LOCUS       sequence-309386-         435 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Gag P6 from HIV-1 HXB2 strain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Ramaraju B, Nelson SL, Zheng W, Ghirlando R, Deshmukh L
  TITLE     Quantitative NMR Study of Insulin-Degrading Enzyme Using 
            Amyloid-beta and HIV-1 p6 Elucidates Its Chaperone Activity.
  JOURNAL   Biochemistry. 2021 Aug 3. doi: 10.1021/acs.biochem.1c00342.
  PUBMED    34342986
REFERENCE   2  (bases 1 to 435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2021 Aug 3. doi: 10.1021/acs.biochem.1c00342."
COMMENT     Sequence Label: pET-11a HIV-1 P6
FEATURES             Location/Qualifiers
     source          1..435
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             172..189
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             190..201
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             241..258
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             259..279
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcagt ataaactggc tttaaatggc aagactttaa aaggcgagac caccaccgaa
       61 gcagttgatg ccgccaccgc cgagaaagtg tttaaacagt acgccaatga taatggcgtg
      121 gatggcgaat ggacctatga tgacgccacc aaaaccttca ccgtgaccga actggttccg
      181 cgcggtagca ttgaaggtcg cattcgcgag aatttaaagg atagcggtct gtttctggaa
      241 caccatcacc accaccatga gaatttatac ttccagagtc gtccggaacc gacagcaccg
      301 ccggaagaaa gctttcgtag tggcgtggaa accactaccc cgccgcagaa acaagaaccg
      361 atcgataaag agctgtatcc gctgacctct ttacgctctt tatttggtaa cgatccgagc
      421 agccagtaag gatcc
//
