LOCUS       sequence-30956-e         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Tanida I, Yamaji T, Ueno T, Ishiura S, Kominami E, Hanada K
  TITLE     Consideration about negative controls for LC3 and expression vectors
            for four colored fluorescent protein-LC3 negative controls.
  JOURNAL   Autophagy. 2008 Jan 1. 4(1):131-4.
  PUBMED    18000393
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Autophagy. 
            2008 Jan 1. 4(1):131-4."
COMMENT     Sequence Label: pEX-GFP-hLC3WT
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..443)
                     /codon_start=1
                     /gene="MAP1LC3B"
                     /product="human LC3 protein"
                     /label=LC3B
                     /note="Atg8 family member found on autophagosomes"
                     /translation="MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLP
                     VLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKD
                     EDGFLYMVYASQETFGMKLSV"
     primer_bind     complement(501..522)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 caccagccac caccttctga taggcagcct gcacctgagg agtgaattga gctcccgggc
       61 tcgacttaca ctgacaattt catcccgaac gtctcctggg aggcgtagac catgtacagg
      121 aatccatctt catctttctc actctcatac acctctgaga ttggtgtgga gacgctgacc
      181 atgctgtgtc cgttcaccaa caggaagaag gcctgattag cattgagctg taagcgcctt
      241 ctaattatct tgatgagctc actcatgttg acatggtcag gtacaaggaa ctttgtttta
      301 tccagaacag gaagctgctt ctcacccttg tatcgttcta ttatcaccgg gattttggtt
      361 ggatgctgct ctcgaataag tcggacatct tctactcttt gttcgaaggt gcggcgctgc
      421 ttgaaggtct tctccgacgg catagatctg agtccggact tgtacagctc gtccatgccg
      481 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      541 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
      601 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
      661 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
      721 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccanggatgt tgccgtcctc
      781 cttgaagtcg atgccccttc agctcgatgc gggttcacca gggtgtc
//
