LOCUS       sequence-31015-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich 
            A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, 
            Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, 
            Cookson MR
  TITLE     Kinase activity is required for the toxic effects of mutant 
            LRRK2/dardarin.
  JOURNAL   Neurobiol Dis. 2006 Aug . 23(2):329-41.
  PUBMED    16750377
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis. 2006 Aug . 23(2):329-41."
COMMENT     Sequence Label: pDEST53-LRRK2-G2019S
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(95..97,98..100,101..811)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(128..156)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     751..771
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    833..857
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctaga caccatggcc agcaaaggag aagaactttt
      121 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
      181 tgtcagtgga gagggtgaag gtgatgctac atacggaaag cttaccctta aatttatttg
      241 cactactgga aaactacctg ttccatggcc aacacttgtc actactttct cttatggtgt
      301 tcaatgcttt tcccgttatc cggatcatat gaaacggcat gactttttca agagtgccat
      361 gcccgaaggt tatgtacagg aacgcactat atctttcaaa gatgacggga actacaagac
      421 gcgtgctgaa gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaaaggtat
      481 tgattttaaa gaagatggaa acattctcgg acacaaactc gagtacaact ataactcaca
      541 caatgtatac atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg
      601 ccacaacatt gaagatggat ccgttcaact agcagaccat tatcaacaaa atactccaat
      661 tggcgatggc cctgtccttt taccagacaa ccattacctg tcgacacaat ctgccctttc
      721 gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg
      781 gattacacat ggcatggatg agctctacaa aagcggttcc ggaccggatc aaacaagttt
      841 gtacaaaaaa gcaggctccg aattcgccct tatggctagt ggcagctgtc aggggtgcga
      901 agaggacaga
//
