattttt tcctataaaa aaggattttt
     5881 ttaggaatca aaaattgcaa aatgatgccc taaaattcga aaataaatat aaaaattggc
     5941 ggtttctcaa aaatctagaa ttccgacctt aattattaat ttttttacaa tatttttttt
     6001 tgaaaaaaat ccagaaaatt tgaatttcgt agtttgtagt ctaggcctct atcaattaaa
     6061 ttttcgattt tttgagtaaa atttcgaatt tactattatt tggaccaaaa ttgtattttt
     6121 ttttcagaat taaatttata aaattttcag aaattaaatt tataaaaaaa aaaaaaaatt
     6181 aaaaaaaaaa aaaaaaaatt aaaaattaaa aattaaaaaa ttaagttttt tttactcaaa
     6241 attttgcact gaaattcgaa aatctaaaaa tccgacctaa agtctgtatt ttttcaacaa
     6301 aaattcagaa aaaactcaaa aacttgtatt ttgtagccag tcaaccactt tctaaaatat
     6361 caaatttgaa tttttcaagc atttttacat tgaaaaatct aatttttcga gtgaaaccac
     6421 ttgaaaatct gaaaattgaa ttaatttctg aactttagag attttttgtc aaaattttag
     6481 ataaagggat ttttaaacaa aaaattgatt ttttaatcga aaaatactgg gatttatggg
     6541 ttttttaaaa gaaaaacggg ggtttgaatg aaaaatcgcc tgaaatctta gaaaattaat
     6601 taaaaaacta tgatttaatt ccaaaaatta ccgaaaatat caaattttcc atttttaaac
     6661 cctaaattct ttcagaaagt tatcccgaat cggcagtttt ctcaccggac gctaattatc
     6721 ttgtttctgg ctcaaaagac ggatttatcg aagtttggaa ttatatgaat ggaaaattga
     6781 gaaaggattt gaaatatcag gtagattttt tagaattttt gggtttttga agtagaaaat
     6841 cataaaaatc tagggttttt atgaattgtt ttgaagaaaa attgcaaaaa ttccacaaaa
     6901 tggaagaaaa ataactttgg aagcgcattt ttcgcaaaaa aaccgaaatt tttgcgtaaa
     6961 atttcaaaat tgcaataaaa attccacaaa catcaaattt cttaaatttt ttataaaaaa
     7021 ttggatggaa acactcttga atttagaaaa aaaatcagtt tttctcatct aaaacttcaa
     7081 aatttcggtg taatcccatt aaaattgcca caaaattcgg aaatttcacc tgaaatagag
     7141 tgaataattt aaaatgttca gaaattcata ttttgtcatt ttaaaagcat taaaacaaat
     7201 caaaaatcta ttttttttgg ttggaaaagt tcaaaattct ggagaaatta cattaaaatt
     7261 ttcgtaaaat atggtaaata aacgaaaatg ttgagaaatt aaagaaaagt tacaattttt
     7321 agcttaaaaa ttcaacattt tgaggaaatg ccacctaaaa aagtgactaa tcgaaaatgt
     7381 tgagaaatta aaattgccac catttattta tataaactac tctaaaatta caattttcat
     7441 gttaaaaatt aataaaaaat ctactttttc caaactacag taaccttacc gtatacctac
     7501 agtacctgaa cattgccccc caccagctcc caacccaata cctcctcaaa aacttacacc
     7561 tcaatttttc ataaactaca gtaaccctac caaaaaagca caaaaaaaaa tctacattca
     7621 ttttccaaca attttcaata ttttcaggcc caagataatc taatgatgat ggatgcagca
     7681 gtgagatgta tatcatttag tcgtgattct gaaatgctcg ccacaggctc aattgacgga
     7741 aaaattaaag tttggaaagt ggaaactggt gattgtcttc gaagatttga tagagctcac
     7801 acgaaaggag tttgtgctgt tcgattctca aaagataatt cacatatttt gtcgggtgga
     7861 aatgatcatg ttgtgaggta cgactacctg cctgcctacc gcctaaattt tttgtgaagt
     7921 ttcttcaaaa aatccagaaa aaaaaacaat tttcatacga ttttttccct taaaattgtg
     7981 aattttcatg ctttttagcc ccaaaagtca ttatttgaga aaaaatttca tacaaaaaag
     8041 ttttgagaaa tacacaattt tttaaatgta attttcaaat tttcaatttt caactagaaa
     8101 attcacaaaa cttgtaaatt ttggaccaaa acatttatac aattactttt tttgaatcta
     8161 ataactacaa taactacaaa ttttgttcag agttcatgga atgaagagcg gaaaatgttt
     8221 gaaggaaatg cgtgggcact cgagttatat tacagatgtc agatattccg atgagggaaa
     8281 tcatattgtg agtttttgaa aagtgcagtt tgtagtctaa ttttcattta ttttcttttt
     8341 aaaaaacgca tcaattttta aatattttgg gacaaaatcc gaaaactgta ctaatttgta
     8401 gtttgtaaaa ttaaaaaaaa aacgcaaaaa aatgttttca aaaatgctta gaataaaaat
     8461 aaaatttcta aaaaattgaa caaaataaac atttaaaaat tcaaaaagtt tgaaaaaatg
     8521 caaagttttt taatgcaaaa aatatttaat gtttaaacaa atttaaaaaa tgttgtaaga
     8581 atatgtttag agacagtttt taaatgttaa attgcaaaaa aaaaaaagca aaaaaaaatt
     8641 tgaaaaatgt aacagaaaat tttaattaaa aaagtcgtgt ttttttcaaa cctgcaattc
     8701 tttgtaattt tatttataaa gagaaacaaa tcaaattgta tattacgaaa aacgatacta
     8761 aaattcgaaa atttgcggtt ttttgcgtaa aaaatacggt tcctgaaaat gggtgtgcac
     8821 ctttaaggag tactgtagtt ctaaactttc attgctgcaa aatttatatc tattttttga
     8881 agttttacaa taaaaataac aattttcatt aaataactac aattaaacag ctaaaaacat
     8941 ataagaaatc gcgagaaaat cgggaaaaaa ttgtagtttg tagtatgtta gccgcaatca
     9001 aatgcatgtt atctctgtat ttcttttctc acacaaatcc cacatatttc tatgtttaca
     9061 cacgtcattt tgtgcacttt ttctgacgtt tcccctccat attgattttg ctttttactg
     9121 tttagaaacg ttgcagaaat ccggaaaaat tgattttttt tctatatata tatatttttt
     9181 tccaagtttc aaatttaaat ttcagaaaaa aaatttagac ttaaaaaaat taaaaatccc
     9241 aaataatttc cagatctcat gttcaacaga tggttcaatt cgtgtttggc atggaaaatc
     9301 gggtgaatgc ctttcaacat tccgtgttgg aagtgaagat tatccaatat taaatgtgat
     9361 tccaattccg aaaagtgatc caccacaaat gattgtatgc aatcgatcca ataccctcta
     9421 cgttgttaat atttctggac aggtaacatg attttgaaaa aaagtttaag aactgaaaaa
     9481 tggaataaaa atatttaaga gcatttttaa atgtaaaatt aacaaaaaaa gcgcctaaga
     9541 atgtttccaa aacagtaaaa aaaaggttta aaaaaatgca aaaaaaaatt taagatgctt
     9601 ttaattacac aaaaaatgaa agtaaaaaaa attaaaatat tgaaaaatgt atatttgttt
     9661 gaaaaggtac atttttcaat gcaaaaaatg tttctaaaaa ttaaaaaaat atgtttaaat
     9721 tgtgtaaagt gtaaattaaa aaataaaatt atgaaaaagt attgtttcat actttttcta
     9781 aattttggtt gaaaaattta aaattccagc tttttattca cattatgtgt tctaaaaatc
     9841 tttccccaat tttcgggacc gttttccagt atatttccat ttttcaaaaa gatttttaac
     9901 tgaaattcat gttttcaatg ctaaaaatca ataaaaaaaa tttcaggtgg ttcgtacgat
     9961 gacgtcagga aagcgtgaga aaggagactt catcaactgc attttatcgc cgaaaggtta
    10021 attttgaccc gaaaattgaa aaaaaaaacc gatttcaaaa ttctaatttt cccatttttt
    10081 tctttaaaat aatttctcct ctggaaaggc tcgtagagca gaaaaagaat taaattagaa
    10141 acggattttt gttcaaattt cccccgtttt ccccttcaaa acttgcaatt cagtttctta
    10201 caggagaatg ggcatacgca attgcagagg acggtgtcat gtactgtttt atggtattat
    10261 cgggtaccct ggaaactact cttcccgtaa gtttagcctc tttttgaact caaattttag
    10321 tttaaaaagc gagaaaaagt acaaggaaaa tgagaaatca agtgcatgaa ttgataagaa
    10381 tagtatggtg agggtgtggt tgatgactag aaaaaatcgt ttttttttaa tcatacacag
    10441 attttcttaa aataaaaaca tcaaaaatgt gtcggactaa ttttgtaaaa taaaatttaa
    10501 tcgaaaaatg aggtaaagtt gaattttttg actccaaaaa cctctatttc tacgctcaat
    10561 tttgtaaaaa aaaattcagg gtaattttta tttcaatgtc tacaaatttt gggggtattg
    10621 ctactgaaaa agtaccgaaa aattattttt ttcgcaaaaa accgttattt ctgaaaaaat
    10681 tctgaaattt attcttcaaa atgttaaaaa tacgcagaat atttcagtac caaaaaacta
    10741 aaaaaaaaaa acaaaatatc acaaaaaaac gaaataaatc gaattgcgaa tttccccaac
    10801 tctcgttaga ttccctattt gaattccttc caaaatgaaa actccccgtc caatcagcgt
    10861 cttcgaacac tccgcccact cattctgatt ggttcaataa tggacggcta tagaaaatct
    10921 atagaaaatc acttgattta tctgaaacca acattaattt cagctgaaaa attagagaaa
    10981 gggctcaaaa ttggcctgaa agttgccaat ttttattaaa aaaattggca actttcaggc
    11041 aaatttttat atattttaaa attctaatta gtcctcattt cgatattcca cgtttccgaa
    11101 ttttgatatt tttttttcaa aatatatatt cctcattaaa aatgttctag ttttttttac
    11161 aacatccctc ttgcctccaa aatttttcga ttttctccca ttcctccctc actcggaaga
    11221 atgttcttcg actgacgtag tggtagtagc agcccataac acatacgtag attaatttta
    11281 tagatagtat agggttttcc tacaccgata ttcattccat tgttcacttt tcgaattgtt
    11341 tcatattgcg attttaaagg cgcatggctt gaaaagtatg aagtttagaa attttacaga
    11401 aaaaacgaaa aaaaaatccg aaaaaaccag aatttcctat ctcctccctc actaacctca
    11461 ataaattccc agtttgaatt ccctccatat tgtgaactgc aaaaccaatc agcgatttgc
    11521 tctgcccact tttcaaccaa tcagataaag tgggcggggt tcgaagtctc tgggggcgtg
    11581 ttacggagct acctatttca tcatgctctt cccagacgtg ggcaacggtt gccattcggc
    11641 aattttttgg tcgacaagtt cggcaaatct gcaaattgcc ggtttgtcga tttgccggaa
    11701 acagccgccc acccctggtt cctccaacat ttttcattaa aaatttgtgt aaaacttcag
    11761 aaaatctctc gactttcctt taaaaagaaa cactaatttc agctgaaaca ctcgaaaatg
    11821 gcctaaaagt tggaaattgc cgattttcag accatttttg agcattttct ctaagatttt
    11881 tgtagtttgt agtctttctg ccaatttgtt taattcagtc ttcccctctc tccaattaaa
    11941 caattcccgt tcacatattt ttttcaatat gaccgcactc ggtacacttt tctgtctgca
    12001 tctctactct ctctctctct ctctcacgga aaattcattt tttttgttgt ttttctcttc
    12061 cgtggtccgt ctagaagatg ctaggaatac taaatatata tatttcgtca attttttttc
    12121 cgcccgcccg tctctcgccc aacgtgctag gcaaccggct tttttttgtt gaaaattttg
    12181 aagatttcga tgaaaaatgt agattttttt tcgggtttct gagctgaaaa tcactgataa
    12241 agctcacaac tttatcagaa aatgtttgat ttttttttca aattttgggt cccgccacga
    12301 tcaacctgat gacggatact gtagtcttga aaattaggtt ttattgaatt tttaggaaaa
    12361 gtttaaatat tcagctgaaa ttcgaaaatt gcagtctgag ccacaatcaa ttttttttca
    12421 aaagcgcccc attcttggat cttgcaacga agatttttta ggttactgta gaaacaaaaa
    12481 attgtaggtt gtagtcatga atatttttcg actttttcaa aataaataag ttcaaataaa
    12541 atggaaattc agcgaaaaaa aagtaaattt ttatgagaaa aatgaagaaa atttaaaaaa
    12601 aaacaaaaaa attttttcgc tataaaaaaa attagaaacg aatattttca agaaaattca
    12661 ataattttga ttgaaaattg gagaaaaaag ttccagtttt ttgctatttt tcttattttt
    12721 tattgaaaaa aaaaatttaa aaatcgataa ttttccaggt gacagaacgt cttccaattg
    12781 gtttggctca tcatccacat cagaatctca tcgcttcata tgcagaagat ggacttttaa
    12841 aattatggac cgataaccca gctttcttgt acaaagtggc gagcaaagga gaagaacttt
    12901 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
    12961 ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
    13021 gcactactgg aaaactacct gttccatggg taagtttaaa catatatata ctaactaacc
    13081 ctgattattt aaattttcag ccaacacttg tcactacttt ctgttatggt gttcaatgct
    13141 tctcgagata cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag
    13201 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtaagt
    13261 ttaaacagtt cggtactaac taaccataca tatttaaatt ttcaggtgct gaagtcaagt
    13321 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
    13381 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
    13441 cagacaaaca aaagaatgga atcaaagttg taagtttaaa catgatttta ctaactaact
    13501 aatctgattt aaattttcag aacttcaaaa ttagacacaa cattgaagat ggaagcgttc
    13561 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag
    13621 acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc
    13681 acatggtcct tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat
    13741 acaaataatt ttccccagat ttccccacga ttttttttga catttttatg aaaattttct
    13801 ctaaaagtta cccctatttt gtttagactg atctcttatt ttatccaatg tttcaaattt
    13861 ttgaaattgc tcgaaaattc caattttcac atatttttgt tgattttttc gccattttcc
    13921 cctgataaat taacttattt ttgcaatttt ttctttcgaa aaaatattgg tttctcatca
    13981 ccatttatcc ttttttttat ggagttaaaa attgttaaaa aaatttaatt ttttttctaa
    14041 ttttagagtc aaaacgggtt cgaatctaga gcgtagctgt acgatttttt ggcgattttt
    14101 gcggttttct ggtgagtttt tttaaagtta tttttttgaa aaaaattctt gaaaataaaa
    14161 ggaaattgtt ttaaaaaaaa atttcaaaga aaaaattttg aaaaaattga aacatttttt
    14221 ttattttgac caaaaatatt gaaaattaat taacattttt ttttggaaag ttaattttta
    14281 atacaaattt tttaaattta ttttacaaaa aaaattgaat ttctgtgaaa aatcagtttt
    14341 ccagtacata atgatttgac caaaaaaaat tgaaaaaaat gattttttaa aattaaaaat
    14401 accataaaat gctgaaaatt cagaaaaata agtaaatttt aaaaaattct ggaaaaacat
    14461 ctaaaaaccc cccaaatata atattttgga cccttttatt ttctgctcga catctcaaat
    14521 atcctccaat ttttcgcttt tttgattaat tgaaagcata gtgggggcag gtgttatagg
    14581 acacccttcc ctccactttt ttggagcatt tttcaaagct cttccgagag cagaagcttc
    14641 tcaatttttc caccccaaaa gtttgaaatt ctgccaaaaa atctcgcatt ttaacaattt
    14701 ttcgagatgt ttcacgtcct tttcattgcg atttcccaac tttattatac atagttgata
    14761 attcactggc cgtcgtttta cactcgagac gtacggtgcg cgcgatgcat tcgaagatct
    14821 gcccactagt gagtcgtatt ataagtgcaa gtaagatcag tgtttgtttc gatatcagtc
    14881 tgtttcgtaa cggtcttctg tataactaca aaaaagaata aaaaaccgta tcacaactga
    14941 gaaaaaagac aaaacttatt gaacagtgtt cctttcaaaa aattattttg taaaaaatcg
    15001 agtttttctc caaaataacg cacgcaatca aaaacaagaa aactaacgtg ttctcctggg
    15061 cgtggaactc cttatcaaga ttaccattat tgtctgtcag aattgatatt tcaattattt
    15121 catatctaga caaacccggg tgtcttgcat ttattagtac tgtgagttta catacaaacg
    15181 aggcttactc agataacata gagtcacctc taattagcaa aaacccattt ctaattagct
    15241 atttcgacca gttctaaagt caccgtaatt agaaatggtt tttgtagctt ttctaattag
    15301 aaatgacccc gagataagtg aaatatggca ttttctgggt tttctagtaa gaaatgatcc
    15361 cgcacgtagt gattccgcgt tacaagccaa attcaaatta aaaaagtata ttacacaatt
    15421 caagttacat tattttcagg aaacaatggc gaagagcaaa gcagagattc aaaaggagga
    15481 aagagacaac agatatgaac gaagacttct tgaaaagaga aagagacatg aggtgagtaa
    15541 ccatgataat aaataatcaa gtaagggcta tagatttata aattctggag ttaacagttt
    15601 cgaaaaaaag tagttttcat atgtgcgaag gaatatcaaa aataggggaa tagcctaaac
    15661 gagtgagaaa attaaatgtt tgttattttt tcaaaaatgt ctgcagggcc gttatccttt
    15721 caaatgcact ctatcaaaca tagatcatat ccatcatatc ttatccagaa tttatatcat
    15781 atccaagatt gagagcgttt caaggaaaat ctcaaacaac catacaattt tgtagatcga
    15841 caacaaggcc tccacttctt catcgatggc ctctgcagca cctgcaccac caagtttgta
    15901 tttttcatat taatataaag tgagccacgc atagtcgttt ttaggatttt tgccagatcg
    15961 atgtcgcgca aacagactgt ttttttttct ttgaaagccg tgcgtgacat gggccacgga
    16021 ttgtaacgcc gcgctggatg gataaaaatt tattcagttt tttagccttt acaaaatatg
    16081 gttcgactac tttctttatt tttacatttt tataggtgct cattgagctg agatattttg
    16141 ggtataatcg gaaaatcgct gattttggtc atttttctgc actaaagatt acacaaaaag
    16201 ttcagtattt agattcattg ttgaaaattg gggatgatct acagatacat atcgccgtgt
    16261 tcaaaaacac acgtgacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
    16321 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
    16381 ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac
    16441 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
    16501 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
    16561 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
    16621 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
    16681 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
    16741 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    16801 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    16861 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    16921 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    16981 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    17041 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    17101 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    17161 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    17221 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    17281 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    17341 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    17401 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    17461 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    17521 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    17581 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    17641 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    17701 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    17761 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    17821 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    17881 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    17941 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    18001 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
    18061 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    18121 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    18181 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    18241 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    18301 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    18361 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    18421 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    18481 caacgcggcc tttttacggt tcctggtctt ttgctggcct tttgctcaca tgttctttcc
    18541 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccag
    18601 cggataacaa tttcaca
//
