LOCUS       sequence-309473-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Specific gRNA against mouse IG-DMR cloned in the pX330 backbone 
            (Addgene Number 42230). Deletion of IG-DMR in mouse ESC..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Aizawa E, Dumeau CE, Freimann R, Di Minin G, Wutz A
  TITLE     Polyploidy of semi-cloned embryos generated from parthenogenetic 
            haploid embryonic stem cells.
  JOURNAL   PLoS One. 2020 Sep 10;15(9):e0233072. doi: 
            10.1371/journal.pone.0233072. eCollection 2020.
  PUBMED    32911495
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2020-09-10"; volume: "15(9):e0233072. doi"; pages: " 
            10.1371/journal.pone.0233072. eCollection 2020"
COMMENT     Sequence Label: pX330_gRNA_IG-DMR-1
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     120..139
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        218..293
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        390..675
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ttagagagat aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt
       61 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
      121 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
      181 ggaaaggacg aaacacccgt acagagctcc atggcacgtt ttagagctag aaatagcaag
      241 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttg
      301 ttttagagct agaaatagca agttaaaata aggctagtcc gtttttagcg cgtgcgccaa
      361 ttctgcagac aaatggctct agaggtaccc gttacataac ttacggtaaa tggcccgcct
      421 ggctgaccgc ccaacgaccc ccgcccattg acgtcaatag taacgccaat agggactttc
      481 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
      541 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
      601 tgtgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
      661 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
      721 ccctccccac ccccaatttt gtatttattt attttttaat tat
//
