LOCUS       sequence-309479-        9171 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible expression of HO endonuclease in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9171)
  AUTHORS   Gnugge R, Symington LS
  TITLE     Efficient DNA double-strand break formation at single or multiple 
            defined sites in the Saccharomyces cerevisiae genome.
  JOURNAL   Nucleic Acids Res. 2020 Oct 14. pii: 5923424. doi: 
            10.1093/nar/gkaa833.
  PUBMED    33053188
REFERENCE   2  (bases 1 to 9171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2020 Oct 14. pii: 5923424. doi: 10.1093/nar/gkaa833."
COMMENT     Sequence Label: pRG710_lexO-HO_LexA-TF_HIS3MX
FEATURES             Location/Qualifiers
     source          1..9171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1574..1585
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2189..2212
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      2262..2511
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     CDS             join(3181..3432,3433..3786)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     3724..3745
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
     CDS             3805..4740
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3853..4743
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     terminator      5207..5456
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(5619..5637)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5658..5674)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5658..5674)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5671..5693)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5682..5698
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(5932..5949)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6103..6691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6183..6202)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6870..7661,7662..7730))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7493..7512
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7731..7835)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             8133..8783
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe his5"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=S. pombe his5
                     /note="yeast auxotrophic marker; corresponds to S. 
                     cerevisiae HIS3"
                     /translation="MRRAFVERNTNETKISVAIALDKAPLPEESNFIDELITSKHANQK
                     GEQVIQVDTGIGFLDHMYHALAKHAGWSLRLYSRGDLIIDDHHTAEDTAIALGIAFKQA
                     MGNFAGVKRFGHAYCPLDEALSRSVVDLSGRPYAVIDLGLKREKVGELSCEMIPHLLYS
                     FSVAAGITLHVTCLYGSNDHHRAESAFKSLAVAMRAATSLTGSSEVPSTKGVL"
     primer_bind     9122..9144
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9136..9153
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9137..9153
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(9160..9171,1..8)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        join(9160..9171,1..7)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctatagggcg aattggagaa acagaataat atataaacct gtataatata accttgaaga
       61 ctatatttct tttcgcggcc gcctataggg cgaattggag ctccctaggt gctgtatata
      121 ctcacagcat aactgtatat acacccaggg tctaggtgct gtatatactc acagcataac
      181 tgtatataca cccagggtct aggtgctgta tatactcaca gcataactgt atatacaccc
      241 agggtctagg tgctgtatat actcacagca taactgtata tacacccagg gtctagagca
      301 tgtgctctgt atgtatataa aactcttgtt ttcttctttt ctcttctttc cttatacatt
      361 aggacctttg cagcataaat tactatactt ctatactagt ggatcccccg ggctgcagga
      421 attcatgctt tctgaaaaca cgactattct gatggctaac ggtgaaatta aagacatcgc
      481 aaacgtcacg gctaactctt acgttatgtg cgcagatggc tccgctgccc gcgtcataaa
      541 tgtcacacag ggctatcaga aaatctataa tatacagcaa aaaaccaaac acagagcttt
      601 tgaaggtgaa cctggtaggt tagatcccag gcgtagaaca gtttatcagc gtcttgcatt
      661 acaatgtact gcaggtcata aattgtcagt cagggtccct accaaaccac tgttggaaaa
      721 aagtggtaga aatgccacca aatataaagt gagatggaga aatctgcagc aatgtcagac
      781 gcttgatggt aggataataa taattccaaa aaaccatcat aagacattcc caatgacagt
      841 tgaaggtgag tttgccgcaa aacgcttcat agaagaaatg gagcgctcta aaggagaata
      901 tttcaacttt gacattgaag ttagagattt ggattatctt gatgctcaat tgagaatttc
      961 tagctgcata agatttggtc cagtactcac aggaaatggt gttttatcta aatttctcac
     1021 tggacgtagt gaccttgtaa ctcctgctgt aaaaagtatg gcttggatgc ttggtctgtg
     1081 gttaggtgac ggtacaacaa aagagccaga aatctcagta gatagcttgg atcctaagct
     1141 aatggagagt ttaagagaaa atgcgaaaat ctggggtctc taccttacgg tttgtgacga
     1201 tcacgttccg ctacgtgcca aacatgtaag gcttcattat ggagatggtc cagatgaaaa
     1261 caggaagaca aggaatttga ggaaaaataa tccattctgg aaagctgtca caattttaaa
     1321 gtttaaaagg gatcttgatg gagagaagca aatccctgaa tttatgtacg gcgagcatat
     1381 agaagttcgt gaagcattct tagccggctt gatcgactca gatgggtacg ttgtgaaaaa
     1441 gggcgaaggc cctgaatctt ataaaatagc aattcaaact gtttattcat ccattatgga
     1501 cggaattgtc catatttcaa gatctcttgg tatgtcagct actgtgacga ccaggtcagc
     1561 tagggaggaa atcattgaag gaagaaaagt ccaatgtcaa tttacatacg actgtaatgt
     1621 tgctggggga acaactttac agaatgtttt gtcatattgt cgaagtggtc acaaaacaag
     1681 agaagttccg ccaattataa aaagggaacc cgtatatttc agcttcacgg atgatttcca
     1741 gggtgagagt actgtatatg ggcttacgat agaaggccat aaaaatttct tgcttggcaa
     1801 caaaatagaa gtgaaatcat gtcgaggctg ctgtgtggga gaacagcata aaatatcaca
     1861 aaaaaagaat ctaaaacact gtgttgcttg tcccagaaag ggaatcaagt atttttataa
     1921 agattggagt ggtaaaaatc gagtatgtgc tagatgctat ggaagataca aattcagcgg
     1981 tcatcactgt ataaattgca agtatgtacc agaagcacgt gaagtgaaaa aggcaaaaga
     2041 caaaggcgaa aaattgggca ttacgcccga aggtttgcca gttaaaggac cagagtgtat
     2101 aaaatgtggc ggaatcttac agtttgatgc tgtccgcggg cctcataaga gttgtggtaa
     2161 caacgcaggt gcgcgcatct gcgatatcga ctacaaggat gacgacgaca agaagcttta
     2221 actaactaac ctcgagacag gccccttttc ctttgtctat atcatgtaat tagttatgtc
     2281 acgcttacat tcacgccctc ctcccacatc cgctctaacc gaaaaggaag gagttagaca
     2341 acctgaagtc taggtcccta tttatttttt ttaatagtta tgttagtatt aagaacgtta
     2401 tttatatttc aaatttttct tttttttctg tacaaacgcg tgtacgcatg taacattata
     2461 ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg caaggttcgc
     2521 agtttacact ctcatcgtcg ctctcatcat cgcttccgtt gttgttttcc ttagtagcgt
     2581 ctgcttggta cccagcttcg gccgctctag agaagcgggt aagctgccac agcaattaat
     2641 gcacaacatt taacctacat tcttccttat cggatcctca aaacccttaa aaacatatgc
     2701 ctcaccctaa catattttcc aattaaccct caatatttct ctgtcacccg gcctctattt
     2761 tccattttct tctttacccg ccacgcgttt ttttctttca aatttttttc ttccttcttc
     2821 tttttcttcc acgtcctctt gcataaataa ataaaccgtt ttgaaaccaa actcgcctct
     2881 ctctctcctt tttgaaatat ttttgggttt gtttgatcct ttccttccca atctctcttg
     2941 tttaatatat attcatttat atcacgctct ctttttatct tccttttttt cctctctctt
     3001 gtattcttcc ttcccctttc tactcaaacc aagaagaaaa agaaaaggtc aatctttgtt
     3061 aaagaatagg atcttctact acatcagctt ttagattttt cacgcttact gcttttttct
     3121 tcccaagatc gaaaatttac tgaattaaca actagtggat cccccgggct gcaggaattc
     3181 atgaaagcgt taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc
     3241 cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca
     3301 aacgcggctg aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc
     3361 ggcgcatcac gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt
     3421 cgtgtggctg ccggtgaacc acttctggcg caacagcata ttgaaggtca ttatcaggtc
     3481 gatccttcct tattcaagcc gaatgctgat ttcctgctgc gcgtcagcgg gatgtcgatg
     3541 aaagacatcg gcattatgga tggtgacttg ctggcagtgc ataaaactca ggatgtacgt
     3601 aacggtcagg tcgttgtcgc acgtattgat gacgaggtta ccgttaagcg cctgaaaaaa
     3661 cagggcaata aagtcgaact gttgccagaa aatagcgagt ttaaaccaat tgtcgtagat
     3721 cttcgtcagc agagcttcac cattgaaggg ctggcggttg gggttattcg caacggcgac
     3781 tggctgggtt ctaaggatat ctctgctgga gacatgagag ctgccaacct ttggccaagc
     3841 ccgctcatga tcaaacgctc taagaagaac agcctggcct tgtccctgac ggccgaccag
     3901 atggtcagtg ccttgttgga tgctgagccc cccatactct attccgagta tgatcctacc
     3961 agacccttca gtgaagcttc gatgatgggc ttactgacca acctggcaga cagggagctg
     4021 gttcacatga tcaactgggc gaagagggtg ccaggctttg tggatttgac cctccatgat
     4081 caggtccacc ttctagaatg tgcctggtta gagatcctga tgattggtct cgtctggcgc
     4141 tccatggagc acccagtgaa gctactgttt gctcctaact tgctcttgga caggaaccag
     4201 ggaaaatgtg tagagggcat ggtggagatc ttcgacatgc tgctggctac atcatctcgg
     4261 ttccgcatga tgaatctgca gggagaggag tttgtgtgcc tcaaatctat tattttgctt
     4321 aattctggag tgtacacatt tctgtccagc accctgaagt ctctggaaga gaaggaccat
     4381 atccaccgag tcctggacaa gatcacagac actttgatcc acctgatggc caaggcaggc
     4441 ctgaccctgc agcagcagca ccagcggctg gcccagctcc tcctcatcct ctcccacatc
     4501 aggcacatga gtaacaaagg catggagcat ctgtacagca tgaagtgcaa gaacgtggtg
     4561 cccctctatg acctgctgct ggagatgctg gacgcccacc gcctacatgc gcccactagc
     4621 cgtggagggg catccgtgga ggagacggac caaagccact tggccactgc gggctctact
     4681 tcatcgcatt ccttgcaaaa gtattacatc acgggggagg cagagggttt ccctgccaca
     4741 gtcgatatca tgcaggatct gccgggcaac gataacagca ccgcgggcga atttccaggt
     4801 attactttga gaatccaaga aactgatatg ttgtataaag gtgatacttt gtatttggat
     4861 tggttggaag atggtattgc tgaattggtt ttcgatgctc caggttctgt taataaattg
     4921 gatactgctg ttgcttcttt gggtgaagct attggtgttt tggaacaaca atctgatttg
     4981 atttgggaaa ctttgactgt taaagatgct aaagttaatt tcgattctgg tttggaaaaa
     5041 ttcgaagaag ctattccatc tgctgatgat ttcgatccag ttgctgaaag aagatcctct
     5101 ggtgaattta gagctgaaag acattctggt ggtactgatt tgtgtttcaa gctttaaacg
     5161 cgtttaaagc ttatcctcga gacaggcccc ttttcctttg tctatatcat gtaattagtt
     5221 atgtcacgct tacattcacg ccctcctccc acatccgctc taaccgaaaa ggaaggagtt
     5281 agacaacctg aagtctaggt ccctatttat tttttttaat agttatgtta gtattaagaa
     5341 cgttatttat atttcaaatt tttctttttt ttctgtacaa acgcgtgtac gcatgtaaca
     5401 ttatactgaa aaccttgctt gagaaggttt tgggacgctc gaaggcttta atttgcaagg
     5461 ttcgcagttt acactctcat cgtcgctctc atcatcgctt ccgttgttgt tttccttagt
     5521 agcgtctgct tggtacccag cttcggccgt atttacagat aacgatgtcg ttattatata
     5581 tatatatata tattgtcact tgtacccagc ttttgttccc tttagtgagg gttaatttcg
     5641 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaaca
     5701 gctgacaccg attatttaaa gctgcagcat acgatatata tacatgtgta tatatgtata
     5761 cctatgaatg tcagtaagta tgtatacgaa cagtatgata ctgaagatga caaggtaatg
     5821 catcattcta tacgtgtcat tctgaacgag gcgcgcctct ccctgcaggc attaatgaat
     5881 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5941 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6001 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6061 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6121 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6181 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     6241 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     6301 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     6361 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6421 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6481 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6541 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6601 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6661 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt gtttaaacct
     6721 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6781 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6841 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6901 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6961 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     7021 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     7081 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     7141 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     7201 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     7261 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatggttgtc
     7321 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     7381 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     7441 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     7501 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     7561 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     7621 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     7681 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7741 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7801 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7861 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
     7921 ttaattaact ctggcgcgcc tcctctagta cactctatat ttttttatgc ctcggtaatg
     7981 attttcattt ttttttttcc acctagcgga tgactctttt tttttcttag cgattggcat
     8041 tatcacataa tgaattatac attatataaa gtaatgtgat ttcttcgaag aatatactaa
     8101 aaaatgagca ggcaagataa acgaaggcac atatgaggag ggcttttgta gaaagaaata
     8161 cgaacgaaac gaaaatcagc gttgccatcg ctttggacaa agctccctta cctgaagagt
     8221 cgaattttat tgatgaactt ataacttcca agcatgcaaa ccaaaaggga gaacaagtaa
     8281 tccaagtaga cacgggaatt ggattcttgg atcacatgta tcatgcactg gctaaacatg
     8341 caggctggag cttacgactt tactcaagag gtgatttaat catcgatgat catcacactg
     8401 cagaagatac tgctattgca cttggtattg cattcaagca ggctatgggt aactttgccg
     8461 gtgttaaaag atttggacat gcttattgtc cacttgacga agctctttcc agaagcgtag
     8521 ttgacttgtc gggacggccc tatgctgtta tcgatttggg attaaagcgt gaaaaggttg
     8581 gggaattgtc ctgtgaaatg atccctcact tactatattc cttttcggta gctgctggaa
     8641 ttactttgca tgttacctgc ttatatggta gtaatgacca tcatcgtgct gaaagcgctt
     8701 ttaaatctct ggctgttgcc atgcgcgcgg ctacttctct tactggaagt tctgaagtcc
     8761 caagcacgaa gggagtgttg taaagatgaa ttggattatg tcaggaaaag aacgacaatt
     8821 ttgcatccaa attgtctaaa ttttagagtt gcttgaaaac aatagaacct tacttgcttt
     8881 ataattacgt taattagaag cgttatctcg tgaaggaata tagtacgtag ccgtataaat
     8941 tgaattgaat gttcagctta tagaatagag acactttgct gttcaatgcg tcgtcactta
     9001 ccatactcac tttattatac gactttaagt ataaactccg cggttatggt aaaattaatg
     9061 atgcacaaac gtccgatcgg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     9121 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc a
//
