LOCUS       sequence-309483-        9619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For protein expression of BhCas12b_v4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9619)
  AUTHORS   Strecker J, Jones S, Koopal B, Schmid-Burgk J, Zetsche B, Gao L, 
            Makarova KS, Koonin EV, Zhang F
  TITLE     Engineering of CRISPR-Cas12b for human genome editing.
  JOURNAL   Nat Commun. 2019 Jan 22;10(1):212. doi: 10.1038/s41467-018-08224-4.
  PUBMED    30670702
REFERENCE   2  (bases 1 to 9619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-22"; volume: "10(1):212. doi"; pages: " 
            10.1038/s41467-018-08224-4"
COMMENT     Sequence Label: TwinStrep-SUMO-NLS-BhCas12b_v4
FEATURES             Location/Qualifiers
     source          1..9619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     163..182
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     266..285
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        266..284
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    285..309
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             324..346
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             365..382
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             392..409
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             join(422..445,446..481,482..505)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="WSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     CDS             506..799
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     CDS             809..829
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4175..4222
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             4229..4309
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(4385..4403)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4399..4446
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(4621..4626,4627..4643,4644..4649))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(4676..4694)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4762..4866
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4867..4935,4936..5727)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5085..5104)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5898..6486
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6387..6406
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6640..6657
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6672..6811
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6737..6756)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6897..6919
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6913..7104)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8416..9498)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9460..9479
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9499..9576)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
       61 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      121 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      181 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      241 gatcgagatc tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat
      301 aacaattccc ctctagaaat aattttgttt aactttaaga aggagatata ccatgggcag
      361 cagccatcat catcatcatc acagcagcgg cctggtgccg cgcggcagcc atatggctag
      421 ctggagccat ccgcagtttg aaaaaggtgg tggtagcggt ggtggttcag gtggtagtgc
      481 atggtcacac cctcagtttg agaaaatgtc ggactcagaa gtcaatcaag aagctaagcc
      541 agaggtcaag ccagaagtca agcctgagac tcacatcaat ttaaaggtgt ccgatggatc
      601 ttcagagatc ttcttcaaga tcaaaaagac cactccttta agaaggctga tggaagcgtt
      661 cgctaaaaga cagggtaagg aaatggactc cttaagattc ttgtacgacg gtattagaat
      721 ccaagctgat cagacccctg aagatttgga catggaggat aacgatatta ttgaggctca
      781 cagagaacag attggtggat ccatggcccc aaagaagaag cggaaggtcg gtatccacgg
      841 agtcccagca gccgccacca gatccttcat cctgaagatc gagcccaacg aggaagtgaa
      901 gaaaggcctc tggaaaaccc acgaggtgct gaaccacgga atcgcctact acatgaatat
      961 cctgaagctg atccggcaag aggccatcta cgagcaccac gagcaggacc ccaagaatcc
     1021 caagaaggtg tccaaggccg agatccaggc cgagctgtgg gatttcgtgc tgaagatgca
     1081 gaagtgcaac agcttcacac acgaggtgga caaggacgag gtgttcaaca tcctgagaga
     1141 gctgtacgag gaactggtgc ccagcagcgt ggaaaagaag ggcgaagcca accagctgag
     1201 caacaagttt ctgtaccctc tggtggaccc caacagccag tctggaaagg gaacagccag
     1261 cagcggcaga aagcccagat ggtacaacct gaagattgcc ggcgatccct cctgggaaga
     1321 agagaagaag aagtgggaag aagataagaa aaaggacccg ctggccaaga tcctgggcaa
     1381 gctggctgag tacggactga tccctctgtt catcccctac accgacagca acgagcccat
     1441 cgtgaaagaa atcaagtgga tggaaaagtc ccggaaccag agcgtgcggc ggctggataa
     1501 ggacatgttc attcaggccc tggaacggtt cctgagctgg gagagctgga acctgaaagt
     1561 gaaagaggaa tacgagaagg tcgagaaaga gtacaagacc ctggaagaga ggatcaaaga
     1621 ggacatccag gctctgaagg ctctggaaca gtatgagaaa gagcggcaag aacagctgct
     1681 gcgggacacc ctgaacacca acgagtaccg gctgagcaag agaggcctta gaggctggcg
     1741 ggaaatcatc cagaaatggc tgaaaatgga cgagaacgag ccctccgaga agtacctgga
     1801 agtgttcaag gactaccagc ggaagcaccc tagagaggcc ggcgattaca gcgtgtacga
     1861 gttcctgtcc aagaaagaga accacttcat ctggcggaat caccctgagt acccctacct
     1921 gtacgccacc ttctgcgaga tcgacaagaa aaagaaggac gccaagcagc aggccacctt
     1981 cacactggcc gatcctatca atcaccctct gtgggtccga ttcgaggaaa gaagcggcag
     2041 caacctgaac aagtacagaa tcctgaccga gcagctgcac accgagaagc tgaagaaaaa
     2101 gctgacagtg cagctggacc ggctgatcta ccctacagaa tctggcggct gggaagagaa
     2161 gggcaaagtg gacattgtgc tgctgcccag ccggcagttc tacaaccaga tcttcctgga
     2221 catcgaggaa aagggcaagc acgccttcac ctacaaggat gagagcatca agttccctct
     2281 gaagggcaca ctcggcggag ccagagtgca gttcgacaga gatcacctga gaagataccc
     2341 tcacaaggtg gaaagcggca acgtgggcag aatctacttc aacatgaccg tgaacatcga
     2401 gcctacagag tccccagtgt ccaagtctct gaagatccac cgggacgact tccccaaggt
     2461 ggtcaacttc aagcccaaag aactgaccga gtggatcaag gacagcaagg gcaagaaact
     2521 gaagtccggc atcgagtccc tggaaatcgg cctgagagtg atgagcatcg acctgggaca
     2581 gagacaggcc gctgccgcct ctattttcga ggtggtggat cagaagcccg acatcgaagg
     2641 caagctgttt ttcccaatca agggcaccga gctgtatgcc gtgcacagag ccagcttcaa
     2701 catcaagctg cccggcgaga cactggtcaa gagcagagaa gtgctgcgga aggccagaga
     2761 ggacaatctg aaactgatga accagaagct caacttcctg cggaacgtgc tgcacttcca
     2821 gcagttcgag gacatcaccg agagagagaa gcgggtcacc aagtggatca gcagacaaga
     2881 gaacagcgac gtgcccctgg tgtaccagga tgagctgatc cagatccgcg agctgatgta
     2941 caagccttac aaggactggg tcgccttcct gaagcagctc cacaagagac tggaagtcga
     3001 gatcggcaaa gaagtgaagc actggcggaa gtccctgagc gacggaagaa agggcctgta
     3061 cggcatctcc ctgaagaaca tcgacgagat cgatcggacc cggaagttcc tgctgagatg
     3121 gtccctgagg cctaccgaac ctggcgaagt gcgtagactg gaacccggcc agagattcgc
     3181 catcgaccag ctgaatcacc tgaacgccct gaaagaagat cggctgaaga agatggccaa
     3241 caccatcatc atgcacgccc tgggctactg ctacgacgtg cggaagaaga aatggcaggc
     3301 taagaacccc gcctgccaga tcatcctgtt cgaggatctg agcaactaca acccctacgg
     3361 agaaaggtcc cgcttcgaga acagccggct catgaagtgg tccagacgcg agatccccag
     3421 acaggttgca ctgcagggcg agatctatgg cctgcaagtg ggagaagtgg gcgctcagtt
     3481 cagcagcaga ttccacgcca agacaggcag ccctggcatc agatgtcggg tcgtgaccaa
     3541 agagaagctg caggacaatc ggttcttcaa gaatctgcag agagagggca gactgaccct
     3601 ggacaaaatc gccgtgctga aagagggcga tctgtaccca gacaaaggcg gcgagaagtt
     3661 catcagcctg agcaaggatc ggaagtgcgt gaccacacac gccgacatca acgccgctca
     3721 gaacctgcag aagcggttct ggacaagaac ccacggcttc tacaaggtgt actgcaaggc
     3781 ctaccaggtg gacggccaga ccgtgtacat ccctgagagc aaggaccaga agcagaagat
     3841 catcgaagag ttcggcgagg gctacttcat tctgaaggac ggggtgtacg aatgggtcaa
     3901 cgccggcaag ctgaaaatca agaagggcag ctccaagcag agcagcagcg agctggtgga
     3961 tagcgacatc ctgaaagaca gcttcgacct ggcctccgag ctgaaaggcg aaaagctgat
     4021 gctgtacagg gaccccagcg gcaatgtgtt ccccagcgac aaatggatgg ccgctggcgt
     4081 gttcttcgga aagctggaac gcatcctgat cagcaagctg accaaccagt actccatcag
     4141 caccatcgag gacgacagca gcaagcagtc tatgaaaagg ccggcggcca cgaaaaaggc
     4201 cggccaggca aaaaagaaaa agggatccta cccatacgat gttccagatt acgcttatcc
     4261 ctacgacgtg cctgattatg catacccata tgatgtcccc gactatgcct aagaattctg
     4321 cagatatcca gcacagtggc ggccgcactc gaggcccgaa aggaagctga gttggctgct
     4381 gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt
     4441 tttttgctga aaggaggaac tatatccgga tatcccgcaa gaggcccggc agtaccggca
     4501 taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat
     4561 tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc
     4621 attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc
     4681 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt
     4741 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     4801 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     4861 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     4921 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     4981 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     5041 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     5101 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     5161 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     5221 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     5281 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     5341 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     5401 acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     5461 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     5521 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     5581 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     5641 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     5701 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     5761 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     5821 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     5881 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     5941 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     6001 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     6061 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     6121 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     6181 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     6241 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     6301 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     6361 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     6421 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     6481 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     6541 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     6601 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     6661 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     6721 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     6781 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     6841 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     6901 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     6961 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     7021 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     7081 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     7141 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     7201 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     7261 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
     7321 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
     7381 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt
     7441 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
     7501 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
     7561 gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat
     7621 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa
     7681 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc
     7741 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg
     7801 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa
     7861 atcgccgtga cgatcagcgg tccaatgatc gaagttaggc tggtaagagc cgcgagcgat
     7921 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg
     7981 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc
     8041 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc
     8101 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc
     8161 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc
     8221 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag
     8281 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg
     8341 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     8401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8461 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     8521 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     8581 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     8641 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc
     8701 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     8761 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     8821 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     8881 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     8941 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     9001 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     9061 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     9121 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     9181 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     9241 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     9301 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     9361 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     9421 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     9481 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     9541 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
     9601 gcgactcctg cattaggaa
//
