LOCUS       sequence-309790-       10000 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pcDNA5 transfection plasmid for the exogenous expression of 
            N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10000)
  AUTHORS   Gonatopoulos-Pournatzis T, Niibori R, Salter EW, Weatheritt RJ, 
            Tsang B, Farhangmehr S, Liang X, Braunschweig U, Roth J, Zhang S, 
            Henderson T, Sharma E, Quesnel-Vallieres M, Permanyer J, Maier S, 
            Georgiou J, Irimia M, Sonenberg N, Forman-Kay JD, Gingras AC, 
            Collingridge GL, Woodin MA, Cordes SP, Blencowe BJ
  TITLE     Autism-Misregulated eIF4G Microexons Control Synaptic Translation 
            and Higher Order Cognitive Functions.
  JOURNAL   Mol Cell. 2020 Mar 19;77(6):1176-1192.e16. doi: 
            10.1016/j.molcel.2020.01.006. Epub 2020 Jan 29.
  PUBMED    31999954
REFERENCE   2  (bases 1 to 10000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2020.01.006"; journalName: "Mol Cell"; date: 
            "2020-03-19- 19"; volume: "77"; issue: "6"; pages: "1176-1192.e16"
COMMENT     Sequence Label: pcDNA5 CMV 3xFG eIF4G1
FEATURES             Location/Qualifiers
     source          1..10000
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    586..604
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    607..625
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     635..659
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             800..823
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    824..848
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(5652..5676)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(5718..5735)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5724..5948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6081..6100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     protein_bind    6232..6279
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             6287..7309
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     polyA_signal    7439..7560
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7476..7495)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7530..7549
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7609..7625)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7609..7625)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7622..7644)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7633..7649
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7657..7663,7664..7681,7682..7687))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7702..7723
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7840..7857)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8011..8599)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8091..8110)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8770..9561,9562..9630))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9393..9412
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9631..9735)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9810..9829)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
      601 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
      661 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
      721 tccggactct agcgtttaaa cttaagcttc caccatggac tacaaagacc atgacggtga
      781 tacatatgat catgacatcg attacaagga tgacgatgac aagacaagtt tgtacaaaaa
      841 agcaggcttc aacaaagctc cacagtccac aggcccccca cccgccccat cccccggact
      901 cccacagcca gcgtttcccc cggggcagac agcgccggtg gtgttcagta cgccacaagc
      961 gacacaaatg aacacgcctt ctcagccccg ccagcacttc taccctagcc gggcccagcc
     1021 cccgagcagt gcagcctccc gagtgcagag tgcagcccct gcccgccctg gcccagctgc
     1081 ccatgtctac cctgctggat cccaagtaat gatgatccct tcccagatct cctacccagc
     1141 ctcccagggg gcctactaca tccctggaca ggggcgttcc acatacgttg tcccgacaca
     1201 gcagtaccct gtgcagccag gagccccagg cttctatcca ggtgcaagcc ctacagaatt
     1261 tgggacctac gctggcgcct actatccagc ccaaggggtg cagcagtttc ccactggcgt
     1321 ggcccccgcc ccagttttga tgaaccagcc accccagatt gctcccaaga gggagcgtaa
     1381 gacgatccga attcgagatc caaaccaagg aggaaaggat atcacagagg agatcatgtc
     1441 tggggcccgc actgcctcca cacccacccc tccccagacg ggaggcggtc tggagcctca
     1501 agctaatggg gagacgcccc aggttgctgt cattgtccgg ccagatgacc ggtcacaggg
     1561 agcaatcatt gctgaccggc cagggctgcc tggcccagag catagccctt cagaatccca
     1621 gccttcgtcg ccttctccga ccccatcacc atccccagtc ttggaaccgg ggtctgagcc
     1681 taatctcgca gtcctctcta ttcctgggga cactatgaca actatacaaa tgtctgtaga
     1741 agaatcaacc cccatctccc gtgaaactgg ggagccatat cgcctctctc cagaacccac
     1801 tcctctcgcc gaacccatac tggaagtaga agtgacactt agcaaaccgg ttccagaatc
     1861 tgagttttct tccagtcctc tccaggctcc cacccctttg gcatctcaca cagtggaaat
     1921 tcatgagcct aatggcatgg tcccatctga agatctggaa ccagaggtgg agtcaagccc
     1981 agagcttgct cctcccccag cttgcccctc cgaatcccct gtgcccattg ctccaactgc
     2041 ccaacctgag gaactgctca acggagcccc ctcgccacca gctgtggact taagcccagt
     2101 cagtgagcca gaggagcagg ccaaggaggt gacagcatca gtggcgcccc ccaccatccc
     2161 ctctgctact ccagctacgg ctccttcagc tacttcccca gctcaggagg aggaaatgga
     2221 agaagaagaa gaagaggaag aaggagaagc aggagaagca ggagaagctg agagtgagaa
     2281 aggaggagag gaactgctcc ccccagagag tacccctatt ccagccaact tgtctcagaa
     2341 tttggaggca gcagcagcca ctcaagtggc agtatctgtg ccaaagagga gacggaaaat
     2401 taaggagcta aataagaagg aggctgttgg agaccttctg gatgccttca aggaggcgaa
     2461 cccggcagta ccagaggtgg aaaatcagcc tcctgcaggc agcaatccag gcccagagtc
     2521 tgagggcagt ggtgtgcccc cacgtcctga ggaagcagat gagacctggg actcaaagga
     2581 agacaaaatt cacaatgctg agaacatcca gcccggggaa cagaagtatg aatataagtc
     2641 agatcagtgg aagcctctaa acctagagga gaaaaaacgt tacgaccgtg agttcctgct
     2701 tggttttcag ttcatctttg ccagtatgca gaagccagag ggattgccac atatcagtga
     2761 cgtggtgctg gacaaggcca ataaaacacc actgcggcca ctggatccca ctagactaca
     2821 aggcataaat tgtggcccag acttcactcc atcctttgcc aaccttggcc ggacaaccct
     2881 tagcacccgt gggcccccaa ggggtgggcc aggtggggag ctgccccgtg ggccggctgg
     2941 cctgggaccc cggcgctctc agcagggacc ccgaaaagaa ccacgcaaga tcattgccac
     3001 agtgttaatg accgaagata taaaactgaa caaagcagag aaagcctgga aacccagcag
     3061 caagcggacg gcggctgata aggatcgagg ggaagaagat gctgatggca gcaaaaccca
     3121 ggacctattc cgcagggtgc gctccatcct gaataaactg acaccccaga tgttccagca
     3181 gctgatgaag caagtgacgc agctggccat cgacaccgag gaacgcctca aaggggtcat
     3241 tgacctcatt tttgagaagg ccatttcaga gcccaacttc tctgtggcct atgccaacat
     3301 gtgccgctgc ctcatggcgc tgaaagtgcc cactacggaa aagccaacag tgactgtgaa
     3361 cttccgaaag ctgttgttga atcgatgtca gaaggagttt gagaaagaca aagatgatga
     3421 tgaggttttt gagaagaagc aaaaagagat ggatgaagct gctacggcag aggaacgagg
     3481 acgcctgaag gaagagctgg aagaggctcg ggacatagcc cggcggcgct ctttagggaa
     3541 tatcaagttt attggagagt tgttcaaact gaagatgtta acagaggcaa taatgcatga
     3601 ctgtgtggtc aaactgctta agaaccatga tgaagagtcc cttgagtgcc tttgtcgtct
     3661 gctcaccacc attggcaaag acctggactt tgaaaaagcc aagccccgaa tggatcagta
     3721 tttcaaccag atggaaaaaa tcattaaaga aaagaagacg tcatcccgca tccgctttat
     3781 gctgcaggac gtgctggatc tgcgagggag caattgggtg ccacgccgag gggatcaggg
     3841 tcccaagacc attgaccaga tccataagga ggctgagatg gaagaacatc gagagcacat
     3901 caaagtgcag cagctcatgg ccaagggcag tgacaagcgt cggggcggtc ctccaggccc
     3961 tcccatcagc cgtggacttc cccttgtgga tgatggtggc tggaacacag ttcccatcag
     4021 caaaggtagc cgccccattg acacctcacg actcaccaag atcaccaagc ctggctccat
     4081 cgattctaac aaccagctct ttgcacctgg agggcgactg agctggggca agggcagcag
     4141 cggaggctca ggagccaagc cctcagacgc agcatcagaa gctgctcgcc cagctactag
     4201 tactttgaat cgcttctcag cccttcaaca agcggtaccc acagaaagca cagataatag
     4261 acgtgtggtg cagaggagta gcttgagccg agaacgaggc gagaaagctg gagaccgagg
     4321 agaccgccta gagcggagtg aacggggagg ggaccgtggg gaccggcttg atcgtgcgcg
     4381 gacacctgct accaagcgga gcttcagcaa ggaagtggag gagcggagta gagaacggcc
     4441 ctcccagcct gaggggctgc gcaaggcagc tagcctcacg gaggatcggg accgtgggcg
     4501 ggatgccgtg aagcgagaag ctgccctacc cccagtgagc cccctgaagg cggctctctc
     4561 tgaggaggag ttagagaaga aatccaaggc tatcattgag gaatatctcc atctcaatga
     4621 catgaaagag gcagtccagt gcgtgcagga gctggcctca ccctccttgc tcttcatctt
     4681 tgtacggcat ggtgtcgagt ctacgctgga gcgcagtgcc attgctcgtg agcatatggg
     4741 gcagctgctg caccagctgc tctgtgctgg gcatctgtct actgctcagt actaccaagg
     4801 gttgtatgaa atcttggaat tggctgagga catggaaatt gacatccccc acgtgtggct
     4861 ctacctagcg gaactggtaa cacccattct gcaggaaggt ggggtgccca tgggggagct
     4921 gttcagggag attacaaagc ctctgagacc gttgggcaaa gctgcttccc tgttgctgga
     4981 gatcctgggc ctcctgtgca aaagcatggg tcctaaaaag gtggggacgc tgtggcgaga
     5041 agccgggctt agctggaagg aatttctacc tgaaggccag gacattggtg cattcgtcgc
     5101 tgaacagaag gtggagtata ccctgggaga ggagtcggaa gcccctggcc agagggcact
     5161 cccctccgag gagctgaaca ggcagctgga gaagctgctg aaggagggca gcagtaacca
     5221 gcgggtgttc gactggatag aggccaacct gagtgagcag cagatagtat ccaacacgtt
     5281 agttcgagcc ctcatgacgg ctgtctgcta ttctgcaatt atttttgaga ctcccctccg
     5341 agtggacgtt gcagtgctga aagcgcgagc gaagctgctg cagaaatacc tgtgtgacga
     5401 gcagaaggag ctacaggcgc tctacgccct ccaggccctt gtagtgacct tagaacagcc
     5461 tcccaacctg ctgcggatgt tctttgacgc actgtatgac gaggacgtgg tgaaggagga
     5521 tgccttctac agttgggaga gtagcaagga ccccgctgag cagcagggca agggtgtggc
     5581 ccttaaatct gtcacagcct tcttcaagtg gctccgtgaa gcagaggagg agtctgacca
     5641 caactgatga gacccagctt tcttgtacaa agtggtctga ctcgagtcta gagggcccgt
     5701 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5761 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5821 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5881 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5941 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     6001 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6061 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6121 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6181 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtacct agaagttcct
     6241 attccgaagt tcctattctc tagaaagtat aggaacttcc ttggccaaaa agcctgaact
     6301 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     6361 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     6421 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     6481 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggagt tcagcgagag
     6541 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     6601 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     6661 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     6721 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     6781 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     6841 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     6901 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     6961 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     7021 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     7081 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     7141 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     7201 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     7261 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaatagc acgtactacg
     7321 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
     7381 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
     7441 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     7501 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     7561 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt
     7621 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     7681 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     7741 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     7801 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     7861 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     7921 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     7981 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8041 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8101 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8161 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8221 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8281 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8341 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8401 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     8461 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8521 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8581 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8641 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8701 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8761 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     8821 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     8881 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     8941 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9001 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9061 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9121 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9181 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9241 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9301 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9361 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9421 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9481 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9541 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9601 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9661 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9721 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga
     9781 tctcccgatc ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9841 cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa
     9901 gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt
     9961 tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
//
