LOCUS       sequence-309421-        1528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GAVPO (VIVID transactivator) expressed under the CMV promoter in the
            pcDNA3.1 backbone. AKA 133.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Rodriques SG, Chen LM, Liu S, Zhong ED, Scherrer JR, Boyden ES, Chen
            F
  TITLE     RNA timestamps identify the age of single molecules in RNA 
            sequencing.
  JOURNAL   Nat Biotechnol. 2020 Oct 19. pii: 10.1038/s41587-020-0704-z. doi: 
            10.1038/s41587-020-0704-z.
  PUBMED    33077959
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2020 Oct 19. pii: 10.1038/s41587-020-0704-z. doi: 
            10.1038/s41587-020-0704-z."
COMMENT     Sequence Label: pcDNA3.1-GAVPO
FEATURES             Location/Qualifiers
     source          1..1528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             677..703
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1133..1504
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             1505..1528
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 caccatgaag ttgttgagct caatcgaaca agcgtgtgac atatgtcgcc ttaagaaact
       61 caaatgttct aaagaaaagc ctaagtgcgc gaaatgtttg aaaaacaatt gggaatgccg
      121 ctattctcct aagacaaagc gctctccgct cacaagagcg caccttacag aggttgagtc
      181 ccgcctcgag cgcttggaga ggtccattgc aacgagaagt catacactct acgcccccgg
      241 agggtatgac ataatgggtt acctcatcca aataatgaaa agaccgaatc ctcaagtaga
      301 gcttggaccg gtcgacacat ccgtagcact catactttgc gatttgaaac aaaaggacac
      361 tccgatagtg tatgcgtcag aggcgtttct ttatatgaca gggtactcca acgccgaggt
      421 gttgggacga aattgccgat ttcttcagtc cccagatgga atggttaagc ccaagtctac
      481 tagaaagtat gttgacagca atacgattaa cactatgaga aaggccattg atcggaatgc
      541 cgaggtgcag gttgaggtag tcaacttcaa gaagaatggg caaagattcg tcaacttcct
      601 tacaatgatt cccgttcgcg atgagacggg cgagtatcga tactcaatgg gtttccagtg
      661 tgaaaccgaa ttgcaatatc cttatgatgt acccgattac gcagaattcc aataccttcc
      721 agacacggat gatcgccatc gaatcgaaga gaagagaaaa cgcacctacg agacgttcaa
      781 atctattatg aagaaatctc ccttcagtgg gccgacggac ccaaggccgc caccgcgaag
      841 gatagccgtt ccatcaagaa gctcagcttc tgtacccaaa cccgccccac agccttaccc
      901 ctttacttcc tccctctcca ctatcaacta cgatgagttc cccacgatgg tttttccttc
      961 aggacagata tcccaagcga gcgccctcgc accagcccca ccacaagtgc ttcctcaggc
     1021 ccctgcgcct gctccggcac cggcgatggt gagcgctctg gctcaagctc ccgcgccagt
     1081 ccctgttttg gcaccagggc cacctcaggc agtggctccg cccgctccaa aacctactca
     1141 agcgggcgaa ggaactctga gcgaggcgct cctgcagctt caatttgatg acgaagatct
     1201 cggcgcactc ctcggtaatt caacggaccc cgctgttttc actgacctgg caagcgtgga
     1261 taactctgaa ttccaacagc tccttaacca gggcataccg gtcgcgcctc atacaactga
     1321 accaatgctg atggaatatc cggaggcaat aaccagactt gtgacggggg cgcagcgacc
     1381 gcctgatcca gcacccgcac cgcttggggc gcctggcttg cccaatggac tcctttctgg
     1441 cgacgaggac ttttccagca tcgcagacat ggacttttct gcactccttt ctcagatttc
     1501 ctcagattac aaagacgacg atgataag
//
