LOCUS       sequence-309403-        5355 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 
            enhancer from Autographa californica nuclear polyhedris virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Russell TA, Ayaz A, Davidson AD, Fernandez-Sesma A, Maringer K
  TITLE     Imd pathway-specific immune assays reveal NF-kappaB stimulation by 
            viral RNA PAMPs in Aedes aegypti Aag2 cells.
  JOURNAL   PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0008524. doi: 
            10.1371/journal.pntd.0008524.
  PUBMED    33591970
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS Negl 
            Trop Dis."; date: "2021-02-16"; volume: "15(2):e0008524. doi"; 
            pages: " 10.1371/journal.pntd.0008524"
COMMENT     Sequence Label: pKM28
FEATURES             Location/Qualifiers
     source          1..5355
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..483
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        487..1078
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             2818..2841
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2872..3179
                     /label=IE1 terminator
                     /note="terminator of the ie1 gene from the baculovirus 
                     Autographa californica"
     promoter        3209..3313
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3314..3382,3383..4174)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3532..3551)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4345..4933
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4834..4853
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5087..5104
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5221..5242
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5257..5262,5263..5280,5281..5287)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5295..5311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5300..5322
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5319..5335
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5319..5335
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
       61 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
      121 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
      181 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
      241 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
      301 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
      361 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
      421 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
      481 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
      541 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
      601 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
      661 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
      721 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
      781 tcacgtacgt ccctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
      841 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
      901 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
      961 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
     1021 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
     1081 accatgggac caaacgacaa caacgttatg gaagatgatc ccagcctgcc ggatctgccc
     1141 aacaacctca tagtaacgga tgacattttg gacctaatca ttgatataat cgaccaagaa
     1201 atgccaccaa cagcgcaaga aagaccgcac gtcgtcatca ccgagcagcc ccagtcgaaa
     1261 gcgctgcggt tccggtatga gtgcgaaggc cgttccgccg ggtcgatccc cggcgtccac
     1321 agcaccccgg agcagaaaac cttccccggg atcgaaatcc ggggctacaa gggccgagcg
     1381 gtggtggtgg tgtcctgcgt taccaaggat ccgccctacc ggccccatcc gcacaatctg
     1441 gtcggcaagg agggctgcaa gaagggagtc tgcacggtcg agatcaacag cagcaccatg
     1501 agctacacct tcagcaacct gggcatccag tgcgtcaaga agaaggacat cgaagaagcg
     1561 ctccgtctgc gggaggaaat tcgggtggac ccgttcaaaa ctggatacgg tcacgccagg
     1621 cagcctgcca ccatcgatct caacgccatc cgtctgtgct tccaggtgtt cctggagggt
     1681 caacagcggg gccggttcac ggaacccctg cagccggtgg tgtccgatgt gatctacgat
     1741 aagaaagcca tgtccgatct ggtgatctgt aagttaagtg acgtaaccgc tccggtagcc
     1801 ggtggcagag agattatttt gctgtgtgag aaggtcgcca aagaggacat cgccgtgcgt
     1861 ttctacgagg aacagcacgg taacatcgta tgggaggaat acggcgagtt ccagcatacc
     1921 aatgtgcaca aacaggtggc gatctgcttc cggacgcccc gataccgaac gctggaggtg
     1981 gaacatcctg tcatggtgaa cattcaactg aagaggccat cggacggtgc taccagcgaa
     2041 ccgctgccat tcgagttgac gccccttgac tcagccaaac gaaagaaacg caagcctaac
     2101 acctgggggg acaccccaga acacatgcca gaagcagccc gccatccgta ctacgcaacg
     2161 ttgggcaatg tgccgggaga agctatcaaa acggaaccta gagattcacc gacgcacccg
     2221 tttaacttca ttggcaacta ccgtcaaccg aacgagtccc cgtccccgat cgagcgatcc
     2281 ccactttacg gcaacaccac cccagtcgga caaacgtctc cctacaatgc cggcagaacc
     2341 agcgtgactc caacaccggg cggctatcaa cccaatgtga tgaatccaga tttcctgctg
     2401 gggggcaccc aacagttcca gaatcagtac gcctttccca cactgccccc gaccaatggc
     2461 caacccatgt acctgcagca accccaacag caacaacaac aacagttcca accacccgcc
     2521 aacatgaaca accaacagca gttcaacgtg agcaatctca tagaccctca ggatcaactg
     2581 cctcagccgg gacccagtgg cgttcaggcg gttccttcgt ttcaaccctc gggcagcata
     2641 ggcgagatca acatcagcag cccctggcta accatggact cgtcggagct gaaatcgctg
     2701 atggtccaca ccctgtccga tatgaagcag gaacagcaac ggatccaaca ggaggccgaa
     2761 accctctcgg acagtttcga caggttatca acgaacctca acaacctgaa caaaccggat
     2821 tacaaggatg acgatgacaa gtaactcgag cactaagtga ttaacctcag gttatacata
     2881 tattttgaat ttaattaatt atacatatat tttatattat ttttgtcttt tattatcgag
     2941 gggccgttgt tggtgtgggg ttttgcatag aaataacaat gggagttggc gacgttgctg
     3001 cgccaacacc acctcccttc cctcctttca tcatgtatct gtagataaaa taaaatatta
     3061 aacctaaaaa caagaccgcg cctatcaaca aaatgatagg cattaacttg ccgctgacgc
     3121 tgtcactaac gttggacgat ttgccgacta aaccttcatc gcccagtaac caatctagac
     3181 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3241 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3301 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3361 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3421 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3481 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3541 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3601 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3661 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3721 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3781 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3841 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3901 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3961 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     4021 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     4081 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     4141 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     4201 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4261 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4321 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4381 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4441 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     4501 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4561 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4621 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4681 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4741 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4801 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4861 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4921 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4981 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     5041 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     5101 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     5161 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     5221 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     5281 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     5341 ttacgaattc ccggg
//
