LOCUS       sequence-310100-       14929 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing Human ACE2 and TMPRSS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14929)
  TITLE     Best Lab Plasmids
REFERENCE   2  (bases 1 to 14929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPI-IRES-Puro-Ak-ACE2-TMPRSS2
FEATURES             Location/Qualifiers
     source          1..14929
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    674..799
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1292..1525
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1710..1754
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2137..3315
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2368..3306
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3263..3283
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3363..3480
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7509..8076
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    7527..8078
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7671..7688)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7898..7917
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8081..8680
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8081..8100)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8577..8597
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(8722..8738)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8723..8739)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    8752..9340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8805..8825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9223..9234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(9484..9517)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9537..9717
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9742..9760)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9743..9760)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10050..10069)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(10283..10302)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10402..10424
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10462..10480)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10548..10652
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10653..10721,10722..11513)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10871..10890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11684..12272
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12173..12192
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12426..12443
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(12513..12533)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12518..12847
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12698..12833
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13981..14046
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             14176..14196
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    14621..14755
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14658..14677)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     14712..14731
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(14923..14929,1..627)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
       61 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
      121 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
      181 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
      241 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
      301 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
      361 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
      421 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
      481 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      541 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      601 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
      661 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
      721 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
      781 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
      841 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
      901 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
      961 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1021 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1081 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1141 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1201 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1261 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1321 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1381 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1441 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1501 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1561 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     1621 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     1681 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     1741 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     1801 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     1861 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     1921 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     1981 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagcct cgaccatcga
     2041 tgtcgacgat aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc
     2101 taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca
     2161 gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt
     2221 gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt
     2281 tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt
     2341 cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc
     2401 ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg
     2461 attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa
     2521 ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg
     2581 cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa
     2641 aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc
     2701 caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg
     2761 tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg
     2821 gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc
     2881 ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg
     2941 cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg
     3001 ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac
     3061 tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg
     3121 tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg
     3181 gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt
     3241 gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca
     3301 tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg gtatcggtta
     3361 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     3421 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     3481 atcgatcacg agactagcct cgaggtttaa acccgggagc gccaccatgt caagctcttc
     3541 ctggctcctt ctcagccttg ttgctgtaac tgctgctcag tccaccattg aggaacaggc
     3601 caagacattt ttggacaagt ttaaccacga agccgaagac ctgttctatc aaagttcact
     3661 tgcttcttgg aattataaca ccaatattac tgaagagaat gtccaaaaca tgaataatgc
     3721 tggggacaaa tggtctgcct ttttaaagga acagtccaca cttgcccaaa tgtatccact
     3781 acaagaaatt cagaatctca cagtcaagct tcagctgcag gctcttcagc aaaatgggtc
     3841 ttcagtgctc tcagaagaca agagcaaacg gttgaacaca attctaaata caatgagcac
     3901 catctacagt actggaaaag tttgtaaccc agataatcca caagaatgct tattacttga
     3961 accaggtttg aatgaaataa tggcaaacag tttagactac aatgagaggc tctgggcttg
     4021 ggaaagctgg agatctgagg tcggcaagca gctgaggcca ttatatgaag agtatgtggt
     4081 cttgaaaaat gagatggcaa gagcaaatca ttatgaggac tatggggatt attggagagg
     4141 agactatgaa gtaaatgggg tagatggcta tgactacagc cgcggccagt tgattgaaga
     4201 tgtggaacat acctttgaag agattaaacc attatatgaa catcttcatg cctatgtgag
     4261 ggcaaagttg atgaatgcct atccttccta tatcagtcca attggatgcc tccctgctca
     4321 tttgcttggt gatatgtggg gtagattttg gacaaatctg tactctttga cagttccctt
     4381 tggacagaaa ccaaacatag atgttactga tgcaatggtg gaccaggcct gggatgcaca
     4441 gagaatattc aaggaggccg agaagttctt tgtatctgtt ggtcttccta atatgactca
     4501 aggattctgg gaaaattcca tgctaacgga cccaggaaat gttcagaaag cagtctgcca
     4561 tcccacagct tgggacctgg ggaagggcga cttcaggatc cttatgtgca caaaggtgac
     4621 aatggacgac ttcctgacag ctcatcatga gatggggcat atccagtatg atatggcata
     4681 tgctgcacaa ccttttctgc taagaaatgg agctaatgaa ggattccatg aagctgttgg
     4741 ggaaatcatg tcactttctg cagccacacc taagcattta aaatccattg gtcttctgtc
     4801 acccgatttt caagaagaca atgaaacaga aataaacttc ctgctcaaac aagcactcac
     4861 gattgttggg actctgccat ttacttacat gttagagaag tggaggtgga tggtctttaa
     4921 aggggaaatt cccaaagacc agtggatgaa aaagtggtgg gagatgaagc gagagatagt
     4981 tggggtggtg gaacctgtgc cccatgatga aacatactgt gaccccgcat ctctgttcca
     5041 tgtttctaat gattactcat tcattcgata ttacacaagg accctttacc aattccagtt
     5101 tcaagaagca ctttgtcaag cagctaaaca tgaaggccct ctgcacaaat gtgacatctc
     5161 aaactctaca gaagctggac agaaactgtt caatatgctg aggcttggaa aatcagaacc
     5221 ctggacccta gcattggaaa atgttgtagg agcaaagaac atgaatgtaa ggccactgct
     5281 caactacttt gagcccttat ttacctggct gaaagaccag aacaagaatt cttttgtggg
     5341 atggagtacc gactggagtc catatgcaga ccaaagcatc aaagtgagga taagcctaaa
     5401 atcagctctt ggagataaag catatgaatg gaacgacaat gaaatgtacc tgttccgatc
     5461 atctgttgca tatgctatga ggcagtactt tttaaaagta aaaaatcaga tgattctttt
     5521 tggggaggag gatgtgcgag tggctaattt gaaaccaaga atctccttta atttctttgt
     5581 cactgcacct aaaaatgtgt ctgatatcat tcctagaact gaagttgaaa aggccatcag
     5641 gatgtcccgg agccgtatca atgatgcttt ccgtctgaat gacaacagcc tagagtttct
     5701 ggggatacag ccaacacttg gacctcctaa ccagccccct gtttccatat ggctgattgt
     5761 ttttggagtt gtgatgggag tgatagtggt tggcattgtc atcctgatct tcactgggat
     5821 cagagatcgg aagaagaaaa ataaagcaag aagtggagaa aatccttatg cctccatcga
     5881 tattagcaaa ggagaaaata atccaggatt ccaaaacact gatgatgttc agacctcctt
     5941 ttagcgcgcc tgcaggatca gcaggcacca tggctttgaa ctcagggtca ccaccagcta
     6001 ttggacctta ctatgaaaac catggatacc aaccggaaaa cccctatccc gcacagccca
     6061 ctgtggtccc cactgtctac gaggtgcatc cggctcagta ctacccgtcc cccgtgcccc
     6121 agtacgcccc gagggtcctg acgcaggctt ccaaccccgt cgtctgcacg cagcccaaat
     6181 ccccatccgg gacagtgtgc acctcaaaga ctaagaaagc actgtgcatc accttgaccc
     6241 tggggacctt cctcgtggga gctgcgctgg ccgctggcct actctggaag ttcatgggca
     6301 gcaagtgctc caactctggg atagagtgcg actcctcagg tacctgcatc aacccctcta
     6361 actggtgtga tggcgtgtca cactgccccg gcggggagga cgagaatcgg tgtgttcgcc
     6421 tctacggacc aaacttcatc cttcaggtgt actcatctca gaggaagtcc tggcaccctg
     6481 tgtgccaaga cgactggaac gagaactacg ggcgggcggc ctgcagggac atgggctata
     6541 agaataattt ttactctagc caaggaatag tggatgacag cggatccacc agctttatga
     6601 aactgaacac aagtgccggc aatgtcgata tctataaaaa actgtaccac agtgatgcct
     6661 gttcttcaaa agcagtggtt tctttacgct gtatagcctg cggggtcaac ttgaactcaa
     6721 gccgccagag caggatcgtg ggcggcgaga gcgcgctccc gggggcctgg ccctggcagg
     6781 tcagcctgca cgtccagaac gtccacgtgt gcggaggctc catcatcacc cccgagtgga
     6841 tcgtgacagc cgcccactgc gtggaaaaac ctcttaacaa tccatggcat tggacggcat
     6901 ttgcggggat tttgagacaa tctttcatgt tctatggagc cggataccaa gtagaaaaag
     6961 tgatttctca tccaaattat gactccaaga ccaagaacaa tgacattgcg ctgatgaagc
     7021 tgcagaagcc tctgactttc aacgacctag tgaaaccagt gtgtctgccc aacccaggca
     7081 tgatgctgca gccagaacag ctctgctgga tttccgggtg gggggccacc gaggagaaag
     7141 ggaagacctc agaagtgctg aacgctgcca aggtgcttct cattgagaca cagagatgca
     7201 acagcagata tgtctatgac aacctgatca caccagccat gatctgtgcc ggcttcctgc
     7261 aggggaacgt cgattcttgc cagggtgaca gtggagggcc tctggtcact tcgaagaaca
     7321 atatctggtg gctgataggg gatacaagct ggggttctgg ctgtgccaaa gcttacagac
     7381 caggagtgta cgggaatgtg atggtattca cggactggat ttatcgacaa atgagggcag
     7441 acggctaggg atccatatgc ggaccgtacg tactagtggc cacggctgat catacgggct
     7501 gcaggaattc cgcccccccc cccctaacgt tactggccga agccgcttgg aataaggccg
     7561 gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc
     7621 ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa
     7681 aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag
     7741 acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg
     7801 cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg
     7861 ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa
     7921 caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg
     7981 gtgcacatgc tttacatgtg tttagtcgag gttaaaaaac gtctaggccc cccgaaccac
     8041 ggggacgtgg ttttcctttg aaaaacacga tgataatacc atgaccgagt acaagcccac
     8101 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8161 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     8221 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     8281 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     8341 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     8401 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     8461 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     8521 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     8581 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     8641 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgggcgcgtc tggaacttcg
     8701 aattcgatat caagcttatc gcgataccgt cgacctcgag ggaattccga taatcaacct
     8761 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8821 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8881 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8941 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     9001 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     9061 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     9121 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     9181 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     9241 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     9301 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgggaatt cgagctcggt
     9361 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     9421 ggggggactg gaagggctaa ttcactccca acgaagacaa gatgggatca attcaccatg
     9481 ggaataactt cgtatagcat acattatacg aagttatgct gctttttgct tgtactgggt
     9541 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     9601 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     9661 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     9721 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     9781 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     9841 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     9901 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     9961 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
    10021 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
    10081 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
    10141 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
    10201 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
    10261 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
    10321 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    10381 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    10441 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
    10501 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
    10561 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
    10621 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
    10681 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
    10741 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
    10801 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
    10861 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
    10921 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
    10981 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
    11041 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
    11101 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
    11161 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
    11221 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
    11281 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
    11341 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
    11401 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
    11461 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
    11521 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
    11581 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
    11641 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
    11701 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
    11761 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
    11821 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
    11881 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
    11941 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
    12001 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
    12061 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
    12121 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
    12181 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
    12241 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
    12301 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
    12361 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
    12421 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
    12481 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
    12541 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    12601 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    12661 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    12721 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    12781 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    12841 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
    12901 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
    12961 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
    13021 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
    13081 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
    13141 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
    13201 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
    13261 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
    13321 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
    13381 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
    13441 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
    13501 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
    13561 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
    13621 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
    13681 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
    13741 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
    13801 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
    13861 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
    13921 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
    13981 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    14041 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    14101 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    14161 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    14221 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    14281 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    14341 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    14401 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    14461 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    14521 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    14581 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    14641 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    14701 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    14761 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    14821 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
    14881 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaagg
//
