LOCUS       sequence-309978-       11036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK17 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11036)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK17_IDG-K
FEATURES             Location/Qualifiers
     source          1..11036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(161..229,230..1021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1192..1780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1681..1700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1934..1951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2068..2089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2104..2109,2110..2127,2128..2134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2142..2158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2147..2169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2166..2182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2166..2182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2201..2221
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2203..2221
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2249..2475
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2476..2656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2703..2828
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3321..3554
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3739..3783
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4050..4166
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4200..4220
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4220..5431
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5268..5287
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5435..5455)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      5456..5465
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5468..6238
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             6245..6286
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    6287..6311
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7890..7914)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7944..8532
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7997..8017)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8415..8426)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8546..9045
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8581..8604)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8978..8997
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9066..9665
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9066..9085)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9562..9582
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             9803..10036
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10108..10229
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10145..10164)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10199..10218
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10269..10404
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10424..10443)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10425..10443)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10453..10470)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10453..10469)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10462..10484)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(10611..11036,1..3)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10698..10717)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10908..10929
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
       61 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      121 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      181 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      241 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      301 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      361 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      421 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      481 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      541 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      601 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      661 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      721 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      781 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
      841 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      901 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      961 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1021 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1081 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1141 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1801 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1861 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1921 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1981 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2041 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2101 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2161 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     2221 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     2281 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     2341 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     2401 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     2461 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2641 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     2701 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     2761 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2821 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     2881 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     2941 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     3001 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     3061 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     3121 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     3181 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     3241 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     3301 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     3361 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     3421 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     3481 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     3541 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     3601 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     3661 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     3721 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3781 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3841 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     3901 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     3961 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     4021 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
     4081 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     4141 aaacaaatta caaaattcaa aattttatcg tccagtgtgg tggaattctg cagatatcac
     4201 aactttgtat agaaaagttg gcctccgcgc cgggttttgg cgcctcccgc gggcgccccc
     4261 ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc tgatccttcc
     4321 gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga accccagtat
     4381 cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt tttctttcca
     4441 gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga gggatctccg
     4501 tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca gctagttccg
     4561 tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg tcacttggtg
     4621 agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc ggtgggacgg
     4681 aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg ttgccctgaa
     4741 ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga atggaagacg
     4801 cttgtgaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg gcggcaagaa
     4861 cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga gatgggctgg
     4921 ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc ggtttgtcgt
     4981 ctgttgcggg ggcggcagtt atggcggtgc cgttgggcag tgcacccgta cctttgggag
     5041 cgcgcgccct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg cagggtgggg
     5101 ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg gttcgggcct
     5161 agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg gataagtgag
     5221 gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga agctccggtt
     5281 ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg caccttttga
     5341 aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat tgtccgctaa
     5401 attctggccg tttttggctt ttttgttaga cggccaactt ttgtatacaa agtgggccac
     5461 catgggaatc ccgctgctga acgctaaaca gattctggga cagctggacg gcgggagcgt
     5521 ggcagtcctg cctgtggtcg actccaccaa tcagtacctg ctggatcgaa tcggcgagct
     5581 gaagagtggg gatgcttgca ttgcagaata tcagcaggca gggagaggaa gcagagggag
     5641 gaaatggttc tctccttttg gagctaacct gtacctgagt atgttttggc gcctgaagcg
     5701 gggaccagca gcaatcggcc tgggcccggt catcggaatt gtcatggcag aagcgctgcg
     5761 aaagctggga gcagacaagg tgcgagtcaa atggcccaat gacctgtatc tgcaggatag
     5821 aaagctggca ggcatcctgg tggagctggc cggaataaca ggcgatgctg cacagatcgt
     5881 cattggcgcc gggattaacg tggctatgag gcgcgtggag gaaagcgtgg tcaatcaggg
     5941 ctggatcaca ctgcaggaag cagggattaa cctggacagg aatactctgg ccgctatgct
     6001 gatccgagag ctgcgggcag ccctggaact gttcgagcag gaaggcctgg ctccatatct
     6061 gtcacggtgg gagaagctgg ataacttcat caatagaccc gtgaagctga tcattgggga
     6121 caaagagatt ttcgggatta gccgggggat tgataaacag ggagccctgc tgctggaaca
     6181 ggacggagtt atcaaaccct ggatgggcgg agaaatcagt ctgcggtctg ccgaaaaggc
     6241 tagcggcaag cccatcccca accccctgct gggcctggac agcaccacaa ctttgtacaa
     6301 aaaagcaggc tccaccatga aaaaatttaa gagaaggcta tccctcacac tccgaggaag
     6361 tcagactatt gatgaatcat tgtctgaatt ggctgaacaa atgactattg aagaaaacag
     6421 cagcaaggat aatgagccta ttgtgaagaa tggcaggcct ccaacgtctc acagtatgca
     6481 ttccttcctc caccagtaca caggatcttt caagaagccc ccattgcgga gaccacacag
     6541 tgttattgga gggagccttg gctccttcat ggcaatgccc agaaatggaa gcagattaga
     6601 tattgttcat gaaaatctaa aaatgggatc agatggtgag agtgaccaag cttctgggac
     6661 atcatctgat gaagtccagt cacctacagg tgtttgtctc agaaatcgta tacatagacg
     6721 gatctcaatg gaggatttaa ataagcggtt atcactgcct gcagacatca gaatacctga
     6781 tggatatctt gaaaagttgc agataaacag tccaccattt gaccaaccaa tgagtcgaag
     6841 gtctcgtaga gcttccttat cagaaattgg ctttggaaaa atggaaacct acatcaaatt
     6901 ggaaaagctt ggagagggta catatgcaac agtatataaa ggaagaagta aattgacaga
     6961 gaatttggtg gcattaaaag agatccgatt ggaacatgaa gaaggtgcac cctgcacagc
     7021 tataagagaa gtttcactat taaaggattt aaaacatgca aatatagtaa ccttacatga
     7081 cattgttcac acagataaat ccttgacttt ggtgtttgag tatctggata aagacctgaa
     7141 acagtacatg gatgactgtg gaaacatcat gagtatgcac aacgtaaagc tgtttctgta
     7201 ccaaattcta cgtggtttgg catattgcca tagaagaaag gtattgcatc gagacttgaa
     7261 accacagaac ctcctcatta atgagaaagg agaattaaag ctagcagatt ttggactagc
     7321 ccgagccaag tcagttccca caaagaccta ctcaaatgaa gttgtcacac tatggtaccg
     7381 gccacctgat gtgcttcttg gttcctcgga gtactcaaca cagattgaca tgtggggtgt
     7441 tggttgcatt ttctttgaaa tggcttctgg aagaccttta tttccaggat caaccgtgga
     7501 agatgaactg cacttaattt tccgactgct aggaactcca tctcaggaaa cttggccagg
     7561 tatttcttca aatgaggagt tcaagaacta caactttcca aaatataaac cacagcctct
     7621 aattaaccac gcacccaggt tagactctga aggaattgag ttgataacaa aatttcttca
     7681 gtatgaatct aagaaaaggg tttcagctga agaggccatg aaacatgtgt actttcgaag
     7741 tctgggacca agaatacatg ctttaccaga aagtgtatca atattcagtt tgaaagagat
     7801 tcagttgcaa aaggacccgg gttttcgaaa ttcttcttat ccagagacag gacatgggaa
     7861 gaacagaaga cagagcatgc tcttttagga cccagctttc ttgtacaaag ttgtgatatc
     7921 cagcacagtg gcggccgctc gacaatcaac ctctggatta caaaatttgt gaaagattga
     7981 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     8041 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     8101 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     8161 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     8221 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     8281 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     8341 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     8401 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     8461 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     8521 ccgcctcccc gcctggaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga
     8581 gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc
     8641 acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg
     8701 ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc
     8761 aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct
     8821 gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct
     8881 ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg
     8941 ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc
     9001 acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc
     9061 ttaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
     9121 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
     9181 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
     9241 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
     9301 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
     9361 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
     9421 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
     9481 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
     9541 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
     9601 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
     9661 cctgacgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat
     9721 ctcgagggcc cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     9781 tttttaaaag aaaagggggg actggaaggg ctagctcact cccaacgaag acaagatctg
     9841 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     9901 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     9961 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    10021 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
    10081 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
    10141 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    10201 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
    10261 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10321 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10381 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
    10441 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
    10501 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
    10561 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
    10621 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
    10681 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
    10741 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
    10801 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
    10861 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
    10921 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
    10981 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcga
//
