LOCUS       sequence-309762-        1794 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Newcastle disease virus HN protein (47-575) with 
            C-terminal SpyTag fusion in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Rahikainen R, Rijal P, Tan TK, Wu HJ, Andersson AC, Barrett JR, 
            Bowden TA, Draper SJ, Townsend AR, Howarth M
  TITLE     Overcoming Symmetry Mismatch in Vaccine Nanoassembly through 
            Spontaneous Amidation.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663.
  PUBMED    32886840
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Sep 4. doi: 10.1002/anie.202009663."
COMMENT     Sequence Label: NDV-HN-SpyTag
FEATURES             Location/Qualifiers
     source          1..1794
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1714..1752
                     /codon_start=1
                     /product="peptide derived from the CnaB2 adhesin domain of 
                     Streptococcus pyogenes FbaB"
                     /label=SpyTag
                     /note="forms a covalent isopeptide bond with the SpyCatcher
                     protein (Zakeri et al., 2012)"
                     /translation="AHIVMVDAYKPTK"
     CDS             1771..1788
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggggatcc ttcccagccc tgggatgcct gcgctgctct ccctcgtgag ccttctctcc
       61 gtgctgctga tgggttgcgt agctgaaacc ggtagcatgg gcgccagcac accttctgat
      121 ctcgtgggca tccccaccag aatcagcaga gccgaggaaa agatcacaag cgccctgggc
      181 agcaaccagg acgtggtgga cagaatctac aagcaggtcg ccctcgagag ccctctggca
      241 ctgctgaata ccgagacaac catcatgaac gccatcacca gcctgagcta ccagatcaac
      301 ggcgctgcca acaattctgg ctggggcgct cctatccacg atcctgattt catcggcggc
      361 atcggcaaag aactgatcgt ggacaatgcc agcgacgtga ccagcttcta ccccagcgcc
      421 tttcaagagc acctgaactt catccccgct cctacaacag gcagcggctg cacaagaatc
      481 cccagcttcg atatgagcgc cacacactac tgctacaccc acaacgtgat cctgagcggc
      541 tgtagggatc acagccacag ccatcagtat ctggccctgg gcgtgctgag aacaaccgcc
      601 acaggccgga tctttttcag caccctgaga tccatcagcc tggacgacac ccagaaccgg
      661 aagtcctgta gcgtgtcagc cacacctctg ggctgcgata tgctgtgcag caaagtgacc
      721 gagacagagg aagaggacta caacagcgcc gtgcctacac tgatggccca tggcagactg
      781 ggctttgacg gccagtacca cgagaaggac ctggacgtga caaccctgtt cgaggactgg
      841 gtcgccaact atcctggtgt tggcggcgga agcttcatcg acggcagagt gtggtttagc
      901 gtgtacggcg gcctgaagcc taacagccct agcgatacag tccaagaggg caaatacgtg
      961 atctataagc ggtacaacga cacatgcccc gacgagcagg attaccagat ccggatggct
     1021 aagagcagct acaagcccgg cagattcggc ggcaagagaa tccagcaggc catcctgagc
     1081 atcaaggtgt ccacaagcct gggcgaagat cccgtgctga cagtgcctcc taacaccgtg
     1141 actctgatgg gcgccgaggg cagaatcctg acagtgggca caagccactt tctgtaccag
     1201 cggggcagca gctacttcag ccctgctctg ctgtacccca tgaccgtgtc caacaagacc
     1261 gccacactgc acagccccta caccttcaac gcctttacca gacctggcag catcccctgt
     1321 caggcctctg ccagatgtcc caatagctgt gtgaccggcg tgtacacaga cccctatcct
     1381 ctgatcttct accggaatca caccctgagg ggcgtgttcg gcacaatgct ggatagcgaa
     1441 caggccagac tgaaccctgc ctccgccgtg tttgatagca ccagcagatc ccggatcacc
     1501 agagtgtcta gcagcagcac aaaggccgcc tataccacca gcacctgttt caaggtggtc
     1561 aagacaaaca agacctactg cctgtctatc gccgagatca gcaacaccct gtttggcgag
     1621 ttcagaatcg tgcccctgct ggtggaaatc ctgaagaacg atggcgtgcg cgaggccaga
     1681 agcggaggct ccggagggtc aggcggatct ggggctcata ttgttatggt tgatgcttac
     1741 aagccaacta agggaagcgg gggtaccaag caccaccatc accatcacta atga
//
