LOCUS       sequence-309885-        1113 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 0.5 Golden Gate Vector, loxP-mCHERRY-HDEL-t35s-loxP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Harrington SA, Backhaus AE, Fox S, Rogers C, Borrill P, Uauy C, 
            Richardson A
  TITLE     A heat-shock inducible system for flexible gene expression in 
            cereals.
  JOURNAL   Plant Methods. 2020 Oct 14;16:137. doi: 10.1186/s13007-020-00677-3. 
            eCollection 2020.
  PUBMED    33072173
REFERENCE   2  (bases 1 to 1113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2020-10-14"; volume: "16:137. doi"; pages: " 
            10.1186/s13007-020-00677-3. eCollection 2020"
COMMENT     Sequence Label: EC71022
FEATURES             Location/Qualifiers
     source          1..1113
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(7..40)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             125..829
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(267..285)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     polyA_signal    885..1059
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     protein_bind    complement(1069..1102)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt gaatgaaaac
       61 taatcttttt ctctttctca tcttttcact tctcctatca ttatcctcgg ccgaattcgg
      121 aggtgtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
      181 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      241 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
      301 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
      361 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
      421 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
      481 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
      541 aatgcagaag aaaaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
      601 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
      661 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
      721 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
      781 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg gtggccatga
      841 tgagctttaa gcttctctag ctagagtcga tcgacaagct cgagtttctc cataataatg
      901 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
      961 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
     1021 taattcctaa aaccaaaatc cagtactaaa atccagatcg ctgacctaat aacttcgtat
     1081 agcatacatt atacgaagtt atattaaggg ttg
//
