LOCUS       sequence-309456-       13227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Human ACE2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13227)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 13227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Bla-Ak-ACE2
FEATURES             Location/Qualifiers
     source          1..13227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2145..3323
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2376..3314
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3271..3291
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3371..3488
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6031..6582
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6175..6192)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6402..6421
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6585..6983
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     complement(7028..7044)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7029..7045)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    7058..7646
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7111..7131)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7529..7540)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(7790..7823)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             7843..8023
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(8048..8066)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8049..8066)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8356..8375)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8589..8608)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8708..8730
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8768..8786)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8854..8958
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8959..9027,9028..9819)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9177..9196)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9990..10578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10479..10498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10732..10749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10819..10839)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10824..11153
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11004..11139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          12287..12352
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12482..12502
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12927..13061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12964..12983)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13018..13037
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgatg tcgacgataa gctttgcaaa gatggataaa gttttaaaca gagaggaatc
     2101 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc
     2161 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt
     2221 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc
     2281 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg
     2341 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg
     2401 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc
     2461 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt
     2521 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc
     2581 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag
     2641 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa
     2701 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg
     2761 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa
     2821 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt
     2881 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa
     2941 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag
     3001 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt
     3061 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt
     3121 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg
     3181 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg
     3241 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt
     3301 ttcttccatt tcaggtgtcg tgaggaattt cgacatttaa atttaattaa tctcgacggt
     3361 atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
     3421 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
     3481 aaaattttat cgatcacgag actagcctcg aggtttaaac ccgggagcgc caccatgtca
     3541 agctcttcct ggctccttct cagccttgtt gctgtaactg ctgctcagtc caccattgag
     3601 gaacaggcca agacattttt ggacaagttt aaccacgaag ccgaagacct gttctatcaa
     3661 agttcacttg cttcttggaa ttataacacc aatattactg aagagaatgt ccaaaacatg
     3721 aataatgctg gggacaaatg gtctgccttt ttaaaggaac agtccacact tgcccaaatg
     3781 tatccactac aagaaattca gaatctcaca gtcaagcttc agctgcaggc tcttcagcaa
     3841 aatgggtctt cagtgctctc agaagacaag agcaaacggt tgaacacaat tctaaataca
     3901 atgagcacca tctacagtac tggaaaagtt tgtaacccag ataatccaca agaatgctta
     3961 ttacttgaac caggtttgaa tgaaataatg gcaaacagtt tagactacaa tgagaggctc
     4021 tgggcttggg aaagctggag atctgaggtc ggcaagcagc tgaggccatt atatgaagag
     4081 tatgtggtct tgaaaaatga gatggcaaga gcaaatcatt atgaggacta tggggattat
     4141 tggagaggag actatgaagt aaatggggta gatggctatg actacagccg cggccagttg
     4201 attgaagatg tggaacatac ctttgaagag attaaaccat tatatgaaca tcttcatgcc
     4261 tatgtgaggg caaagttgat gaatgcctat ccttcctata tcagtccaat tggatgcctc
     4321 cctgctcatt tgcttggtga tatgtggggt agattttgga caaatctgta ctctttgaca
     4381 gttccctttg gacagaaacc aaacatagat gttactgatg caatggtgga ccaggcctgg
     4441 gatgcacaga gaatattcaa ggaggccgag aagttctttg tatctgttgg tcttcctaat
     4501 atgactcaag gattctggga aaattccatg ctaacggacc caggaaatgt tcagaaagca
     4561 gtctgccatc ccacagcttg ggacctgggg aagggcgact tcaggatcct tatgtgcaca
     4621 aaggtgacaa tggacgactt cctgacagct catcatgaga tggggcatat ccagtatgat
     4681 atggcatatg ctgcacaacc ttttctgcta agaaatggag ctaatgaagg attccatgaa
     4741 gctgttgggg aaatcatgtc actttctgca gccacaccta agcatttaaa atccattggt
     4801 cttctgtcac ccgattttca agaagacaat gaaacagaaa taaacttcct gctcaaacaa
     4861 gcactcacga ttgttgggac tctgccattt acttacatgt tagagaagtg gaggtggatg
     4921 gtctttaaag gggaaattcc caaagaccag tggatgaaaa agtggtggga gatgaagcga
     4981 gagatagttg gggtggtgga acctgtgccc catgatgaaa catactgtga ccccgcatct
     5041 ctgttccatg tttctaatga ttactcattc attcgatatt acacaaggac cctttaccaa
     5101 ttccagtttc aagaagcact ttgtcaagca gctaaacatg aaggccctct gcacaaatgt
     5161 gacatctcaa actctacaga agctggacag aaactgttca atatgctgag gcttggaaaa
     5221 tcagaaccct ggaccctagc attggaaaat gttgtaggag caaagaacat gaatgtaagg
     5281 ccactgctca actactttga gcccttattt acctggctga aagaccagaa caagaattct
     5341 tttgtgggat ggagtaccga ctggagtcca tatgcagacc aaagcatcaa agtgaggata
     5401 agcctaaaat cagctcttgg agataaagca tatgaatgga acgacaatga aatgtacctg
     5461 ttccgatcat ctgttgcata tgctatgagg cagtactttt taaaagtaaa aaatcagatg
     5521 attctttttg gggaggagga tgtgcgagtg gctaatttga aaccaagaat ctcctttaat
     5581 ttctttgtca ctgcacctaa aaatgtgtct gatatcattc ctagaactga agttgaaaag
     5641 gccatcagga tgtcccggag ccgtatcaat gatgctttcc gtctgaatga caacagccta
     5701 gagtttctgg ggatacagcc aacacttgga cctcctaacc agccccctgt ttccatatgg
     5761 ctgattgttt ttggagttgt gatgggagtg atagtggttg gcattgtcat cctgatcttc
     5821 actgggatca gagatcggaa gaagaaaaat aaagcaagaa gtggagaaaa tccttatgcc
     5881 tccatcgata ttagcaaagg agaaaataat ccaggattcc aaaacactga tgatgttcag
     5941 acctcctttt agggatccat atgcggaccg tacgtactag tggccacggc tgatcatacg
     6001 ggctgcagga attccgcccc acccccccta acgttactgg ccgaagccgc ttggaataag
     6061 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     6121 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     6181 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     6241 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     6301 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     6361 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     6421 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     6481 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
     6541 ccacggggac gtggttttcc tttgaaaaac acgatgataa taccatggcc aagcctttgt
     6601 ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc atccccatct
     6661 ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc ttcactggtg
     6721 tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg ggcactgctg
     6781 ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag aacaggggca
     6841 tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct gggatcaaag
     6901 ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa ttgctgccct
     6961 ctggttatgt gtgggagggc taactagcta gtcgagctca acttcgaatt cgatatcaag
     7021 cttatcgcga taccgtcgac ctcgagggaa ttccgataat caacctctgg attacaaaat
     7081 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     7141 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     7201 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     7261 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     7321 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     7381 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     7441 gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct
     7501 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     7561 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     7621 gatctccctt tgggccgcct ccccgcatcg ggaattcgag ctcggtacct ttaagaccaa
     7681 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     7741 ggctaattca ctcccaacga agacaagatg ggatcaattc accatgggaa taacttcgta
     7801 tagcatacat tatacgaagt tatgctgctt tttgcttgta ctgggtctct ctggttagac
     7861 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7921 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7981 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagcatcta gaattaattc
     8041 cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat acataaggtt atgtattaat
     8101 tgtagccgcg ttctaacgac aatatgtaca agcctaattg tgtagcatct ggcttactga
     8161 agcagaccct atcatctctc tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct
     8221 tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt cagcgaacca atcagcaggg
     8281 tcatcgctag ccagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     8341 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     8401 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     8461 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     8521 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc
     8581 actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca
     8641 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     8701 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga
     8761 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
     8821 tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     8881 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     8941 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     9001 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     9061 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     9121 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     9181 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     9241 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     9301 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     9361 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     9421 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     9481 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     9541 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     9601 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     9661 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     9721 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     9781 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     9841 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     9901 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     9961 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
    10021 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
    10081 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
    10141 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
    10201 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
    10261 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
    10321 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
    10381 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
    10441 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
    10501 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
    10561 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
    10621 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
    10681 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
    10741 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
    10801 attcattaat gcagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    10861 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
    10921 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    10981 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
    11041 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
    11101 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagcttg
    11161 gacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
    11221 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
    11281 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
    11341 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
    11401 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
    11461 cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata
    11521 ccgtttgtat ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg
    11581 cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct ggtggatacc ggtggtactg
    11641 cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg
    11701 ctggtcgtcc gctggttgat gactatgttg ttgatatccc gcaagatacc tggattgaac
    11761 agccgtggga tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac
    11821 gcctggcact gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta
    11881 agtattctgg aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc
    11941 cgctttaaac atcctgaaac ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg
    12001 cctgtgcagt cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt
    12061 ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc tcgacgtcca ggcacgtatt
    12121 gtgatgagcg atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgt
    12181 ggcggcaact ggatttatga gtgggccccg gatctttgtg aaggaacctt acttctgtgg
    12241 tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt
    12301 tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac
    12361 ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg
    12421 ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc
    12481 tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt
    12541 tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa
    12601 ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag
    12661 taggcataac agttataatc ataacatact gttttttctt actccacaca ggcatagagt
    12721 gtctgctatt aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg
    12781 ggttaataag gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca
    12841 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    12901 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    12961 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    13021 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
    13081 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
    13141 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
    13201 gttaaatatg tactacaaac ttagtag
//
