LOCUS       sequence-309701-       10513 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The human DPYD was used to study the effect of DPYD expression on 
            EMT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Shaul YD, Freinkman E, Comb WC, Cantor JR, Tam WL, Thiru P, Kim D, 
            Kanarek N, Pacold ME, Chen WW, Bierie B, Possemato R, Reinhardt F, 
            Weinberg RA, Yaffe MB, Sabatini DM
  TITLE     Dihydropyrimidine accumulation is required for the 
            epithelial-mesenchymal transition.
  JOURNAL   Cell. 2014 Aug 28;158(5):1094-109. doi: 10.1016/j.cell.2014.07.032.
  PUBMED    25171410
REFERENCE   2  (bases 1 to 10513)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.07"; journalName: "Cell"; date: "2014-08-28- 
            28"; volume: "158"; issue: "5"; pages: "1094-109"
COMMENT     Sequence Label: DPYD-FLAG
FEATURES             Location/Qualifiers
     source          1..10513
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     primer_bind     2014..2034
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             5675..5740
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5788..5905
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5954..6464
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     6383..6401
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             6486..7085
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6486..6505)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6982..7002
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7213..7446
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7518..7639
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7555..7574)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7609..7628
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7679..7814
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7834..7853)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7835..7853)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7863..7880)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7863..7879)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7872..7894)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8021..8476
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8108..8127)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8318..8339
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8502..8606
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8607..8675,8676..9467)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8825..8844)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9638..10226
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10127..10146
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10380..10397
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagca gcagctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgatca
     1981 cgagactagc ctcgagcagc agcccccttc accgagggcc tatttcccat gattccttca
     2041 tatttgcata tacgatacaa ggctgttaga gagataattg gaattaattt gactgtaaac
     2101 acaaagatat tagtacaaaa tacgtgacgt agaaagtaat aatttcttgg gtagtttgca
     2161 gttttaaaat tatgttttaa aatggactat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtttaatta acactgccat
     2581 ggcccctgtg ctcagtaagg actcggcgga catcgagagt atcctggctt taaatcctcg
     2641 aacacaaact catgcaactc tgtgttccac ttcggccaag aaattagaca agaaacattg
     2701 gaaaagaaat cctgataaga actgctttaa ttgtgagaag ctggagaata attttgatga
     2761 catcaagcac acgactcttg gtgagcgagg agctctccga gaagcaatga gatgcctgaa
     2821 atgtgcagat gccccgtgtc agaagagctg tccaactaat cttgatatta aatcattcat
     2881 cacaagtatt gcaaacaaga actattatgg agctgctaag atgatatttt ctgacaaccc
     2941 acttggtctg acttgtggaa tggtatgtcc aacctctgat ctttgtgtag gtggatgcaa
     3001 tttatatgcc actgaagagg gacccattaa tattggtgga ttgcagcaat ttgctactga
     3061 ggtattcaaa gcaatgagta tcccacagat cagaaatcct tcgctgcctc ccccagaaaa
     3121 aatgtctgaa gcctattctg caaagattgc tctttttggt gctgggcctg caagtataag
     3181 ttgtgcttcc tttttggctc gattggggta ctctgacatc actatatttg aaaaacaaga
     3241 atatgttggt ggtttaagta cttctgaaat tcctcagttc cggctgccgt atgatgtagt
     3301 gaattttgag attgagctaa tgaaggacct tggtgtaaag ataatttgcg gtaaaagcct
     3361 ttcagtgaat gaaatgactc ttagcacttt gaaagaaaaa ggctacaaag ctgctttcat
     3421 tggaataggt ttgccagaac ccaataaaga tgccatcttc caaggcctga cgcaggacca
     3481 ggggttttat acatccaaag actttttgcc acttgtagcc aaaggcagta aagcaggaat
     3541 gtgcgcctgt cactctccat tgccatcgat acggggagtc gtgattgtac ttggagctgg
     3601 agacactgcc tttgactgtg caacatctgc tctacgttgt ggagctcgcc gtgtgttcat
     3661 cgtcttcaga aaaggctttg ttaatataag agctgtccct gaggagatgg aacttgctaa
     3721 ggaagaaaag tgtgaatttc tgccattcct gtccccacgg aaggttatag taaaaggtgg
     3781 gagaattgtt gctatgcagt ttgttcggac agagcaagat gaaactggaa aatggaatga
     3841 agatgaagat cagatggtcc atctgaaagc cgatgtggtc atcagtgcct ttggttcagt
     3901 tctgagtgat cctaaagtaa aagaagcctt gagccctata aaatttaaca gatggggtct
     3961 cccagaagta gatccagaaa ctatgcaaac tagtgaagca tgggtatttg caggtggtga
     4021 tgtcgttggt ttggctaaca ctacagtgga atcggtgaat gatggaaagc aagcttcttg
     4081 gtacattcac aaatacgtac agtcacaata tggagcttcc gtttctgcca agcctgaact
     4141 acccctcttt tacactccta ttgatctggt ggacattagt gtagaaatgg ccggattgaa
     4201 gtttataaat ccttttggtc ttgctagcgc aactccagcc accagcacat caatgattcg
     4261 aagagctttt gaagctggat ggggttttgc cctcaccaaa actttctctc ttgataagga
     4321 cattgtgaca aatgtttccc ccagaatcat ccggggaacc acctctggcc ccatgtatgg
     4381 ccctggacaa agctcctttc tgaatattga gctcatcagt gagaaaacgg ctgcatattg
     4441 gtgtcaaagt gtcactgaac taaaggctga ctttccagac aacattgtga ttgctagcat
     4501 tatgtgcagt tacaataaaa atgactggac ggaacttgcc aagaagtctg aggattctgg
     4561 agcagatgcc ctggagttaa atttatcatg tccacatggc atgggagaaa gaggaatggg
     4621 cctggcctgt gggcaggatc cagagctggt gcggaacatc tgccgctggg ttaggcaagc
     4681 tgttcagatt cctttttttg ccaagctgac cccaaatgtc actgatattg tgagcatcgc
     4741 aagagctgca aaggaaggtg gtgccaatgg cgttacagcc accaacactg tctcaggtct
     4801 gatgggatta aaatctgatg gcacaccttg gccagcagtg gggattgcaa agcgaactac
     4861 atatggagga gtgtctggga cagcaatcag acctattgct ttgagagctg tgacctccat
     4921 tgctcgtgct ctgcctggat ttcccatttt ggctactggt ggaattgact ctgctgaaag
     4981 tggtcttcag tttctccata gtggtgcttc cgtcctccag gtatgcagtg ccattcagaa
     5041 tcaggatttc actgtgatcg aagactactg cactggcctc aaagccctgc tttatctgaa
     5101 aagcattgaa gaactacaag actgggatgg acagagtcca gctactgtga gtcaccagaa
     5161 agggaaacca gttccacgta tagctgaact catggacaag aaactgccaa gttttggacc
     5221 ttatctggaa cagcgcaaga aaatcatagc agaaaacaag attagactga aagaacaaaa
     5281 tgtagctttt tcaccactta agagaaactg ttttatcccc aaaaggccta ttcctaccat
     5341 caaggatgta ataggaaaag cactgcagta ccttggaaca tttggtgaat tgagcaacgt
     5401 agagcaagtt gtggctatga ttgatgaaga aatgtgtatc aactgtggta aatgctacat
     5461 gacctgtaat gattctggct accaggctat acagtttgat ccagaaaccc acctgcccac
     5521 cataaccgac acttgtacag gctgtactct gtgtctcagt gtttgcccta ttgtcgactg
     5581 catcaaaatg gtttccagga caacacctta tgaaccaaag agaggcgtac ccttatctgt
     5641 gaatccggtg tgtggaggca gcgcggccgc aggcgactac aaagaccatg acggtgatta
     5701 taaagatcat gacattgatt acaaggatga cgatgacaag taataagaat tctcgacctc
     5761 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     5821 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     5881 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
     5941 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
     6001 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
     6061 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
     6121 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
     6181 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
     6241 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
     6301 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
     6361 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
     6421 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggagc
     6481 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     6541 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     6601 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     6661 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     6721 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     6781 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     6841 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     6901 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     6961 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     7021 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     7081 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     7141 cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
     7201 aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt
     7261 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     7321 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     7381 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     7441 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
     7501 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     7561 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     7621 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
     7681 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7741 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     7801 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
     7861 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     7921 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     7981 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     8041 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     8101 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     8161 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     8221 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     8281 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     8341 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     8401 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     8461 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     8521 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     8581 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     8641 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     8701 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     8761 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     8821 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     8881 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     8941 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     9001 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     9061 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     9121 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     9181 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     9241 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     9301 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     9361 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     9421 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     9481 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     9541 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     9601 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     9661 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     9721 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     9781 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     9841 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     9901 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     9961 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    10021 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    10081 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    10141 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    10201 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    10261 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    10321 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    10381 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    10441 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    10501 agcgcaacgc aat
//
