LOCUS       sequence-310003-       11200 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of nontargeting shRNA, RFP i670 expression, 
            based on Addgene 12247.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11200)
  TITLE     Human Citron shRNA and controls
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11200)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Lentiviral nontargeting shRNA RFP i670
FEATURES             Location/Qualifiers
     source          1..11200
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1299..1532
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1717..1761
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2146..3324
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2377..3315
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3272..3292
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3372..3489
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3522..3531
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3528..4475
                     /codon_start=1
                     /product="bright monomeric near-infrared fluorescent 
                     protein engineered from a bacterial phytochrome, with an 
                     emission maximum of 670 nm (Shcherbakova et al., 2016)"
                     /label=miRFP670
                     /label=ORF frame 1
                     /translation="MVAGHASGSPAFGTASHSNCEHEEIHLAGSIQPHGALLVVSEHDH
                     RVIQASANAAEFLNLGSVLGVPLAEIDGDLLIKILPHLDPTAEGMPVAVRCRIGNPSTE
                     YCGLMHRPPEGGLIIELERAGPSIDLSGTLAPALERIRTAGSLRALCDDTVLLFQQCTG
                     YDRVMVYRFDEQGHGLVFSECHVPGLESYFGNRYPSSTVPQMARQLYVRQRVRVLVDVT
                     YQPVPLEPRLSPLTGRDLDMSGCFLRSMSPCHLQFLKDMGVRATLAVSLVVGGKLWGLV
                     VCHHYLPRFIRFELRAICKRLAERIATRITALES"
     misc_feature    4495..5083
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4548..4568)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4966..4977)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    5251..5269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5293..5311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5335..5353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5377..5395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    5419..5437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5466..5680
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5601..5620
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5762..5795)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             5815..5995
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6020..6038)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6021..6038)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6328..6347)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6561..6580)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6680..6702
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6740..6758)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6826..6930
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6931..6999,7000..7791)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7149..7168)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7962..8550
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8451..8470
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8704..8721
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(8791..8811)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8796..9125
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8976..9111
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10259..10324
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10454..10474
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    10899..11033
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10936..10955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10990..11009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     1201 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     1261 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     1321 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     1381 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     1441 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     1501 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     1561 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     1621 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     1681 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     1741 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     1801 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     1861 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     1921 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     1981 gacagatcca ttcgattagt gaacggatct cgacggtatc gatcacgaga ctagcctcga
     2041 ccatcgcgat gtcgacgata agctttgcaa agatggataa agttttaaac agagaggaat
     2101 ctttgcagct aatggacctt ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt
     2161 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
     2221 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
     2281 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
     2341 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
     2401 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
     2461 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
     2521 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
     2581 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
     2641 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
     2701 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
     2761 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
     2821 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
     2881 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
     2941 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
     3001 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
     3061 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     3121 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     3181 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     3241 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     3301 tttcttccat ttcaggtgtc gtgaggaatt tcgacattta aatttaatta atctcgacgg
     3361 tatcggttaa cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     3421 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     3481 caaaatttta tcgatcacga gactagcctc gaggtttaaa cgccaccatg gtagcaggtc
     3541 atgcctctgg cagccccgca ttcgggaccg cctctcattc gaattgcgaa catgaagaga
     3601 tccacctcgc cggctcgatc cagccgcatg gcgcgcttct ggtcgtcagc gaacatgatc
     3661 atcgcgtcat ccaggccagc gccaacgccg cggaatttct gaatctcgga agcgtactcg
     3721 gcgttccgct cgccgagatc gacggcgatc tgttgatcaa gatcctgccg catctcgatc
     3781 ccaccgccga aggcatgccg gtcgcggtgc gctgccggat cggcaatccc tctacggagt
     3841 actgcggtct gatgcatcgg cctccggaag gcgggctgat catcgaactc gaacgtgccg
     3901 gcccgtcgat cgatctgtca ggcacgctgg cgccggcgct ggagcggatc cgcacggcgg
     3961 gttcactgcg cgcgctgtgc gatgacaccg tgctgctgtt tcagcagtgc accggctacg
     4021 accgggtgat ggtgtatcgt ttcgatgagc aaggccacgg cctggtattc tccgagtgcc
     4081 atgtgcctgg gctcgaatcc tatttcggca accgctatcc gtcgtcgact gtcccgcaga
     4141 tggcgcggca gctgtacgtg cggcagcgcg tccgcgtgct ggtcgacgtc acctatcagc
     4201 cggtgccgct ggagccgcgg ctgtcgccgc tgaccgggcg cgatctcgac atgtcgggct
     4261 gcttcctgcg ctcgatgtcg ccgtgccatc tgcagttcct gaaggacatg ggcgtgcgcg
     4321 ccaccctggc ggtgtcgctg gtggtcggcg gcaagctgtg gggcctggtt gtctgtcacc
     4381 attatctgcc gcgcttcatc cgtttcgagc tgcgggcgat ctgcaaacgg ctcgccgaaa
     4441 ggatcgcgac gcggatcacc gcgcttgaga gctaacgggg actagtcata tgataatcaa
     4501 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     4561 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     4621 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     4681 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     4741 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     4801 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     4861 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     4921 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     4981 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     5041 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcggta cgtatggcca
     5101 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5161 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatggga tcaattcacc
     5221 atgctagtgg atccccctcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     5281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     5341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     5401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg gggctgcagg
     5461 aattcgaacg ctgacgtcat caacccgctc caaggaatcg cgggcccagt gtcactaggc
     5521 gggaacaccc agcgcgcgtg cgccctggca ggaagatggc tgtgagggac aggggagtgg
     5581 cgccctgcaa tatttgcatg tcgctatgtg ttctgggaaa tcaccataaa cgtgaaatgt
     5641 ctttggattt gggaatctta taagttctgt atgagaccac gcgccctctc gcttgggcga
     5701 gagtaagttc aagagactta ctctcgccca agcgagattt ttacgatacc gtcgcatggg
     5761 aataacttcg tatagcatac attatacgaa gttatgctgc tttttgcttg tactgggtct
     5821 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     5881 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     5941 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc
     6001 tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg
     6061 ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat
     6121 ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg
     6181 ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac
     6241 caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca
     6301 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
     6361 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
     6421 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
     6481 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
     6541 gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     6601 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     6661 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     6721 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     6781 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6841 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6901 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6961 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     7021 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     7081 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     7141 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     7201 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     7261 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     7321 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     7381 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     7441 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     7501 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     7561 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     7621 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     7681 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     7741 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     7801 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7861 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7921 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7981 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     8041 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     8101 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     8161 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     8221 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     8281 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     8341 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     8401 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     8461 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     8521 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     8581 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     8641 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     8701 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     8761 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     8821 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     8941 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     9001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     9061 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     9121 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     9181 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     9241 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     9301 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     9361 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     9421 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     9481 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     9541 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     9601 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     9661 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     9721 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     9781 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     9841 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     9901 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     9961 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
    10021 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
    10081 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
    10141 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
    10201 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
    10261 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
    10321 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
    10381 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
    10441 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
    10501 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
    10561 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
    10621 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
    10681 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
    10741 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
    10801 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
    10861 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
    10921 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
    10981 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
    11041 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
    11101 attacctgtg gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa
    11161 gaaattgtat ttgttaaata tgtactacaa acttagtagt
//
