LOCUS       sequence-309736-        7040 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Complementation plasmid for LetAB with LetB PLL7 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7040)
  AUTHORS   Isom GL, Coudray N, MacRae MR, McManus CT, Ekiert DC, Bhabha G
  TITLE     LetB Structure Reveals a Tunnel for Lipid Transport across the 
            Bacterial Envelope.
  JOURNAL   Cell. 2020 Apr 30;181(3):653-664.e19. doi: 
            10.1016/j.cell.2020.03.030.
  PUBMED    32359438
REFERENCE   2  (bases 1 to 7040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2020.03"; journalName: "Cell"; date: "2020-04-30- 
            30"; volume: "181"; issue: "3"; pages: "653-664.e19"
COMMENT     Sequence Label: pBEL1892
FEATURES             Location/Qualifiers
     source          1..7040
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     222..241
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        222..240
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             272..294
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(4219..4237)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4233..4280
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4325..4343)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4411..4515
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4516..4584,4585..5376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4734..4753)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5547..6135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6036..6055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6289..6306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6321..6460
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6386..6405)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6546..6568
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6562..6753)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt
       61 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
      121 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
      181 gccaggaccc aacgctgccc gagatctcga tcccgcgaaa ttaatacgac tcactatagg
      241 gagaccacaa cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatataca
      301 tatggctctt aacacaccac aaattacgcc gacaaaaaag ataacagtga gggcaatcgg
      361 cgaggaactg ccgcgtggtg attaccaacg ttgcccgcaa tgtgacatgc tgtttagcct
      421 gcccgagata aattctcatc aaagtgccta ttgtccgcgc tgtcaggcaa aaattcgtga
      481 cgggcgcgac tggtcgctaa cgcgcctggc ggcaatggcc ttcaccatgc tgttgcttat
      541 gccgtttgcc tggggcgaac cgctgttgca tatctggctg ttaggcattc gtattgacgc
      601 caacgttatg caaggcatct ggcaaatgac caaacagggc gatgcgataa cggggtcgat
      661 ggtctttttc tgcgttattg gtgccccgct cattctggtg acctccatag cttatttatg
      721 gtttggtaac cgactgggaa tgaatttacg tccggtactg ctgatgcttg agcgacttaa
      781 agagtgggta atgctcgata tctacctggt cggcattggc gttgcttcta taaaggtaca
      841 ggattatgcc catatccagg ctggtgtcgg cttgttctct tttgtggcgt tggtgatttt
      901 aacgacggtg acgttgtcac atcttaatgt cgaagaactg tgggagcgat tttatccgca
      961 gcgccccgct acgcgtaggg acgagaaact tcgtgtctgc ctagggtgcc attttaccgg
     1021 ctatccagat cagcgtggtc gctgcccgcg ttgccatatc ccgctacgcc tgcgtcgccg
     1081 tcatagtctg caaaaatgct gggcggcgct gttagcgtca atcgttttat tgttacctgc
     1141 caacctgttg cctatttcta tcatttatct gaacggagga cggcaggaag atacaattct
     1201 ttccggaatt atgtcgctgg caagtagcaa cattgcggtt gcaggaatcg tgtttatcgc
     1261 cagtattctg gtcccgttta ctaaagtgat cgtcatgttc actttactgt tgagcattca
     1321 ttttaaatgc cagcaaggtt tacgcacacg cattctgtta ctgcggatgg tgacctggat
     1381 tggtcgctgg tcgatgctcg acctgtttgt catatcttta accatgtcgc tgattaatcg
     1441 cgatcagatc ctcgctttta ctatgggacc ggctgcgttt tatttcggcg cagcggtaat
     1501 tttgactatt cttgctgtgg aatggctgga cagccgctta ctttgggatg cacatgagtc
     1561 aggaaacgcc cgcttcgacg actgaagcgc agattaaaaa taaacgccgt atctcacctt
     1621 tctggctgct gccgttcatc gcgctaatga ttgccagttg gctgatttgg gacagttatc
     1681 aggaccgggg taataccgtc accatcgact ttatgtcggc ggatggtatt gttccaggcc
     1741 gtacgcctgt tcgttatcag ggcgttgaag tcggaacagt gcaggacatc agcctcagcg
     1801 acgatcttcg taagattgaa gtcaaggtca gcatcaagtc cgatatgaaa gatgcgctgc
     1861 gcgaagagac tcagttctgg ctggtgacgc caaaagcatc gttggcaggt gtctccgggc
     1921 tggacgccct cgtcggtggg aactatatcg gcatgatgcc cgggaaaggt aaagagcagg
     1981 atcactttgt cgcactcgat acccaaccga aatatcggct ggacaatggc gatctgatga
     2041 tccacctgca agcccccgat ctcggttcgc tgaacagcgg ttcattggtc tatttccgca
     2101 agatcccggt gggaaaagtc tacgactatg ccatcaatcc caacaagcaa ggcgtggtga
     2161 ttgatgtcct gatcgagcgg cgttttaccg atctggtgaa aaaaggtagc cgtttctgga
     2221 acgtttccgg cgttgatgcc aacgtcagta tcagtggcgc gaaggtgaaa ctggaaagct
     2281 tggcggcact ggttaacggt gcgattgcct tcgattcacc agaagagtcg aaacctgccg
     2341 aggcggaaga tacctttggt ctgtatgaag atctggccca cagccagcgt ggcgtaataa
     2401 taaaactgga actgccgagt ggggccggat taaccgccga ctcgacgccg ttaatgtatc
     2461 aggggctgga agtcggacag ctgactaaac tggatttaaa tcctggtggt aaagtcacag
     2521 gggaaatgac cgttgatccc agcgtcgtta ccctgcttcg tgaaaatacc cgcatcgaat
     2581 tacgcaaccc gaaattatcc cttagcgatg ctaatctcag cgccctgctg accggcaaaa
     2641 ccttcgagct ggtgcccggc gatggcgagc cacgcaaaga attcgttgtt gtgccaggcg
     2701 aaaaagcact gctgcatgaa cctgatgttc tgacgctgac cctgaccgcg ccggaaagtt
     2761 acggtattga tgcgggtcag ccgctcattc ttcacggcgt gcaggtaggc caggttatcg
     2821 atcgtaaact caccagcaaa ggcgtcacct ttaccgtcgc catcgagcct cagcatcgcg
     2881 aactggtaaa aggcgatagc aaatttgtcg tcaacagccg tgtcgatgtg aaggtggggc
     2941 tggatggcgt tgagtttctc ggtgccagcg cctcagaatg gatcaatggc gggatccgta
     3001 ttctgccggg cgataaaggt gagatgaaag ccagctatcc actgtatgcc aatttggaaa
     3061 aagcgctgga gaacagcctt agcgatttac ccaccacaac cgtgagtttg agtgcagaga
     3121 cgctgccgga tgtgcaggca ggatcggtag tgctgtaccg taaatttgaa gttggtgaag
     3181 tgattaccgt gcgtccgcga gctaacgcgt ttgacatcga tctgcatatt aagccggagt
     3241 atcgcaacct tctgaccagc aatagcgtgt tctgggcaga aggcggggcg aaagttcagc
     3301 tgaatggtag tggtctgacc gtacaggcat ccccgctctc cagagctcta aagggagcca
     3361 ttagcttcga caacctcagc ggtgccagcg ccagtcagcg taaaggcgat aagcgtattc
     3421 tgtatgcttc cgaaacagcg gcccgtgcgg ttggcgggca gattacgctt cacgctttcg
     3481 atgccggaaa actggcggtc gggatgccaa ttcgctatct cggtattgac atcgggcaaa
     3541 tccagacgct ggatctgatt accgcacgca atgaagtaca ggcaaaggcg gtactttacc
     3601 cggaatatgt ccagaccttt gctcgcggtg gtacgcgctt ctcagtggtc acaccgcaaa
     3661 tttcggcagc tggcgttgag catcttgata ctatcctcca gccgtatatc aacgtcgaac
     3721 caggccgggg caatcctcgc cgcgactttg aattacaaga ggccaccatt actgattcgc
     3781 ggtacctgga tggcttaagc attattgttg aagcgccgga agccggttcg ttaggcatcg
     3841 gtacgcctgt gctgttccgt ggtctggaag tcggtacggt tacaggaatg acgctgggga
     3901 cattgtcaga tcgcgtgatg attgcgatgc gcatcagtaa acgctatcaa cacctggtgc
     3961 gtaacaattc cgtcttctgg ttggcatcgg gttacggtgg cggcaccttt aaccagttta
     4021 tccgtggcgg catcgccttc gccacgcctc cgggtacgcc actggcaccg aaagcccagg
     4081 aaggcaaaca cttcctgttg caggaaagtg aaccgaaaga gtggcgtgaa tggggaactg
     4141 cgcttcccaa ataatgccca cctcgagcag atccggctgc taacaaagcc cgaaaggaag
     4201 ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac
     4261 gggtcttgag gggttttttg ctgaaaggag gaactatatc cggataattc ttgaagacga
     4321 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     4381 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4441 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4501 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4561 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4621 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4681 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4741 ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat
     4801 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4861 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4921 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4981 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5041 cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5101 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5161 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5221 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5281 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5341 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5401 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5461 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5521 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5581 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5641 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
     5701 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5761 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5821 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5881 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5941 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6001 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6061 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6121 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6181 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     6241 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     6301 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6361 tcacaccgca atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     6421 atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc
     6481 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6541 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca
     6601 gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc
     6661 cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc
     6721 catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct
     6781 gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga
     6841 tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg
     6901 gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg
     6961 tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg
     7021 cgctgacttc cgcgtttcca
//
