LOCUS       sequence-309881-       10385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Set of empty Gateway Cloning compatible, inducible, lentiviral 
            vectors with various mammalian selection markers and N-terminal 
            fluorescent protein fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Melore S, Chavez A
  TITLE     Chavez lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLIX403-iRFP-ccdB-Puro
FEATURES             Location/Qualifiers
     source          1..10385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..315
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    9..27
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    45..63
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    80..98
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    116..134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    152..170
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     288..312
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             332..1279
                     /codon_start=1
                     /product="phytochrome-based near-infrared fluorescent 
                     protein, originally termed iRFP (Filonov et al., 2011)"
                     /label=iRFP713
                     /note="name was changed to iRFP713 (Shcherbakova and 
                     Verkhusha, 2013)"
                     /translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
                     DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
                     GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
                     GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
                     NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPVHLEFMRNIGMHGTMSISILRGERLWGL
                     IVCHHRTPYYVDLDGRQACELVAQVLAWQIGVMEE"
     protein_bind    1310..1434
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1459..1464,1465..1482,1483..1489)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1543..2202
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1609..1628)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2544..2849
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2728..2747
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2890..3014)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3019..3060
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3074..3091
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3101..3611
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3530..3548
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     primer_bind     complement(3627..3646)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4123..4143
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4233..4286
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4287..4904,4905..4910,4911..4946,4947..4949,4950..4985,
                     4986..4988,4989..5024,5025..5033)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5073..5093)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5491..5502)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5707..5940
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6012..6133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6049..6068)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6103..6122
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6173..6308
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6308..6379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6380..6448,6449..7240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6598..6617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7411..7999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7900..7919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8153..8170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8287..8308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8323..8328,8329..8346,8347..8353)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8361..8377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8366..8388
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8385..8401
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8385..8401
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8420..8440
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8422..8440
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8468..8694
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8695..8875
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8922..9047
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9540..9773
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9958..10002
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10215..10332
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcctggag aattggctag catggcggaa ggatccgtcg ccaggcagcc
      361 tgacctcttg acctgcgacg atgagccgat ccatatcccc ggtgccatcc aaccgcatgg
      421 actgctgctc gccctcgccg ccgacatgac gatcgttgcc ggcagcgaca accttcccga
      481 actcaccgga ctggcgatcg gcgccctgat cggccgctct gcggccgatg tcttcgactc
      541 ggaaacgcac aaccgtctga cgatcgcctt ggccgagccc ggggcggccg tcggagcacc
      601 gatcactgtc ggcttcacga tgcgaaagga cgcaggcttc atcggctcct ggcatcgcca
      661 tgatcagctc atcttcctcg agctcgagcc tccccagcgg gacgtcgccg agccgcaggc
      721 gttcttccgc cgcaccaaca gcgccatccg ccgcctgcag gccgccgaaa ccttggaaag
      781 cgcctgcgcc gccgcggcgc aagaggtgcg gaagattacc ggcttcgatc gggtgatgat
      841 ctatcgcttc gcctccgact tcagcggcga agtgatcgca gaggatcggt gcgccgaggt
      901 cgagtcaaaa ctaggcctgc actatcctgc ctcaaccgtg ccggcgcagg cccgtcggct
      961 ctataccatc aacccggtac ggatcattcc cgatatcaat tatcggccgg tgccggtcac
     1021 cccagacctc aatccggtca ccgggcggcc gattgatctt agcttcgcca tcctgcgcag
     1081 cgtatcgccc gtccatctgg agttcatgcg caacataggc atgcacggca cgatgtcgat
     1141 ctcgattttg cgcggcgagc gactgtgggg attgatcgtt tgccatcacc gaacgccgta
     1201 ctacgtcgat ctcgatggcc gccaagcctg cgagctagtc gcccaggttc tggcctggca
     1261 gatcggcgtg atggaagagg gggccggcca tatcagcggc tctggttcga caagtttgta
     1321 caaaaaagct gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     1381 gcataaaaaa cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc
     1441 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
     1501 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
     1561 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
     1621 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
     1681 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
     1741 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
     1801 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
     1861 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
     1921 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1981 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     2041 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     2101 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     2161 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     2221 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     2281 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     2341 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     2401 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     2461 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     2521 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     2581 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     2641 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     2701 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     2761 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     2821 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     2881 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     2941 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     3001 ttgtacaaag tggttgcagg aaagccaatc ccaaacccac tcctaggtct cgacagtaca
     3061 gcatagaccg gtccaccacc accaccacca ctaaggatcc ggggttgggg ttgcgccttt
     3121 tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt tccgggaaac
     3181 gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc gtcacccgga
     3241 tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc ctaagtcggg
     3301 aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca cgtctcacta
     3361 gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg cgatgggctg
     3421 tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa ggggcggtgc
     3481 gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg tgttccgcat
     3541 tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga atcaccgacc
     3601 tctctcccca gcttaattaa ttcaccatga ccgagtacaa gcccacggtg cgcctcgcca
     3661 cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
     3721 ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
     3781 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
     3841 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
     3901 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
     3961 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
     4021 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
     4081 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
     4141 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
     4201 gcatgacccg caagcctgca ggtcccggtg ccgaaggtag aggttctctc ctcacttgtg
     4261 gtgatgttga agaaaaccct ggtccaatgt ctagactgga caagagcaaa gtcataaacg
     4321 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     4381 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     4441 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     4501 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     4561 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     4621 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     4681 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     4741 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     4801 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     4861 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     4921 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgacttt gaccttgata
     4981 tgctgcctgc tgacgctctt gacgattttg accttgacat gctccccggg taactaagta
     5041 aggatcgatc caagatatcg tattcttaac tatgttgctc cttttacgct atgtggatac
     5101 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     5161 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     5221 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     5281 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     5341 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     5401 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     5461 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     5521 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5581 cggatctccc tttgggccgc ctccccgcct gtttcgcctc ggcgtccgga ctagaggtac
     5641 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5701 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5761 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5821 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5881 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5941 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6001 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6061 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6121 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6181 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6241 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6301 tggaggcctt tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6361 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6421 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6481 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6541 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6601 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6661 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6721 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6781 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6841 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6901 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6961 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     7021 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     7081 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     7141 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7201 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7261 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7321 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7381 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7441 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7501 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7561 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7621 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7681 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7741 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7801 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7861 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7921 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7981 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8041 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8101 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8161 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8221 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8281 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8341 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8401 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     8461 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     8521 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
     8581 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     8641 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     8701 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8761 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8821 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     8881 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     8941 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9001 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9061 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9121 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9181 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     9241 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     9301 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     9361 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     9421 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     9481 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     9541 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     9601 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     9661 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     9721 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     9781 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     9841 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     9901 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     9961 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    10021 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    10081 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    10141 tcgtttcaga cccacctccc aaccccgagg ggacaattct cgacctcgag acaaatggca
    10201 gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
    10261 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
    10321 attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc gcgaatcgat
    10381 atgtc
//
