LOCUS       sequence-309724-        7031 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation plasmid for LetAB with LetB PLL3 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
  TITLE     LetB Structure Reveals a Tunnel for Lipid Transport across the 
            Bacterial Envelope.
  JOURNAL   Cell. 2020 Apr 30;181(3):653-664.e19. doi: 
            10.1016/j.cell.2020.03.030.
  PUBMED    32359438
REFERENCE   2  (bases 1 to 7031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.03"; journalName: "Cell"; date: "2020-04-30- 
            30"; volume: "181"; issue: "3"; pages: "653-664.e19"
COMMENT     Sequence Label: pBEL1888
FEATURES             Location/Qualifiers
     source          1..7031
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        100..118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             150..172
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(4088..4106)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4102..4149
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4194..4212)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4280..4384
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4385..4453,4454..5245)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4603..4622)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5416..6004
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5905..5924
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6158..6175
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6190..6329
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6255..6274)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6415..6437
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6431..6622)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
       61 caggacccaa cgctgcccga gatctcgatc ccgcgaaatt aatacgactc actataggga
      121 gaccacaacg gtttccctct agaaataatt ttgtttaact ttaagaagga gatatacata
      181 tggctcttaa cacaccacaa attacgccga caaaaaagat aacagtgagg gcaatcggcg
      241 aggaactgcc gcgtggtgat taccaacgtt gcccgcaatg tgacatgctg tttagcctgc
      301 ccgagataaa ttctcatcaa agtgcctatt gtccgcgctg tcaggcaaaa attcgtgacg
      361 ggcgcgactg gtcgctaacg cgcctggcgg caatggcctt caccatgctg ttgcttatgc
      421 cgtttgcctg gggcgaaccg ctgttgcata tctggctgtt aggcattcgt attgacgcca
      481 acgttatgca aggcatctgg caaatgacca aacagggcga tgcgataacg gggtcgatgg
      541 tctttttctg cgttattggt gccccgctca ttctggtgac ctccatagct tatttatggt
      601 ttggtaaccg actgggaatg aatttacgtc cggtactgct gatgcttgag cgacttaaag
      661 agtgggtaat gctcgatatc tacctggtcg gcattggcgt tgcttctata aaggtacagg
      721 attatgccca tatccaggct ggtgtcggct tgttctcttt tgtggcgttg gtgattttaa
      781 cgacggtgac gttgtcacat cttaatgtcg aagaactgtg ggagcgattt tatccgcagc
      841 gccccgctac gcgtagggac gagaaacttc gtgtctgcct agggtgccat tttaccggct
      901 atccagatca gcgtggtcgc tgcccgcgtt gccatatccc gctacgcctg cgtcgccgtc
      961 atagtctgca aaaatgctgg gcggcgctgt tagcgtcaat cgttttattg ttacctgcca
     1021 acctgttgcc tatttctatc atttatctga acggaggacg gcaggaagat acaattcttt
     1081 ccggaattat gtcgctggca agtagcaaca ttgcggttgc aggaatcgtg tttatcgcca
     1141 gtattctggt cccgtttact aaagtgatcg tcatgttcac tttactgttg agcattcatt
     1201 ttaaatgcca gcaaggttta cgcacacgca ttctgttact gcggatggtg acctggattg
     1261 gtcgctggtc gatgctcgac ctgtttgtca tatctttaac catgtcgctg attaatcgcg
     1321 atcagatcct cgcttttact atgggaccgg ctgcgtttta tttcggcgca gcggtaattt
     1381 tgactattct tgctgtggaa tggctggaca gccgcttact ttgggatgca catgagtcag
     1441 gaaacgcccg cttcgacgac tgaagcgcag attaaaaata aacgccgtat ctcacctttc
     1501 tggctgctgc cgttcatcgc gctaatgatt gccagttggc tgatttggga cagttatcag
     1561 gaccggggta ataccgtcac catcgacttt atgtcggcgg atggtattgt tccaggccgt
     1621 acgcctgttc gttatcaggg cgttgaagtc ggaacagtgc aggacatcag cctcagcgac
     1681 gatcttcgta agattgaagt caaggtcagc atcaagtccg atatgaaaga tgcgctgcgc
     1741 gaagagactc agttctggct ggtgacgcca aaagcatcgt tggcaggtgt ctccgggctg
     1801 gacgccctcg tcggtgggaa ctatatcggc atgatgcccg ggaaaggtaa agagcaggat
     1861 cactttgtcg cactcgatac ccaaccgaaa tatcggctgg acaatggcga tctgatgatc
     1921 cacctgcaag cccccgatct cggttcgctg aacagcggtt cattggtcta tttccgcaag
     1981 atcccggtgg gaaaagtcta cgactatgcc atcaatccca acaagcaagg cgtggtgatt
     2041 gatgtcctga tcgagcggcg ttttaccgat ctggtgaaaa aaggtagccg tttctggaac
     2101 gtttccggcg ttgatgccaa cgtcagtatc agtggcgcga aggtgaaact ggaaagcttg
     2161 gcggcactgg ttaacggtgc gattgccttc gattcaccag aagagtcgaa acctgccgag
     2221 gcggaagata cctttggtct gtatgaagat ctggcccaca gccagcgtgg cgtaataata
     2281 aaactggaac tgccgagtgg ggccggatta accgccgact cgacgccgtt aatgtatcag
     2341 gggctggaag tcggacagct gactaaactg gatttaaatc ctggtggtaa agtcacaggg
     2401 gaaatgaccg ttgatcccag cgtcgttacc ctgcttcgtg aaaatacccg catcgaatta
     2461 cgcaacgggg gcaaaacctt cgagctggtg cccggcgatg gcgagccacg caaagaattc
     2521 gttgttgtgc caggcgaaaa agcactgctg catgaacctg atgttctgac gctgaccctg
     2581 accgcgccgg aaagttacgg tattgatgcg ggtcagccgc tcattcttca cggcgtgcag
     2641 gtaggccagg ttatcgatcg taaactcacc agcaaaggcg tcacctttac cgtcgccatc
     2701 gagcctcagc atcgcgaact ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc
     2761 gatgtgaagg tggggctgga tggcgttgag tttctcggtg ccagcgcctc agaatggatc
     2821 aatggcggga tccgtattct gccgggcgat aaaggtgaga tgaaagccag ctatccactg
     2881 tatgccaatt tggaaaaagc gctggagaac agccttagcg atttacccac cacaaccgtg
     2941 agtttgagtg cagagacgct gccggatgtg caggcaggat cggtagtgct gtaccgtaaa
     3001 tttgaagttg gtgaagtgat taccgtgcgt ccgcgagcta acgcgtttga catcgatctg
     3061 catattaagc cggagtatcg caaccttctg accagcaata gcgtgttctg ggcagaaggc
     3121 ggggcgaaag ttcagctgaa tggtagtggt ctgaccgtac aggcatcccc gctctccaga
     3181 gctctaaagg gagccattag cttcgacaac ctcagcggtg ccagcgccag tcagcgtaaa
     3241 ggcgataagc gtattctgta tgcttccgaa acagcggccc gtgcggttgg cgggcagatt
     3301 acgcttcacg ctttcgatgc cggaaaactg gcggtcggga tgccaattcg ctatctcggt
     3361 attgacatcg ggcaaatcca gacgctggat ctgattaccg cacgcaatga agtacaggca
     3421 aaggcggtac tttacccgga atatgtccag acctttgctc gcggtggtac gcgcttctca
     3481 gtggtcacac cgcaaatttc ggcagctggc gttgagcatc ttgatactat cctccagccg
     3541 tatatcaacg tcgaaccagg ccggggcaat cctcgccgcg actttgaatt acaagaggcc
     3601 accattactg attcgcggta cctggatggc ttaagcatta ttgttgaagc gccggaagcc
     3661 ggttcgttag gcatcggtac gcctgtgctg ttccgtggtc tggaagtcgg tacggttaca
     3721 ggaatgacgc tggggacatt gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc
     3781 tatcaacacc tggtgcgtaa caattccgtc ttctggttgg catcgggtta cagtctggac
     3841 tttggtctga cgggcggcgt agtgaaaacc ggcaccttta accagtttat ccgtggcggc
     3901 atcgccttcg ccacgcctcc gggtacgcca ctggcaccga aagcccagga aggcaaacac
     3961 ttcctgttgc aggaaagtga accgaaagag tggcgtgaat ggggaactgc gcttcccaaa
     4021 taatgcccac ctcgagcaga tccggctgct aacaaagccc gaaaggaagc tgagttggct
     4081 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg
     4141 ggttttttgc tgaaaggagg aactatatcc ggataattct tgaagacgaa agggcctcgt
     4201 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4261 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4321 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4381 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     4441 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     4501 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     4561 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4621 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4681 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4741 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4801 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4861 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4921 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4981 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5041 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5101 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5161 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5221 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5281 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5341 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     5401 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     5461 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     5521 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     5581 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5641 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5701 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5761 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5821 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5881 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5941 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6001 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6061 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6121 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6181 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa
     6241 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     6301 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     6361 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     6421 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa
     6481 gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct
     6541 cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg
     6601 cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg
     6661 taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg
     6721 cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga
     6781 gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg
     6841 gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc
     6901 gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg
     6961 cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct
     7021 aaccagtaag g
//
