LOCUS       sequence-30945-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Hollopeter G, Taylor J, Harris TW, Nix 
            P, Lofgren R, Prestgard-Duke M, Bastiani M, Moerman DG, Jorgensen EM
  TITLE     Targeted gene deletions in C. elegans using transposon excision.
  JOURNAL   Nat Methods. 2010 Jun;7(6):451-3. doi: 10.1038/nmeth.1454. Epub 2010
            Apr 25.
  PUBMED    20418868
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1454"; journalName: "Nat Methods"; date: "2010-06"; 
            volume: "7"; issue: "6"; pages: "451-3"
COMMENT     Sequence Label: pRL8
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    72..96
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(242..253)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat gagcaatgct
       61 tttttataat gccaactttg tacaaaaaag caggcttgag ccaatttatc caagtccttg
      121 taaaaagtat tcgaaaattg ttaacggata aaatgtttat tataatatca aaaacaattt
      181 gtcagttgac cactttttga tatatttgac agaaaccggg atgaattggc tcaaagttag
      241 ggcgcccttc tattacaggg tttctgataa caaaccggtt attaactccc aacaagggat
      301 gatttcaatt catcatgctc aaattgaccc aaattaagtt acatgacaaa ttcatcgccc
      361 ttttcaactc ttcttggctc atcatctgtt attctgttcc tattctgtct gcaccctata
      421 cccctttgca tactctctcg tcattccttt cttggatagt gtcttcttct ccccagctct
      481 gctacttcta tgacttgcgc ccgctgcttt tccgctcgtt cctctcctct ctgacgtctt
      541 cttctgcttc ttttctcctt cctttttccc ttctcaatct tcctcttttt cccatttctg
      601 tcaatcattc gaagaagaag aagaagaaga ccctcattca tttcattttt ttttctgtcg
      661 gtgtgtgctg ctcggttaag agtgagctct ctattccacg tcttcttctt tttcttcttc
      721 gattcgaatc aatcactcca ccaaaacgca ttcgtttttg ggattcaccc cgcggttcgc
      781 aataggtttc tttttcaaat tattagcgtt ataaatagaa aaatggtgga gtttcaataa
      841 aaatgataat
//
