LOCUS       sequence-31004-e         907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 907)
  AUTHORS   Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte 
            EM, Voigt CA, Ellington AD
  TITLE     A synthetic genetic edge detection program.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1272-81.
  PUBMED    19563759
REFERENCE   2  (bases 1 to 907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1272-81."
COMMENT     Sequence Label: pJT105
FEATURES             Location/Qualifiers
     source          1..907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(174..278)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     343..361
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(365..408)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    411..432
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             538..549
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(572..594)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
       61 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
      121 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
      181 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
      241 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttcccat ggtgccacct
      301 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
      361 cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg gaattcgcgg
      421 ccgcttctag agtaagcacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      481 tagtcgaata acaccgtgcg tgttgactat tttacctctg gcggtgatat actggagaaa
      541 gaggagaaat actagagatg atagatcccg tcgttttaca acgtcgtgac tgggaaaacc
      601 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      661 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      721 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
      781 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
      841 tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
      901 cgacggg
//
