LOCUS       sequence-309310-        3080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Single copy or array insertion by MosTI using split unc-119 
            selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   El Mouridi S, Alkhaldi F, Frokjaer-Jensen C
  TITLE     Modular safe-harbor transgene insertion (MosTI) for targeted 
            single-copy and extrachromosomal array integration in C. elegans.
  JOURNAL   G3 (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184.
  PUBMED    35900171
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2022 Jul 28. pii: 6651068. doi: 
            10.1093/g3journal/jkac184."
COMMENT     Sequence Label: pSEM319 - array co-injection fragment mosTI un...
FEATURES             Location/Qualifiers
     source          1..3080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(985..1001)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(985..1001)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(998..1020)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1173..1761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1253..1272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(1878..1902)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2002..2793,2794..2862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2625..2644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2863..2967)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(3066..3080,1..8)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(3080,1..17)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
ORIGIN
        1 gtaaaacgac ggccagtcat gcataatccg cacgcatctg gaataaggaa gtgccattcc
       61 gcctgacctg atatcaagag ttagttgagc ggaactaatc tgatttaaat tttcagatgc
      121 cacgtccacc tccaagtaca gaacaaggta tcaccaccga atccgaactg gccaaaaaag
      181 cgcagatcac cccgaacgac gtgctggccc tgccaggcat tacccaaggc ttcctgtgca
      241 gcccaagcgc gaacatttat aacattgagt ttaccaagtt tcagatccgc gatctggata
      301 ccgagcaggt aagtttaaac atatatatac taactaaccc tgattattta aattttcagg
      361 tgctgtttga gattgcgaag ccggaaaacg accaggagaa cgatgaatcc ccgcaagaaa
      421 gcgcccgcta cgtgcgctac cgctttgcgc cgaactttct caaactcaaa accgtgggag
      481 cgaccgtcga atttaaggta agtttaaaca gttcggtact aactaaccat acatatttaa
      541 attttcaggt gggcgatatt ccaattcatc attttcgtat gattgaacgc catttcttta
      601 aagatcgcct cctgaagtgc tttgatttcg aatttggatt ttgcattcca aacagccgta
      661 acaactgcga acatatctat gagtttccgc aactgagcca gcagctcatg gatgatatga
      721 tcaacaaccc gaacgaaacc cgtagcgaca gcttttactt tgtcgataac aaactcgtga
      781 tgcacaacaa agcggactat agctacgatg cgtaaatctt cattgttgag tttatcttgt
      841 tgatttttga ataaattatc aactctttac tttttaatgg gttatgaaat aaataaacat
      901 tgaaaactga taaacaacgt tcatctctag gctaggtgga ggctcagtga tgataagtct
      961 gcgatggtgg atgcatgtgt catggtcata gctgtttcct gtgtgaaatt gttatccgct
     1021 cagagggcac aatcctattc cgcgctatcc gacaatctcc aagacattag gtggagttca
     1081 gttcggcgta tggcatatgt cgctggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1141 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1201 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1261 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1321 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1381 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1441 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1501 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1561 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1621 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1681 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1741 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcta
     1801 ttcaacaaag ccgccgtccc gtcaagtcag cgtaaatggg tagggggctt caaatcgtcc
     1861 tcgtgatacc aattcggagc ctgctttttt gtacaaactt gttgataatg gcaattcaag
     1921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     1981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     2041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     2101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagagccac gctcaccggc
     2161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     2221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     2281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     2341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     2401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     2461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     2521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     2581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     2641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     2701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     2761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     2821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     2881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     2941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccag atacctgaaa
     3001 caaaacccat cgtacggcca aggaagtctc caataactgt gatccaccac aagcgccagg
     3061 gttttcccag tcacgacgtt
//
