LOCUS       sequence-30946-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Hollopeter G, Taylor J, Harris TW, Nix 
            P, Lofgren R, Prestgard-Duke M, Bastiani M, Moerman DG, Jorgensen EM
  TITLE     Targeted gene deletions in C. elegans using transposon excision.
  JOURNAL   Nat Methods. 2010 Jun;7(6):451-3. doi: 10.1038/nmeth.1454. Epub 2010
            Apr 25.
  PUBMED    20418868
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1454"; journalName: "Nat Methods"; date: "2010-06"; 
            volume: "7"; issue: "6"; pages: "451-3"
COMMENT     Sequence Label: pCFJ66
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    71..95
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(244..255)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgatg agcaatgctt
       61 ttttataatg ccaactttgt acaaaaaagc aggctctttg agccaattta tccaagtcct
      121 tgtaaaaagt attcgaaaat tgttaacgga taaaatgttt attataatat caaaaacaat
      181 ttgtcagttg accacttttt gatatatttg acagaaaccg ggatgaattg gctcaaagtt
      241 agggcgccct tctattacag ggtttctgat aacaaaccgg ttattaactc ccaacaaggg
      301 atgatttcaa ttcatcatgc tcaaattgac ccaaattaag ttacatgaca aattcatcgc
      361 ccttttcaac tcttcttggc tcatcatctg ttattctgtt cctattctgt ctgcacccta
      421 tacccctttg catactctct cgtcattcct ttcttggata gtgtcttctt ctccccagct
      481 ctgctacttc tatgacttgc gcccgctgct tttccgctcg ttcctctcct ctctgacgtc
      541 ttcttctgct tcttttctcc ttcctttttc ccttctcaat cttcctcttt ttcccatttc
      601 tgtcaatcat tcgaagaaga agaagaagaa gaccctcatt catttcattt ttttttctgt
      661 cggtgtgtgc tgctcggtta agagtgagct ctctattcca cgtcttcttc tttttcttct
      721 tcgattcgaa tcaatcactc caccaaaacg cattcgtttt tgggattcac cccgcggttc
      781 gcaataggtt tctttttcaa attattagcg ttataaatag aaaatggtgg agtttcaata
      841 aaaatgataa tttacaaagt gnattttgat tacatgtact caaaaggttg aaattttcat
      901 accagtttc
//
